|
| 5th Floor, Building #10. Tel/Fax: 03-5802-1111, Extension: 3622 |
|
| Welcome to the Division of Biomedical Imaging Research (DBIR) |
|---|
| DBIR is one of the specialized laboratories of Biomedical Research Center of Juntendo University. It provides sophisticated bio-imaging techniques that are of increasing importance in biomedical research. DBIR supports studies for many morphological researchers such as graduate students, basic and clinical researchers of our university. DBIR supplies the space, equipment and necessary materials for experiments. Our staff carry out, upon request, routine procedures such as dye- and immunostaining of paraffin-embedded tissue sections. In addition to these services, we give to the users advice and information on current techniques and topics in the field of pathology. |
|
| SPECIMEN PREPARATION SERVICES |
|---|
| We support the preparation of various specimens, except frozen sections that must be made by the user himself. We request the user to bring the samples and fill out the application form. Please make sure that we strictly do not accept the pathogen-infected samples. |
|
|
Histopathological specimen preparation
We provide high quality specimens stained with HE, AZAN, or other specific staining methods. We also accept the preparation of immunohistochemical specimes upon the request.
(The shown image is PAS of human glomerule) |
|
Neuropathological specimen preparations
In addition, we provide specimens of large section such as central nervous tissue.
(Shown is large section of KB stain of human cerebellum + pons) |
|
Immunohistochemical specimen preparation
In our laboratory, we mainly perform the enzyme immunohistochemical specimes using paraffin sections.
(The figure shows an immunohistochemical staining with anti-neurofilament antibody in a ventral horn cell of the ALS patient�j |
|
Histochemical specimen preparation.
We perform several kinds of histochemical specimens such as TUNEL, DOPA reaction and AChE.
(Figure: TUNEL method of mouse cornea infected with HSV) |
|
In situ hybridization (ISH) & FISH
We make ISH & FISH, however, it is requested to the user to bring the prepared probe.�iShown is EB virus of gastric cancer detected by in situ hybridization using EBER1 probe�j |
| RESEARCH SUPPORT |
|---|
|
|
DBIR provides an excellent environment to develop the bio-imaging experiments. Our staff have expertise and technology to wholeheartedly support the users for various experiments such as histopathology or immunohistochemistry.
We support the research in the following fields:
1. General pathology
2. Neuropathology
3. Immunohistochemistry
4. Histochemistry
5. Molecular pathology
6. Computerized image analysis
|
|
|
| Main Instruments |
|---|
|
Optical microscope and camera system (Nikon, Olympus)
Fluorescence microscope and high sensitive CCD camera system (Zeiss, Nikon, Olympus)
Cryostat (Leica)
Vibratome (D.S.K.EM)
Cytospin (Bright)
Microwave processor (Azumaya Medicals)
Autoimmunostaining system (Ventana Medical Systems Japan)
Image analyzer KS400 (Zeiss)
PCR/in situ PCR system (MJ Research)
Paraffin Processing and Embedding system (Sakura Finetek)
|
|
