Research fields in this laboratory include serology and DNA polymorphisms, pathophysiologic assessment of lipid peroxidation by immunoassay. Our current topics are contributing to the development and the improvement of forensic science investigations.
1) Development of immunoassay and its application to forensic science investigations
We are developing new highly sensitive immunoassay methods, i. e. quantitative latex immunoassay, laser induced photo-thermal immunoassay, time resolved fluoroimmunoassay with a new labeling material and applying these methods to forensic science investigations.
2) Development of DNA analysis and its application to forensic science investigations
We are studying effective PCR-based methods, such as sequence specific PCR with positive control (SSPPC) and confronting two pair primers (CTPP) for ABO, Lewis, secretor & nonseretor blood groupings using the fragment analysis. We are also studying primer extension preamplification to prepare copied DNA specimens, whether they are amplified correctly for the subsequent PCR analyses. Many DNA analyses and sample preservation will be performed from a small DNA specimen.
3) Development of novel DNA markers that are acquired in people after birth (acquired DNA marker)
To establish novel DNA markers, we chose the Epstein-Barr(EB) virus. Because the EB virus is acquired and easily identified from a host, personal identification can be performed by acquired DNA markers.
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