Juntendo University, Tokyo, established in 1838.

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Department of Cell Biology and Neuroscience

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Faculties

Professor and Chairman:
Masato KOIKE, M.D., Ph,D.
e-mail: mkoike@juntendo.ac.jp
Tel: +81-3-5802-1024
Fax: +81-3-5800-0245
Research Professor:
Yasuo UCHIYAMA, M.D., Ph,D.
Associate Professor:
Isei TANIDA, Ph,D.
Hiroyuki HIOKI , M.D., Ph,D.
Assistant Professor:
Takehiko Sunabori, Ph,D.
Yu-Shin Sodaka, Ph,D.
Mutsumi Yokota, Ph,D.
Laxmi Kumar Parajuli, Ph,D.
Lecturer (Non-Full-time):
Yoshiro TAKANO, D.D.S., Ph,D.

Research

Our research aims to understand how genetic mutations lead to cellular disorganization and disease, with special emphasis on neurodegenerative diseases related to autophagy, endo-lysosomes, and secretory pathways. Our research team integrates cell and molecular biology, biochemistry genetics, and light and electron microscopy.

1) Masato Koike (Professor)

We have been analyzing brain tissues from cathepsin D deficient mice, a model for the most severe type of neuronal ceroid lipofuscinoses (NCLs). NCLs are classified as rare inherited neurodegenerative diseases characterized with accumulation of mitochondrial proteins in lysosomes. However, taking into account that in many cases of early-onset familial Parkinson's disease (PD), genes essential for mitophagy are mutated, NCL and PD may share etiological features. Indeed, mutations in ATP13A2 gene are a known cause of Kufor-Rakeb syndrome (KRS) with both PD phenotypes and NCL pathology. We are going to continue genetic study of mouse models with impairment of autophagy and/or lysosomal proteolysis for better understanding of common neurodegenerative diseases.

2) Masato Koike (Professor)

Currently, the demand for the techniques bridging the gap between light and electron microscopy is expanding in the biomedical field. In collaboration with individuals at the Laboratory of Morphology and Image Analysis, one of the biggest and best equipped support facilities in Japanese medical schools, we are establishing more practical CLEM (correlative light and electron microscopy) method and applying 3D imaging of cells and tissues by focused ion beam/scanning electron microscopy (FIB/SEM). Furthermore, in collaboration with Prof. Klumperman, a chair of the department of cell biology and cell microscopy core, medical center Utrecht, where Prof. Koike was staying in 2014 as a visiting professor, we promote advanced Tokuyasu cryosectioning techniques for CLEM.

3) Isei Tanida (Associate Professor)

We focused on the molecular mechanism in the formation of autophagosomes during autophagy, especially Atg7-mediated ubiqutylation-like reactions. In addition, we are developing tools for monitoring autophagy.

4) Takehiko Sunabori (Assistant Professor)

We started to analyze brain-specific conditional knockout (cKO) mouse deficient in p18/LAMTOR1, a late endosome/lysosome-specific anchor protein for the mTORC1/MAPK signaling pathway.

5) Yu-Shin Sodaka (Assistant Professor)

We started to analyze brain- and pancreatic beta cell-specific cKO mouse deficient in Golgi pH regulator (GPHR), an anion channel critical for acidification and functions of the Golgi apparatus.

Selected Publications

  • Koike, M., Shibata, M., Sunabori, T., Yamaguchi, J., Sakimura, K., Komatsu, M., Tanaka, K., Uchiyama, Y. Purkinje cells are more vulnerable to the specific depletion of cathepsin D than to that of Atg7. Am. J. Pathol. in press.
  • Koike, M., Kawahara, A., Shibata, M., Uchiyama, Y. Induction of autophagy in the hippocampus after hypoxic-ischemic injury to neonatal rats. Arch. Histol. Cytol. 77(1):13-23, 2017.
  • Tsuboyama, K.*, Koyama-Honda, I.*, Sakamaki, Y., Koike, M., Morishita, H., Mizushima, N. The ATG conjugation systems are important for degradation of the inner autophagosomal membrane. Science 354(6315):1036-41, 2016. (*These authors contributed equally to this work)
  • Sato, S., Koike, M., Funayama, M., Ezaki, J., Fukuda, T., Ueno, T., Uchiyama, Y., Hattori, N. Lysosomal storage of subunit c of mitochondrial ATP synthase in brain-specific ATP13a2-deficient mice. Am. J. Pathol. 186(12):3074-82, 2016.
  • Sunabori, T., Koike, M., Asari, A., Oonuki, Y., Uchiyama, Y. Suppression of ischemia-induced hippocampal pyramidal neuron death by hyaluronan tetrasaccharide through inhibition of Toll-like receptor 2 signaling pathway. Am. J. Pathol. 186(8):2143-51, 2016.
  • Shibata, M., Koike, M., Kusumi, S., Sato, N., Uchiyama, Y.A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney. Arch Histol Cytol. 76(1):1-8, 2016.
  • Yamamoto-Nonaka K., Koike, M., Asanuma, K., Akiba-Takagi, M., Trejo, JA., Seki, T., Hidaka, T., Ichimura, K., Sakai, T., Tada, N., Ueno, T., Uchiyama, Y., Tomino, Y.
  • Xie, C., Ginet, V., Sun, Y., Koike, M., Zhou, K., Li, T., Li, H., Li, Q., Wang, X., Uchiyama, Y., Truttmann, AC., Kroemer, G., Puyal, J., Blomgren, K., Zhu, C. Neuroprotection by selective neuronal deletion of autophagy-related gene ATG7 in neonatal brain injury. Autophagy 12(2):410-23, 2016.
  • Rinchai, D., Riyapa, D., Buddhisa, S., Utispan, K., Titball, R.W., Stevens, P.M., Stevens, J.M., Ogawa, M., Tanida, I., Koike, M., Uchiyama, Y., Ato, M., Lertmemongkolchai, G. Macroautophagy is essential for killing of Burkholderia pseudomallei in human neutrophils. Autophagy 11(5):748-55, 2015.
  • Nanao, T.*, Koike, M.*, Yamaguchi, J., Sasaki, M., Uchiyama, Y. Cellular localization and tissue distribution of endogenous DFCP1 protein. Biomed. Res. 36(2):121-33, 2015. (*These authors contributed equally to this work)
  • Uemura, N., Koike, M., Asai, K., Kinoshita, M., Fujiwara-Ishikawa, T., Matsui, H., Naruse, K., Sakamoto, N., Uchiyama, Y., Todo, T., Takeda S.I., Yamakado, H., Takahashi, R. Viable neuronopathic Gaucher disease model in medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein. Plos Genet. 11(4):e1005065, 2015.
  • Ichimura, K., Miyazaki, N., Sadayama, S., Murata, K., Koike, M., Nakamura, K., Ohta, K., Sakai, T. Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy. Sci. Rep. 5:8993.
  • Nori, S., Okada, Y., Nishimura, S., Sasaki, T., Itakura, G., Kobayashi, Y., Renault-Mihara, F., Shimizu A., Koya, I., Yoshida, R., Kudoh, J., Koike, M., Uchiyama, Y., Ikeda, E., Toyama, Y., Nakamura, M., Okano, H. Long-term safety issues of iPSC-based cell therapy in a spinal cord injury model: oncogenic transformation with epithelial-mesenchymal transition. Stem. Cell Rep. 4(3):360-73.
  • Asano, T.*, Koike, M.*, Sakata, S., Takeda, Y., Nakagawa, T., Hatano, T., Ohashi, S., Funayama, M., Yoshimi, K., Asanuma, M., Toyokuni, S., Mochizuki, S., Uchiyama, Y., Hattori, N., Iwai, K. Iron induces mitochondrial damage that recruits parkin. Neurosci. Lett. 588:29-35, 2015. (*These authors contributed equally to this work)
  • Suyama, M.*, Koike, M.*, Asaoka, D., Mori, H., Oguro, M., Ueno, T., Nagahara, A., Watanabe, S. Uchiyama, Y. Increased immunoreactivity of cathepsins in the rat esophagus under chronic acid reflux esophagitis. J. Histochem. Cytochem. 62(9):645-60, 2014. (*These authors contributed equally to this work)
  • Shigihara, N.*, Fukunaka, A.*, Hara, A.*, Komiya, K., Honda, A., Uchida, T., Abe, H., Toyofuku, Y., Tamaki, M., Ogihara, T., Miyatsuka, T., Hiddinga, HJ, Sakagashira. S., Koike, M., Uchiyama, Y., Yoshimori, T., Eberhardt, NL, Fujitani, Y.*, Watada, H. Human IAPP-induced pancreatic beta-cell toxicity and its regulation by autophagy. J. Clin. Invest. 124(8):3634-44, 2014. (*These authors contributed equally to this work)
  • Yamanaka, T., Tosaki, A., Kurosawa, M., Matsumoto, G., Koike, M., Uchiyama, Y., Maity, S.N., Hattori, N., Nukina, N. Neuronal NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin/p62 accumulation and endoplasmic reticulum disorganization. Nat. Commun. 5:3354, 2014.
  • Nonomura, K., Yamaguchi, Y., Hamachi, M., Koike, M., Uchiyama, Y., Nakazato, K., Mochizuki, A., Sakaue-Sawano, A., Miyawaki, A., Yoshida, H., Kuida, K., Miura, M. Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells. Dev. Cell 27(6):621-34, 2013.
  • Koike, M.*, Tanida, I.*, Nanao, T., Tada, N., Iwata, J., Ueno, T., Kominami, E., Uchiyama, Y. Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons. PLoS One 8(5):e63568, 2013. (*These authors contributed equally to this work)
  • Ohkouchi, S., Shibata, M., Sasaki, M., Koike, M., Saftig, P., Peters, C., Nagata, S., Uchiyama, Y. Biogenesis and proteolytic processing of lysosomal DNase II. PLoS One 8(3):e59148, 2013.
  • Koike, M., Shibata, M., Ezaki, J., Peters, C., Saftig, P., Kominami, E., Uchiyama, Y. Differences in expression patterns of cathepsin C/dipeptidyl peptidase I in normal, pathological and aged mouse central nervous systems. Eur. J. Neurosci. 37(5):816-20,2013.
  • Piao, X., Komazawa-Sakon, S., Nishida, T., Koike, M., Piao JH., Ehlken, H., Kurihara, H., Hara, M., van Rooijen, N., Schütz, G., Ohmuraya, M., Uchiyama, Y., Yagita, H., Okumura, K., He YW., Nakano, H. c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Sci. Signal 5 (255); ra93, 2012.
  • Tashiro Y.*, Urushitani, M.*, Inoue, H., Koike, M., Uchiyama, Y., Komatsu, M., Tanaka, K., Yamazaki, M., Abe, M., Misawa, H., Sakimura, K., Ito, H., Takahashi, R. Motor neuron-specific disruption of proteasomes, but not autophagy, replicates amyotrophic lateral sclerosis. J. Biol. Chem. in press. doi:10.1074/jbc.M112.417600 (*These authors contributed equally to this work)
  • Imaizumi, Y., Okada, Y., Akamatsu, W., Koike, M., Kuzumaki, N., Hayakawa, H., Nihira, T., Kobayashi, T., Ohyama, M., Sato, S., Takanashi, M., Funayama, M., Hirayama, A., Soga, T., Hishiki, T., Suematsu, M., Yagi, T., Ito, D., Kosakai, A., Hayashi, K., Shouji, M., Nakanishi, A., Suzuki, N., Mizuno, Y., Mizushima, N., Amagai, M., Uchiyama, Y., Mochizuki, H., Hattori, N., Okano, H. Mitochondrial dysfunction with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain. Molecular Brain 5(1):35, 2012.
  • Unno, T., Wakamori, M., Koike, M., Uchiyama, Y., Ishikawa, K., Kubota, H., Yoshida, T., Sasakawa, H., Peters, C., Mizusawa, H., Watase, K. The development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6. Proc. Natl. Acad. Sci. USA 109(43):17693-8, 2012.
  • Sekine, S., Kanamaru, Y., Koike, M., Nishihara, A., Okada, M., Kinoshita, H., Kamiyama, M., Maruyama, J., Uchiyama, Y., Ishihara, N., Takeda, K., Ichijo, H. Rhomboid protease PARL mediates the mitochondrial membrane potential loss-induced cleavage of PGAM5. J. Biol. Chem. 287(41):34635-45, 2012.
  • Mori, H.*, Koike, M.*, Gotow, T., Ichimura, K., Asaoka, D., Oguro, M., Nagahara, A., Ueno, T., Uchiyama, Y., Watanabe, S. Ultrastructural analyses of the rat esophageal stratified epithelium under normal conditions and chronic reflux esophagitis. Arch. Histol. Cytol. in press. (*These authors contributed equally to this work)
  • Nori, S., Okada, Y., Yasuda, A., Tsuji, O., Takahashi, Y., Kobayashi, Y., Fujiyoshi, K., Koike, M., Uchiyama, Y., Ikeda, E., Toyama, Y., Yamanaka, S., Nakamura, M., Okano, H. Grafted human induced pluripotent stem-cell-derived neurospheres promotes motor functional recovery after spinal cord injury in mice. Proc. Natl. Acad. Sci. U S A 108(40): 16825-30, 2011.
  • Uchida, Y., Hasegawa, J., Chinnapen, D., Inoue, T., Okazaki, S., Kato, R., Wakatsuki, S., Misaki, R., Koike, M., Uchiyama, Y., Iemura, S., Natsume, T., Kuwahara, R., Nakagawa, T., Nishikawa, K., Mukai, K., Miyoshi, E., Taniguchi, N., Sheff, D., Lencer, W.I., Taguchi, T., Arai H. Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes. Proc. Natl. Acad. Sci. U S A 108(38): 15846-51, 2011.
  • Kaneko, N., Marin, O., Koike, M., Hirota, Y., Uchiyama, Y., Wu, J.Y., Lu, Q., Tessier-Levigne, M., Alvarez-Buylla, A., Okano, H., Rubenstein, J.L., Sawamoto, K. New neurons clear the path of astrocytic processes for their rapid migration in the adult brain. Neuron 67(2): 213-23; 2010.
  • Sasaki, K., Hamasaki, J., Koike, M., Hirano, Y., Komatsu, M., Uchiyama, Y., Tanaka, K., Murata, S. PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis. Mol. Cell Biol. 30(15): 3864-74; 2010.
  • Shibata, M., Yoshimura, K., Tamura, H., Ueno, T., Nishimura, T., Inoue, T., Sasaki, M., Koike M., Arai, H., Kominami, E., Uchiyama, Y. LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation. Biochem. Biophys. Res. Commun. 393(2): 274-9; 2010.
  • Tamura, H., Shibata, M., Koike, M., Sasaki, M., Uchiyama, Y. Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains. J. Histochem. Cytochem. 58(5): 443-53; 2010.
  • Maejima, Y., Sedbazar, U., Suyama, S., Kohno, D., Onaka, T., Takano, E., Yoshida, N., Koike, M., Uchiyama, Y., Fujiwara, K., Takahashi, N., Horvath, T.L., Dietrich, M.O., Tanaka, S., Dezaki, K., Oh-I, S., Hashimoto, K., Shimizu, H., Nakata, M., Mori, M., Yada, T. Nesfatin-1-regulated oxytocinergic signaling in the paraventricular nucleus causes anorexia through a leptin-independent melanocortin pathway. Cell Metab. 10(5): 355-65; 2009.
  • Shibata, M.*, Yoshimura, K.*, Furuya, N., Koike, M., Ueno, T., Komatsu, M., Arai, H., Tanaka, K., Kominami, E., Uchiyama, Y. The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biochem. Biophys. Res. Commun. 382(2): 419-23; 2009. (*These authors contributed equally to this work)
  • Nada, S.*, Hondo, A. *, Kasai, A.*, Koike, M.*, Saito, K., Uchiyama, Y., Okada, M. The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes. EMBO J. 28(5): 477-89; 2009. (*These authors contributed equally to this work)
  • Gotoh, K., Lu, Z., Morita, M., Shibata, M., Koike, M., Waguri, S., Dono, K., Kominami, E., Sugioka, A., Monden, M., Uchiyama, Y. Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation. Autophagy 5(3): 351-60; 2009.
  • Maeda, Y., Ide T., Koike, M., Uchiyama, Y., Kinoshita, T. GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. Nat. Cell Biol. 10(10): 1135-45; 2008.
  • Mori, Y.*, Koike, M.*, Moriishi, E., Kawabata, A., Tang, H., Oyaizu, H., Uchiyama, Y., Yamanishi, K. Human herpesvirus-6 induces MVB formation, and virus egress occurs by an exosomal release pathway. Traffic 9(10): 1728-42; 2008. (*These authors contributed equally to this work)
  • Oppenheim, R.W., Blomgren, K., Ethell, D.W., Koike, M., Komatsu, M., Prevette, D., Roth, K.A., Uchiyama, Y., Vinsant, S., Zhu, C. Developing postmitotic mammalian neurons in vivo lacking Apaf-1 undergo programmed cell death by a caspase-independent, nonapoptotic pathway involving autophagy. J. Neurosci. 28(6): 1490-7; 2008.
  • Koike, M., Shibata, M., Tadakoshi, M., Gotoh, K., Komatsu, M., Waguri, S., Kawahara, N., Kuida, K., Nagata, S., Kominami, E., Tanaka, K., Uchiyama, Y. Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am. J. Pathol. 172(2): 454-69; 2008.
  • Komatsu, M., Waguri, S., Koike, M., Sou, Y.S., Ueno, T., Hara, T., Mizushima, N., Iwata, J., Ezaki, J., Murata, S., Hamasaki, J., Nishito, Y., Iemura, S., Natsume, T., Yanagawa, T., Uwayama, J., Warabi, E., Yoshida, H., Ishii, T., Kobayashi, A., Yamamoto, M., Yue, Z., Uchiyama, Y., Kominami, E., Tanaka, K. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131(6): 1149-63; 2007.
  • Miyanishi, M., Tada, K., Koike, M., Uchiyama, Y., Kitamura, T., Nagata, S. Identification of Tim-4 as a phosphatidylserine receptor. Nature 450(7168): 435-9; 2007.
  • Nakahara, M., Nagasaka, A., Koike, M., Uchida, K., Kawane, K., Uchiyama, Y., Nagata, S. Degradation of nuclear DNA by DNase II-like acid DNase in cortical fiber cells of mouse eye lens. FEBS J. 274(12): 3055-64; 2007.
  • Yae, K., Keng V.W., Koike, M., Yusa, K., Kouno, M., Uno, Y., Kondoh, G., Gotow, T., Uchiyama, Y., Horie, K., Takeda, J. Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth. Mol. Cell Biol. 26(16): 6185-96; 2006.
  • Yoshimura, K., Shibata, M., Koike, M., Gotoh, K., Fukaya, M., Watanabe, M., Uchiyama, Y. Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues. Autophagy 2(3): 200-8; 2006.
  • Komatsu, M., Waguri, S., Chiba, T., Murata, S., Iwata, J., Tanida, I., Ueno, T., Koike, M., Uchiyama, Y., Kominami, E., Tanaka, K. Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature 441(7095): 880-4; 2006.
  • Koike, M., Shibata, M., Waguri, S., Yoshimura, K., Tanida, I., Kominami, E., Gotow, T., Peters, C., von Figura, K., Mizushima, N., Saftig, P., Uchiyama, Y. Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease). Am. J. Pathol. 167(6): 1713-28; 2005.
  • Yoshida, H., Kawane, K., Koike, M., Mori, Y., Uchiyama, Y., Nagata, S. Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells. Nature 437(7059): 754-8; 2005.
  • Shimizu, T., Hayashi, Y., Yamasaki, R., Yamada, J., Zhang, J., Ukai, K., Koike, M., Mine, K., von Figura, K., Peters, C., Saftig, P., Fukuda, T., Uchiyama, Y., Nakanishi, H. Proteolytic degradation of glutamate decarboxylase mediates disinhibition of hippocampal CA3 pyramidal cells in cathepsin D-deficient mice. J. Neurochem. 94(3): 680-90; 2005.
  • Lu, Z., Dono, K., Gotoh, K., Shibata, M., Koike, M., Marubashi, S., Miyamoto, A., Takeda, Y., Nagano, H., Umeshita, K., Uchiyama, Y., Monden, M. Participation of autophagy in the degeneration process of rat hepatocytes after transplantation following prolonged cold preservation. Arch. Histol. Cytol. 68(1): 71-80; 2005.
  • Hanayama, R., Tanaka, M., Miyasaka, K., Aozasa, K., Koike, M., Uchiyama, Y., Nagata, S. Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice. Science 304(5674): 1147-50, 2004.
  • Kobayashi, K., Takahashi, M., Matsushita, N., Miyazaki, J., Koike, M., Yaginuma, H., Ohsumi, N., Kaibuchi, K., Kobayashi, K. Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase. J. Neurosci. 24(14): 3480-8; 2004.
  • Ezaki, J., Takeda-Ezaki, M., Koike, M., Ohsawa, Y., Taka, H., Mineki, R., Murayama, K., Uchiyama, Y., Ueno, T., Kominami, E. Characterization of Cln3p, the gene product responsible for juvenile neuronal ceroid lipofuscinosis, as a lysosomal integral membrane glycoprotein. J. Neurochem. 87(5): 1296-308; 2003.
  • Koike, M., Shibata, M., Ohsawa, Y., Nakanishi, H., Koga, T., Kametaka, S., Waguri, S., Momoi, T., Kominami, E., Peters, C., von Figura, K., Saftig, P., Uchiyama, Y. Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice. Mol. Cell Neurosci. 22(2): 146-161; 2003.
  • Sakakibara, S., Nakamura, Y., Yoshida, T., Shibata, S., Koike, M., Takano, H., Ueda, S., Uchiyama, Y., Noda, T., Okano, H. RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation. Proc. Natl. Acad. Sci. U S A 99(23): 15194-9; 2002.
  • Koike, M., Shibata, M., Ohsawa, Y., Kametaka, S., Waguri, S., Kominami, E., Uchiyama, Y. The expression of tripeptidyl peptidase I in various tissues of rats and mice. Arch. Histol. Cytol. 65(3): 219-32; 2002.
  • Ogawa, Y., Sawamoto, K., Miyata, T., Miyao, S., Watanabe, M., Nakamura, M., Bregman, B.S., Koike, M., Uchiyama, Y., Toyama, Y., Okano, H. Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats. J. Neurosci. Res. 69(6): 925-33; 2002.
  • Shibata, M., Koike, M., Waguri, S., Zhang, G., Koga, T., Uchiyama, Y. Cathepsin D is specifically inhibited by deoxyribonucleic acids. FEBS letters. 517(1-3): 281-4; 2002.
  • Yoshikawa, M., Uchida, S., Ezaki, J., Rai, T., Hayama, A., Kobayashi, K., Kida, Y., Noda, M., Koike, M., Uchiyama, Y., Marumo, F., Kominami, E., Sasaki, S. CLC-3 deficiency leads to phenotypes similar to human neuronal ceroid lipofuscinosis. Genes Cells 7(6): 597-605; 2002.
  • Nakanishi, H., Zhang, J., Koike, M., Nishioku, T., Okamoto, Y., Kominami, E., von Figura, K., Peters, C., Yamamoto, K., Saftig, P., Uchiyama, Y. Involvement of nitric oxide released from microglia-macrophages in pathological changes of cathepsin D-deficient mice. J. Neurosci. 21(19): 7526-33; 2001.
  • Yayoi, Y., Ohsawa Y., Koike, M., Zhang, G., Kominami E., Uchiyama Y. Specific localization of lysosomal aminopeptidases in type II alveolar epithelial cells of the rat lung. Arch. Histol. Cytol. 64(1): 89-97; 2001.
  • Koike, M.*, Nakanishi, H.*, Saftig, P., Ezaki, J., Isahara, K., Ohsawa, Y., Schulz-Schaeffer, W., Watanabe, T., Waguri, S., Kametaka, S., Shibata, M., Yamamoto, K., Kominami, E., Peters, C., von Figura, K., Uchiyama, Y. Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons. J. Neurosci. 20(18): 6898-906; 2000. (*These authors contributed equally to this work)
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