Laboratory of Morphology and Image Analysis
Facility Guide
Downloading of related documents is available from the following page
Application form for registration, specimen preparation order form, etc.
(Note: Access requires logging into your individual Juntendo Gmail account.)
User Registration
To use this laboratory, it is necessary to complete a registration process by submitting a "User Registration Application Form."
Once registered, you will be able to use your nameplate to enter the room.
How to register
Fill out the user registration application form, affix your department head's seal, and submit it to us on the 5th floor of Building 10.
If you apply by e-mail, please send the electronic file (e.g. PDF) to the person in charge. Before using the facility after registration, you are required to attend a facility usage guidance session (approximately 20 minutes). The person in charge will contact you after you submit the application form.
Please follow the instructions given at the guidance and the “Rules of Use” distributed at the time of the guidance.
(Contact: KAKUTA S., skakuta@juntendo.ac.jp)
User Support
(Note) Bringing in infectious samples or those with potential infection risks is prohibited.
However, samples that have been fixed and whose safety has been confirmed can be brought in if you inform us of this.
(1) Request of Specimen Preparation
We can prepare pathological staining specimen and specimens for electron microscopy.
Please fill out the Order Sheet and submit it with your fixed samples.
＜Light Microscopy＞
1. Pathological staining
In addition to HE staining, special staining is available as much as possible. Requests for preparation of unstained specimens for immunohistochemistry and other purposes are also acceptable.
We accept requests for samples from fixed tissues, already prepared paraffin blocks, and unstained sections.
2. Immunohistochemistry specimens
DAB staining on paraffin sections is the basic method. Primary antibodies must be brought in by the client.
We also accept requests for immunofluorescence staining (IF).
＜Electron microscopy＞
1.Transmission electron microscopy (TEM)
We perform a series of work from embedding of tissue and cultured cells to preparation of ultra-thin sections and electron staining. We also accept requests for embedding (block preparation) only and sectioning from the embedded blocks.
Immunoelectron microscopy and frozen samples are also available upon request, but please consult us about these options.
2. Scanning electron microscopy (SEM)
We provide services from post-fixation and dehydration of specimens to freeze-drying, critical point drying, and metal coating.
A focused ion beam scanning electron microscope (FIB/SEM) has also been introduced to enable 3D analysis by serial block face imaging.
(Notes on preparation of specimens)
Although we will do our best to prepare specimens according to your wishes, we may not be able to meet all of your requests due to the nature of our work.
We cannot be held responsible for any mistakes, especially with valuable specimens, so we recommend that the researcher who knows the characteristics of the specimen prepare the specimen himself/herself.
If the number of requests is extremely large, we may set a limit on the number of requests per course or group.
(2) Research Support
The various research instruments (list of main instruments) and experimental benches installed in our laboratory are available for use. We also provide technical guidance and necessary reagents for users (reagents and consumables are available for a fee with some exceptions). We mainly provide the following support
- Support for preparation of pathology and electron microscopy specimens
- Assistance with immunostaining and HE staining
- Explanation and technical guidance on the handling of microtomes
- Explanation and technical guidance on the handling of microscopes
- Support for image analysis
If you require other special techniques, please consult with our staff.
When using equipment for the first time, you must attend usage instructions/training for each device.
Research equipment and experimental benches operate on a reservation system; please make a reservation before use.
When using USB memory sticks to obtain data from microscopes, please be sure to scan them for viruses using a virus-checking computer before use.
(3) Acknowledgments
If you publish a paper on the results of your research using our facilities, please include an acknowledgement to the laboratory.
We may also ask you to submit a list of your papers for the purpose of preparing a project report. We appreciate your cooperation.
Example of Acknowledgments:
We thank members of the Laboratory of Morphology and Image Analysis, Biomedical Research Core Facilities,Juntendo University Graduate School of Medicine for technical assistance with microscopy.
(4)List of Major Equipment
Light microscope-related equipment
- Cryostat CM3050S (Leica)
- AxioImager 2 (Zeiss) fluorescence microscopes with Apotome
- Optical microscope with digital camera: BX53 with DP74 (Olympus/Evident)
- Image analyzer Definiens / Tissue studio (CTC Laboratory)
- Confocal laser microscope TCSSP5 (Leica)
- Virtual Slide Conference System VS120 (Olympus)
- Stereo fluorescence microscope M165FC (Leica)
- Automated staining systems BenchMark GX (Ventana)
- Multispectral camera system Nuance FX (PerkinElmer)
- Laser microdissection LMD7000 (Leica)
- Sliding microtome ROM380 (Yamato Koki)
- Rotating microtome RM2245 (Leica)
- Tissue processor (paraffin embedding device) ASP6025 (Leica)
Electron Microscope-Related Equipment
- Transmission electron microscope HT7700 (Hitachi)
- Transmission electron microscope JEM-1400Flash (JEOL)
- Scanning electron microscope S-4800 (Hitachi)
- Focused Ion Beam Scanning Electron Microscope (FIB/SEM) Helios660 (Thermo/FEI)
- Ultramicrotome ULTRACUT UC6/7 (Leica)
- Frozen ultramicrotome UC7/FC7 (Leica)
- Vibratome VT1200S (Leica)
- High-pressure freezing apparatus EM HPM100 (Leica)
- Freeze substitution device AFS2 (Leica)
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