Juntendo University, Tokyo, established in 1838.

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Department of Otorhinolaryngology

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Department of Otorhinolaryngology in Juntendo University School of Medicine aims to establish both basic and clinical research especially in the fields of pathophysiology of sensory organs such as the inner ear, immunology of the upper airways, and head and neck oncology.

1.Inner ear pathology

Our goal is to resolve the molecular mechanisms of sensorineural hearing impairment, which is known to show the highest prevalence in the peripheral nervous disorders, and thereby establish the fundamental treatment of inner ear damage. The accumulating evidence for the genetic relevance of sensorineural deafness has been generated by discover of the deafness genes of human studies. We have already reported that the mutations of GJB2 gene and mitochondrial genes are most popular in Japanese deafness peoples. Especially, common mutations of GJB2 gene could be examined by the quantitative real-time PCR for a rapid time and in massive samples, which makes neonatal blood screening of hereditary deafness and provides early treatment of communication disorders. In order to establish the fundamental treatment of genetic deafness, the animal model of GJB2 mutation has been developed. The transgenic approach resulted in the generation of a mouse model of both dominant-negative mutation and recessive mutation. Both mouse models showed a congenital deafness similar to humans. Our future research includes the genetic therapy to rescue the deafness. Another theme of research is a clinical application of a novel type of implantable hearing aid to patients of sensorineural hearing loss, which is developed by the cooperation of The University of Electro-Communications.

Experimental room of hearing test (out side and inside)
Digital microscope
Sound control system
2.Immunology of the upper airways

Our goal is to resolve the molecular mechanisms of sensorineural hearing impairment, which is known to show the highest prevalence in the peripheral nervous disorders, and thereby establish the fundamental treatment of inner ear damage. The accumulating evidence for the genetic relevance of sensorineural deafness has been generated by discover of the deafness genes of human studies. We have already reported that the mutations of GJB2 gene and mitochondrial genes are most popular in Japanese deafness peoples. Especially, common mutations of GJB2 gene could be examined by the quantitative real-time PCR for a rapid time and in massive samples, which makes neonatal blood screening of hereditary deafness and provides early treatment of communication disorders. Upper airway disorders include allergic rhinitis, chronic sinusitis, sleep apnea, etc. We investigated the immunological backgrounds of the upper airway mucosal pathology in the basis of cytokines, inflammatory cells, signal transduction, and membrane physiology. The final goal is to establish the tailor-made medicine and surgery to improve the inflammatory conditions of upper airway mucosa by means of molecular engineering of DNA tip.

3.Head and Neck Oncology

Treatment of head and neck cancer has been tremendously developed by the modern introduction of chemoradiotherapy including super-selective transfusion of massive anti-caner drug to preserve the function. Recent advancement of plastic surgery following a wide resection of head and neck cancer also gave functional and cosmetic benefits. We promote basic research of head and neck oncology to create novel modality of treatment as well as clinical achievement.

Current bibliography

  1. Ikeda K*, Oshima T, Nakabayashi S, Kudo T, Takasaka T, Watanabe M, Sawai T A case of basal cell adenocarcinoma of the parotid gland. Tohoku J Exp Med 186:51-59, 1998.
  2. Ikeda K*, Tanno N, Tamura G, Suzuki H, Oshima T, Shimomura A, Nakabayashi S, Takasaka T. Endoscopic sinus surgery alleviates pulmonary function in patients with asthma associated with chronic sinusitis. Ann Otol Rhinol Laryngol 108:355-359, 1999.
  3. Ikeda K*, Watanabe K, Suzuki H, Oshima T, Tanno N, Shimomura A, Sunose H, Takasaka T, Ikeda H, Yoshimoto T. Nasal airway resistance and olfactory acuity following transshenoidal pituitary surgery. Am J Rhinol 13:45-48, 1999.
  4. Goto S, Oshima T, Ikeda K*, Takasaka T. Expression and localization of the Na+-H+ exchanger in the guinea pig cochlea. Hear Res 128:89-96, 1999.
  5. Hirano, K, Ikeda K*, Kawase T, Oshima T, Kakehata S, Takahashi S, Sato T, Kobayashi T, Takasaka T. Prognosis of sudden deafness with special reference to risk factors of microvascular pathology. Auris Nasus Larynx 26:111-115, 1999.
  6. Ikeda K*, Suzuki H, Oshima T, Takasaka T. Endoscopic endonasal repair of orbital floor fracture. Arch Otolaryngol Head Neck Surg 125:59-63, 1999.
  7. Suzuki H*, Asada Y, Ikeda K, Oshima T, Takasaka T. Inhibitory effect of erythromycin on interleukin-8 secretion from exudated cells in the nasal discharge of patients with chronic sinusitis. Laryngoscope 109:407-710, 1999.
  8. Oshima T*, Ueda N, Ikeda K, Abe K, Takasaka T. Hearing loss with a mitochondrial gene mutation is highly prevalent in Japan. Laryngoscope 109:334-338, 1999.
  9. Monma M, Yamaya M, Sekizawa K, Ikeda K, Suzuki N, Takasaka T, Sasaki H*. Increased carbon monoxide in exhaled air of patients with seasonal allergic rhinitis. Clin Exp Allergy 29:1537-1541, 1999.
  10. Suzuki H*, Goto S, Ikeda K, Oshima T, Furukawa M, Takasaka T. Expression of IL-12 receptor β2 and CD30 in the paranasal sinus mucosa of patients with chronic sinusitis. Eur Resp J 13:1008-1013, 1999.
  11. Ikeda K*, Tabata K, Oshima T, Nishikawa H, Hidaka H, Takasaka T. Unilateral examination of olfactory threshold using the Jet Stream Olfactometer. Auris Nasus Larynx 26:435-439, 1999.
  12. Minowa O#, Ikeda K#, Sugitani Y, Oshima T, Nakai S, Katori Y, Suzuki M, Furukawa M, Kawase T, Zheng Y, Ogura M, Asada Y, Watanabe K, Yamanaka H, Gotoh S, Nishi-Takeshima M, Hamada H, Sugimoto T, Kikuchi T, Takasaka T, Noda T*. Altered cochlear fibrocytes in a mouse model of DFN3 nonsyndromic deafness. Science 285, 1408-1411, 1999 (#: equal contributors).
  13. Furukawa M*, Suzuki H, Ikeda K, Oshima T, Yamaya M, Sasaki H, Takasaka T. Kinin and histamine stimulate Cl secretion in gerbil middle ear epithelium: Connection to otitis media. Hear Res 132:109-116, 1999.
  14. Ogura M*, Kawase T, Ikeda K, Oshima T, Takasaka T, Furuta S, Takahashi S. Profound hearig loss dut to cochlear nerve pathology: diagnosis with combination of OAE and MRI. Laryngoscope, 109:1820-1824, 1999.
  15. Oshima T*, Ikeda K, Takasaka T. Sensorineural hearing loss associated with Byler disease. Tohoku J Exp Med 187:83-88, 1999.
  16. Kudo T*, Ikeda K, Kure S, Matubara Y, Oshima T, Watanabe K, Kawase T, Narisawa K, Takasaka T. New common mutations in the connexin 26 gene (GJB2) in childhood deafness in the Japanese population. Am J Med Genet 90:141-145, 2000.
  17. Suzuki H*, Ikeda K, Homma R, Goto S, Oshima T, Furukawa M, Takasaka T. Prognostic factors of chronic rhinosinusitis under long-term low-dose macrolide therapy. ORL J Otol-Rhino-Laryngol 62:121-127, 2000.
  18. Ikeda K*, Takahashi C, Oshima T, Suzuki H, Satake M, Hidaka H, Takasaka T. Endonasal endoscopic marsupialization of paranasal sinus mucoceles. Am J Rhinol 14:107-111, 2000
  19. Ara SA, Ikeda K*, Oshima T, Suzuki M, Kawase T, Kikuchi T, Takasaka K. Cisplatin-induced apoptosis cell death in Mongolian gerbil cochlea. Hear Res 141: 28-38, 2000.
  20. Oshima T*, Nakajima T, Wada H, Ikeda K, Takasaka T. Characterization of novel and identified genes in guinea pig organ of Corti. Biochem Biophys Res Com 273:84-89, 2000.
  21. Xia An-Ping*, Ikeda K, Katori Y, Oshima T, Kikuchi T, Takasaka T. Expression of connexin 31 in the developing mouse cochlea. NeuroReport 11:2449-2453, 2000.
  22. Sato M, Suzuki M, Oshima T, Ogura M, Shimomura A, Suzuki H, Takasaka T, Ikeda K*. Long-term follow-up of obstructive sleep apnea syndrome following surgery in children and adults. Tohoku J Exp Med, 192:165-172, 2000.
  23. Kakehata S*, Dallos P, Brownell WE, Iwasa KH, Kachar B, Kalinaec F, Ikeda K, Takasaka T. Current concept of outer hair cell motility. Auris Nasus Larynx 27:349-355, 2000.
  24. Watanabe K-I, Takeda K, Katori Y, Ikeda K, Oshima T, Yasumoto K-I, Saito H, Takasaka T, Shibahara S*. Expression of the Sox10 gene during mouse inner ear development. Mol Brain Res 84:141-145, 2000.
  25. Hidaka H*, Ikeda K, Oshima T, Ohtani H, Suzuki H, Tateda M, Takahashi C, Takasaka T. A case of extramedullary plasmacytoma arising from the nasal septum. J Otol Laryngol 114:53-55, 2000.
  26. Ogawa T, Ikeda K*, Watanabe M, Satake M, Oshima T, Suzuki N, Nakano H, Matsuura K, Sato M, Takasaka T. A case report of sinonasal teratocarcinosarcoma. Tohoku J Exp Med 190:51-59, 2000.
  27. Ikeda K*, Ogura M, Oshima T, Suzuki H, Higano S, Takahashi S, Kurosawa H, Hida W, Matsuoka H, Takasaka T. Quantitative assessment of the pharyngeal airway by dynamic magnetic resonance imaging in obstructive sleep apnea syndrome. Ann Otol Rhinol Laryngol, 110:183-189, 2001.
  28. Ara AS, Oshima T, Suzuki M, Kawase T, Takasaka T, Ikeda K*. The expression of apoptosis-related proteins in the aged cochlea of mongolian gerbil. Laryngoscope. 111:528-534, 2001.
  29. Oshima T*, Ikeda K, Furukawa M, Suzuki H, Takasaka T. Expression of the voltage-dependent chloride channel ClC-3 in human nasal tissue. ORL J Otol-Rhino-Laryngol 63:82-86, 2001.
  30. Xia A-P, Katori Y, Oshima T, Watanabe K, Kikuchi T, Ikeda K.* Expression of connexin 30 in the developing mouse cochlea. Brain Res 898:364-367, 2001.
  31. Kudo T*, Ikeda K, Oshima T, Kure S, Tammasaeng M, Prasansuk S, Matsubara Y. 　GJB2 (connexin 26) mutations and children deafness in Thailand. Otol Neurol. 22:858-861, 2001.
  32. Wada H*, Usukura H, Takeuchi S, Sugawara M, Kakehata S, Ikeda K. Distribution of protein motors along the lateral wall of the outer hair cell. Hear Res 162:10-18, 2001.
  33. Oshima T*, Kudo T, Ikeda K. Point mutation of the mitochondrial genome in Japanese deaf-mutism. ORL J Otol-Rhino-Laryngol 63:329-332, 2001.
  34. Baik UB*, Suzuki M, Ikeda K, Sugawara J, Mitani H. Relationship between cephalometric characteristics and obstructive sites in obstructive sleep apnea syndrome. Angle Orthodontist 72:124-134, 2002.
  35. Watanabe K-I, Takeda K, Yasumoto K-I, Udono T, Saito H, Ikeda K, Takasaka T, Takahashi K, Kobayashi T, Tachibana M, Shibahara S*. Identification of a distal enhancer for the melanocyte-specific promoter of the MITF gene. Pigment Cell Res 15:201-211, 2002.
  36. Xia A-P, Kikuchi T, Minowa O, Katori, T, Oshima T, Noda T, Ikeda K*. Late-onset hearing loss in a mouse model of DFN3 non-syndromic deafness: morphological and immmunohistochemical analyses. Hear Res 166:150-158, 2002.
  37. Wada H*, Usukura H, Sugawara M, Katori Y, Kakehata S, Ikeda K, Kobayashi T. Relationship between the local stiffness of the outer hair cell along the cell axis and its ultrastructure observed by atomic force. Hear Res 177:61-70，2003.
  38. Kudo T, Kure S*, Ikeda K, Xia A-P, Katori Y, Kobayashi T, Kojima K, Ichinohe A,, Suzuki Y, Aoki Y, Matsubara Y. Transgenic expression of a dominant-negative connexin26 causes degeneration of the organ of Corti and non-syndromic deafness. Hum Mol Genet 12:995-1004, 2003.
  39. Watanabe K, Oshima T, Kobayashi T, Ikeda K*. The expression and localization of heme oxygenase in the adult guinea pig cochlea. Brain Res 966:162-166, 2003.
  40. Wada H*, Kimura K, Sugawara M, Katori Y, Kakehata S, Ikeda K, Kobayashi T. Imaging of the cortical cytoskeleton of guinea pig outer hair cells using atomic force microscopy, JARO (in press).
  41. Ikeda K, Oshima T, Suzuki H, Kikuchi T, Suzuki M, Kobayashi T. Surgical treatment of subperiosteal abscess of the orbit: Sendai’s ten year experience. Auris Nasus Larynx 30:259-262, 2003.
  42. Kudo T, Oshima T, Kure S, Matsubara Y, Ikeda K. Mutation detection of GJB2 using IsoCode and real-time quantitative polymerase chain reaction with SYBA green I dye for newborn hearing screening. Laryngoscope 114:1299-1304, 2004.
  43. Ikeda K, Gene-based deafness research: Ion transport and hearing. Tohoku J Exp Med, 202:1-11, 2004.
  44. Yoshikawa S, Ikeda K, Kudo T, Kobayashi T The effects of hypoxia, premature birth, infection, ototoxic drugs, circulatory system and congenital disease on neonatal hearing loss. Auris Nasus Larynx. . 31:361-368, 2004.
  45. Nakada K, Sato A, Sone H, Kasahara A, Ikeda K, Kagawa Y, Yonekawa H, Hayashi J-I.Accumulation of pathogenic ΔmtDNA induced deafness but not diabetic phenotypes in mito-mice. BBRC 323:175-184, 2004.
  46. Iida K, Tsumoto K, Ikeda K, Kumagai I, Kobayashi K, Wada H.Construction of an expression system for the motor protein prestin in Chinese hamster ovary cells. Hear Res 205:262-270, 2005.
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