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研究業績詳細

藤本豊士（フジモトトヨシ）

研究テーマ	膜脂質、膜ドメイン、脂肪滴、電子顕微鏡
研究業績（論文）	（原著論文） 1. Osakada H, Fujimoto T. On-grid labeling method for freeze-fracture replicas. Microscopy 72, 56, 2023. 2. Mioka T, Tian G, Wang S, Tsuji T, Kishimoto T, Fujimoto T, Tanaka K. A micron-scale protein-depleted plasma membrane domain could be generated in phosphatidylserine-deficient yeast cells. J Cell Sci 135, jcs256529, 2022. 3. Tsuji T, Fujimoto, T. Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy. STAR Protocol 2, 100990, 2021. 4. Soltysik K, Ohsaki Y, Tatematsu T, Cheng J, Maeda A, Morita S, Fujimoto T. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner. J Cell Biol 220, e202005026, 2021. 5. Orii M, Tsuji T, Ogasawara Y, Fujimoto T. Transmembrane phospholipid translocation mediated by Atg9 is involved in autophagosome formation. J Cell Biol 220, e202009194, 2021. 6. Matoba K, Kotani T, Tsutsumi A, Tsuji T, Mori T, Noshiro D, Sugita Y, Nomura N, Iwata S, Ohsumi Y, Fujimoto T, Nakatogawa H, Kikkawa M, Noda NN. A lipid scramblase mediates autophagosomal membrane expansion. Nat Struct Mol Biol 27, 1185-1193, 2020. 7. Ogasawara Y, Cheng J, Tatematsu T, Uchida M, Murase O, Yoshikawa S, Ohsaki Y, Fujimoto T. Long-term autophagy is sustained by activation of CCTbeta3 on lipid droplets. Nat Commun 11, 4480, 2020. 8. Masuda T, Hirose H, Baba K, Walrant A, Sagan S, Inagaki N, Fujimoto T, Futaki S. An artificial amphiphilic peptide promotes endocytic uptake by inducing membrane curvature. Bioconjug Chem 31, 1611-1615, 2020. 10.1021/acs.bioconjchem.0c00239. 9. Shirakawa R, Goto-Ito S, Goto K, Wakayama S, Kubo H, Sakata N, Trinh DA, Yamagata A, Sato Y, Masumoto H, Cheng J, Fujimoto T, Fukai S, Horiuchi H. A SNARE geranylgeranyltransferase essential for the organization of the Golgi apparatus. EMBO J 39, e104120, 2020. 10. Schäfer J, Schessner JP, Tsuju T, Funaya C, Bircham PW, Schaeff K, Ruffini G, Fujimoto T, Schuck S. ESCRT machinery mediates selective microautophagy of endoplasmic reticulum. EMBO J 39, e102586, 2020. 11. Tsuji T, Cheng J, Tatematsu T, Ebata A, Kamikawa H, Fujita A, Gyobu S, Segawa K, Arai H, Taguchi T, Nagata S, Fujimoto T. Predominant localization of phosphatidylserine at the cytoplasmic leaflet of the ER, and its TMEM16K-dependent redistribution. Proc Natl Acad Sci USA, 116, 13368-13373, 2019. 12. Sołtysik K, Ohsaki Y, Tatematsu T, Cheng J, Fujimoto T. Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis Nat Commun 10, 473, 2019. doi: 10.1038/s41467-019-08411-x 13. Yamaguchi T, Hayashi M, Ida L, Yamamoto M, Lu C, Kajino T, Cheng J, Nakatochi M, Isomura H, Suzuki M, Fujimoto T, Takahashi T. ROR1–CAVIN3 interaction is required for caveolae-dependent endocytosis and prosurvival signaling in lung adenocarcinoma. Oncogene 35, 5142-5157, 2019. 14. Kawai T, Miyata H, Nakanishi H, Sakata S, Morioka S, Sasaki J, Watanabe M, Sakimura K, Fujimoto T, Sasaki T, Ikawa M, Okamura Y. Polarized PtdIns(4,5)P2 distribution mediated by a voltage-sensing phosphatase (VSP) regulates sperm motility. Proc Natl Acad Sci USA 116, 26020-26026, 2019. 15. Sobol M, Kalendová A, Yildirim S, Kalasová I, Fáberová V, Vrkoslav V, Philimonenko V, Marášek P, Pastorek L, Čapek M, Lubovská Z, Uličná L, Tsuji T, Lísa M, Cvačka J, Fujimoto T, Hozak P. Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription. J Cell Sci 131, jcs211094, 2018. doi: 10.1242/jcs.211094 16. Kimura H, Arasaki K, Ohsaki Y, Fujimoto T, Tagaya M. Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3. J Lipid Res 59, 805-819, 2018. 17. Yang L, Wang L, Ma J, Yang G, Cui S, Geng T, Mordue D, Fujimoto T, Cheng G, You F, Lin R, Fikrig E, Wang P. UBXN3B positively regulates STING mediated antiviral immune responses. Nat Commun 9, 2329, 2018. 18. Sugo M, Kimura H, Arasaki K, Amemiya T, Hirota N, Dohmae N, Imai Y, Inoshita T, Shiba-Fukushima K, Hattori N, Fujimoto T, Wakana Y, Inoue H, Tagaya M. Syntaxin 17 regulates the localization and function of PGAM5 in mitochondrial division and mitophagy. EMBO J 37, e98899, 2018. doi: 10.15252/embj.201798899 19. Imai N, Suzuki M, Ishizu Y, Honda T, Hayashi K, Ishigami M, Hirooka Y, Ishihara T, Goto H, Fujimoto T. Hepatocyte-specific depletion of ubiquitin regulatory X domain containing protein 8 (UBXD8) accelerates fibrosis in a mouse non-alcoholic steatohepatitis model. Histochem Cell Biol 148, 219-227, 2017. doi: 10.1007/s00418-017-1572-6 20. Tsuji T, Fujimoto M, Tatematsu T, Cheng J, Orii M, Takatori S, Fujimoto T. Niemann-Pick type C proteins promote microautophagy by expanding raft-like membrane domains in the yeast vacuole. eLife 6, e25960, 2017. doi: 10.7554/eLife.25960 21. Oku M, Maeda Y, Kagohashi Y, Kondo T, Yamada M, Fujimoto T, Sakai Y. Evidence for ESCRT- and clathrin-dependent microautophagy. J Cell Biol 216, 3263-3274, 2017. doi: 10.1083/jcb.201611029 22. Tsuji T, Fujimoto T. Freeze-fracture-etching electron microscopy for facile analysis of yeast ultrastructure. Bio Protoc 7, e2556, 2017. 23. Aktar S, Takatori S, Tsuji T, Ohsaki Y, Cheng J, Fujimoto T. A new electron microscopic method to observe the distribution of phosphatidylinositol 3,4-bisphosphate. Acta Histochem Cytochem 50, 141-147, 2017. 24. Yamaguchi T, Lu C, Yanagisawa K, Can Lu, Usukura J, Cheng J, Hotta N, Shimada Y, Ida R, Isomura H, Suzuki M, Fujimoto T, Takahashi T. ROR1 sustains caveolae and survival signaling as a scaffold of cavin-1 and caveolin-1. Nat Commun 7, 10060, 2016. doi: 10.1038/ncomms10060 25. Takatori S, Tatematsu T, Cheng J, Matsumoto J, Akano T, Fujimoto T. Phosphatidylinositol 3,5-bisphosphate-rich membrane domains in endosomes and lysosomes. Traffic 17, 154-167, 2016. 26. Ohsaki Y, Kawai T, Yoshikawa Y, Cheng J, Jokitalo E, Fujimoto T. PML isoform II plays a critical role in nuclear lipid droplet formation. J Cell Biol 212, 29-38, 2016. 27. Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton-Vandenbroucke I, Kobayashi T. Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol Biol Cell 27, 3293-3304, 2016. 28. Takabe W, Urano Y, Vo D-K H, Shibuya K, Tanno M, Kitagishi H, Fujimoto T, Noguchi N. Esterification of 24(S)-hydroxycholesterol induces formation of atypical lipid droplet-like structures, leading to neuronal cell death. J Lipid Res 57, 2005-2014, 2016. 29. Suzuki M, Murakami T, Cheng J, Kano H, Fukata M, Fujimoto T. ELMOD2 is anchored to lipid droplets by palmitoylation and regulates ATGL recruitment. Mol Biol Cell 26, 2333-2342, 2015. 30. Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih P-J, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T. Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay. Sci Rep 5, 11333, 2015. doi:10.1038/srep11333, 2015 31. Yamauchi Y, Iwamoto N, Rogers MA, Abe-Dohmae S, Fujimoto T, Chang CC, Ishigami M, Kishimoto T, Kobayashi T, Ueda K, Furukawa K, Chang TY, and Yokoyama S. Deficiency in the lipid exporter ABCA1 impairs retrograde sterol movement and disrupts sterol sensing at the endoplasmic reticulum. J Biol Chem 290, 23464-77, 2015. doi: 10.1074/jbc.M115.662668 32. Imai N, Suzuki M, Hayashi K, Ishigami M, Hirooka Y, Abe T, Shioi G, Goto H, Fujimoto T. ( co-first authors) Hepatocyte-specific depletion of UBXD8 induces periportal steatosis in mice fed a high-fat diet. PLoS One 10, e0127114, 2015. doi:10.1371/journal.pone.0127114 33. Kagawa Y, Yasumoto Y, Sharifi K, Ebrahimi M, Islam A, Miyazaki H, Yamamoto Y, Sawada T, Tokuda N, Kobayashi S, Maekawa M, Yoshikawa T, Takaki E, Nakai A, Kogo H, Fujimoto T, Owada Y. Fatty acid-binding protein 7 regulates function of caveolae in astrocytes through expression of caveolin-1. Glia 63, 780-794, 2015. doi: 10.1002/glia.22784 34. Arasaki K, Shimizu H, Mogari H, Nishida N, Hirota N, Furuno A, Kudo Y, Baba M, Baba N, Cheng J, Fujimoto T, Ishihara N, Ortiz-Sandoval C, Barlow L, Raturi A, Dohmae N, Wakana Y, Inoue H, Tani K, Dacks J, Simmen T, Tagaya M. A Role for the Ancient SNARE Syntaxin 17 in Regulating Mitochondrial Division. Dev Cell 32, 304-317, 2015. （総説） 1. Fujimoto T*. Nuclear lipid droplets—how are they different from their cytoplasmic siblings? J Cell Sci 135, jcs259253, 2022. 2. Ogasawara Y, Tsuji T, Fujimoto T. Multifarious roles of lipid droplets in autophagy – Target, product, and what else? Semin Cell Dev Biol 108, 47-54, 2020. 3. Yamaguchi T, Fujimoto T, Takahashi T. Method for efficient observation of caveolin-1 in plasma membrane by microscopy imaging analysis. Methods Mol Biol 2169, 43-52, 2020. 4. Sołtysik K, Ohsaki Y, Fujimoto T. Duo in a mystical realm – nuclear lipid droplets and the inner nuclear membrane. Contact 2, 1-11, 2019. 5. Tsuji T, Takatori S, Fujimoto T. Definition of phosphoinositide distribution in the nanoscale. Curr Op Cell Biol 57, 33-39, 2019. 6. Tsuji T, Fujimoto T. Lipids and lipid domains in the yeast vacuole. Biochem Soc Trans 46, 1047-1054, 2018. 7. Tsuji T, Fujita A, Fujimoto T. Immunoelectron microscopy of gangliosides. Methods Mol Biol 1804, 231-239, 2018. 8. Ohsaki Y, Sołtysik K, Fujimoto, T. The lipid droplet and the endoplasmic reticulum. Adv Exp Med Biol 997, 111-120, 2017. 9. Fujimoto T, Parmryd, I. Interleaflet coupling, pinning and leaflet asymmetry – major players in plasma membrane nanodomain formation. Frontier Cell Dev Biol 4, 155, 2017. doi: 10.3389/fcell.2016.00155 10. Takatori S, Fujimoto T. A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane. Commun Integr Biol 9, e1145319, 2016. doi: 10.1080/19420889.2016.1145319 11. Takatori S, Fujimoto T. Microscopy of membrane lipids - how precisely can we define their distribution? Essays in Biochemistry 57, 81-91, 2015.

研究テーマ

膜脂質、膜ドメイン、脂肪滴、電子顕微鏡

研究業績（論文）

（原著論文）
1. Osakada H, Fujimoto T*. On-grid labeling method for freeze-fracture replicas. Microscopy 72, 56, 2023.
2. Mioka T, Tian G, Wang S, Tsuji T, Kishimoto T, Fujimoto T, *Tanaka K. A micron-scale protein-depleted plasma membrane domain could be generated in phosphatidylserine-deficient yeast cells. J Cell Sci 135, jcs256529, 2022.
3. Tsuji T, Fujimoto, T*. Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy. STAR Protocol 2, 100990, 2021.
4. Soltysik K, Ohsaki Y, Tatematsu T, Cheng J, Maeda A, Morita S, Fujimoto T*. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner. J Cell Biol 220, e202005026, 2021.
5. Orii M, Tsuji T, Ogasawara Y, Fujimoto T*. Transmembrane phospholipid translocation mediated by Atg9 is involved in autophagosome formation. J Cell Biol 220, e202009194, 2021.
6. Matoba K, Kotani T, Tsutsumi A, Tsuji T, Mori T, Noshiro D, Sugita Y, Nomura N, Iwata S, Ohsumi Y, Fujimoto T, Nakatogawa H, Kikkawa M, *Noda NN. A lipid scramblase mediates autophagosomal membrane expansion. Nat Struct Mol Biol 27, 1185-1193, 2020.
7. Ogasawara Y, Cheng J, Tatematsu T, Uchida M, Murase O, Yoshikawa S, Ohsaki Y, *Fujimoto T. Long-term autophagy is sustained by activation of CCTbeta3 on lipid droplets. Nat Commun 11, 4480, 2020.
8. Masuda T, Hirose H, Baba K, Walrant A, Sagan S, Inagaki N, Fujimoto T, Futaki S. An artificial amphiphilic peptide promotes endocytic uptake by inducing membrane curvature. Bioconjug Chem 31, 1611-1615, 2020. 10.1021/acs.bioconjchem.0c00239.
9. Shirakawa R, Goto-Ito S, Goto K, Wakayama S, Kubo H, Sakata N, Trinh DA, Yamagata A, Sato Y, Masumoto H, Cheng J, Fujimoto T, Fukai S, Horiuchi H. A SNARE geranylgeranyltransferase essential for the organization of the Golgi apparatus. EMBO J 39, e104120, 2020.
10. Schäfer J, Schessner JP, Tsuju T, Funaya C, Bircham PW, Schaeff K, Ruffini G, Fujimoto T, Schuck S. ESCRT machinery mediates selective microautophagy of endoplasmic reticulum. EMBO J 39, e102586, 2020.
11. Tsuji T, Cheng J, Tatematsu T, Ebata A, Kamikawa H, Fujita A, Gyobu S, Segawa K, Arai H, Taguchi T, Nagata S, Fujimoto T. Predominant localization of phosphatidylserine at the cytoplasmic leaflet of the ER, and its TMEM16K-dependent redistribution. Proc Natl Acad Sci USA, 116, 13368-13373, 2019.
12. Sołtysik K, Ohsaki Y, Tatematsu T, Cheng J, Fujimoto T. Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis Nat Commun 10, 473, 2019. doi: 10.1038/s41467-019-08411-x
13. Yamaguchi T, Hayashi M, Ida L, Yamamoto M, Lu C, Kajino T, Cheng J, Nakatochi M, Isomura H, Suzuki M, Fujimoto T, Takahashi T. ROR1–CAVIN3 interaction is required for caveolae-dependent endocytosis and prosurvival signaling in lung adenocarcinoma. Oncogene 35, 5142-5157, 2019.
14. Kawai T, Miyata H, Nakanishi H, Sakata S, Morioka S, Sasaki J, Watanabe M, Sakimura K, Fujimoto T, Sasaki T, Ikawa M, Okamura Y. Polarized PtdIns(4,5)P2 distribution mediated by a voltage-sensing phosphatase (VSP) regulates sperm motility. Proc Natl Acad Sci USA 116, 26020-26026, 2019.
15. Sobol M, Kalendová A, Yildirim S, Kalasová I, Fáberová V, Vrkoslav V, Philimonenko V, Marášek P, Pastorek L, Čapek M, Lubovská Z, Uličná L, Tsuji T, Lísa M, Cvačka J, Fujimoto T, Hozak P. Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription. J Cell Sci 131, jcs211094, 2018. doi: 10.1242/jcs.211094
16. Kimura H, Arasaki K, Ohsaki Y, Fujimoto T, Tagaya M. Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3. J Lipid Res 59, 805-819, 2018.
17. Yang L, Wang L, Ma J, Yang G, Cui S, Geng T, Mordue D, Fujimoto T, Cheng G, You F, Lin R, Fikrig E, Wang P. UBXN3B positively regulates STING mediated antiviral immune responses. Nat Commun 9, 2329, 2018.
18. Sugo M, Kimura H, Arasaki K, Amemiya T, Hirota N, Dohmae N, Imai Y, Inoshita T, Shiba-Fukushima K, Hattori N, Fujimoto T, Wakana Y, Inoue H, Tagaya M. Syntaxin 17 regulates the localization and function of PGAM5 in mitochondrial division and mitophagy. EMBO J 37, e98899, 2018. doi: 10.15252/embj.201798899
19. Imai N, Suzuki M, Ishizu Y, Honda T, Hayashi K, Ishigami M, Hirooka Y, Ishihara T, Goto H, Fujimoto T. Hepatocyte-specific depletion of ubiquitin regulatory X domain containing protein 8 (UBXD8) accelerates fibrosis in a mouse non-alcoholic steatohepatitis model. Histochem Cell Biol 148, 219-227, 2017. doi: 10.1007/s00418-017-1572-6
20. Tsuji T, Fujimoto M, Tatematsu T, Cheng J, Orii M, Takatori S, Fujimoto T. Niemann-Pick type C proteins promote microautophagy by expanding raft-like membrane domains in the yeast vacuole. eLife 6, e25960, 2017. doi: 10.7554/eLife.25960
21. Oku M, Maeda Y, Kagohashi Y, Kondo T, Yamada M, Fujimoto T, Sakai Y. Evidence for ESCRT- and clathrin-dependent microautophagy. J Cell Biol 216, 3263-3274, 2017. doi: 10.1083/jcb.201611029
22. Tsuji T, Fujimoto T. Freeze-fracture-etching electron microscopy for facile analysis of yeast ultrastructure. Bio Protoc 7, e2556, 2017.
23. Aktar S, Takatori S, Tsuji T, Ohsaki Y, Cheng J, Fujimoto T. A new electron microscopic method to observe the distribution of phosphatidylinositol 3,4-bisphosphate. Acta Histochem Cytochem 50, 141-147, 2017.
24. Yamaguchi T, Lu C, Yanagisawa K, Can Lu, Usukura J, Cheng J, Hotta N, Shimada Y, Ida R, Isomura H, Suzuki M, Fujimoto T, Takahashi T. ROR1 sustains caveolae and survival signaling as a scaffold of cavin-1 and caveolin-1. Nat Commun 7, 10060, 2016. doi: 10.1038/ncomms10060
25. Takatori S, Tatematsu T, Cheng J, Matsumoto J, Akano T, Fujimoto T. Phosphatidylinositol 3,5-bisphosphate-rich membrane domains in endosomes and lysosomes. Traffic 17, 154-167, 2016.
26. Ohsaki Y, Kawai T, Yoshikawa Y, Cheng J, Jokitalo E, Fujimoto T. PML isoform II plays a critical role in nuclear lipid droplet formation. J Cell Biol 212, 29-38, 2016.
27. Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton-Vandenbroucke I, Kobayashi T. Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol Biol Cell 27, 3293-3304, 2016.
28. Takabe W, Urano Y, Vo D-K H, Shibuya K, Tanno M, Kitagishi H, Fujimoto T, Noguchi N. Esterification of 24(S)-hydroxycholesterol induces formation of atypical lipid droplet-like structures, leading to neuronal cell death. J Lipid Res 57, 2005-2014, 2016.
29. Suzuki M, Murakami T, Cheng J, Kano H, Fukata M, Fujimoto T. ELMOD2 is anchored to lipid droplets by palmitoylation and regulates ATGL recruitment. Mol Biol Cell 26, 2333-2342, 2015.
30. Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih P-J, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T. Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay. Sci Rep 5, 11333, 2015. doi:10.1038/srep11333, 2015
31. Yamauchi Y, Iwamoto N, Rogers MA, Abe-Dohmae S, Fujimoto T, Chang CC, Ishigami M, Kishimoto T, Kobayashi T, Ueda K, Furukawa K, Chang TY, and Yokoyama S. Deficiency in the lipid exporter ABCA1 impairs retrograde sterol movement and disrupts sterol sensing at the endoplasmic reticulum. J Biol Chem 290, 23464-77, 2015. doi: 10.1074/jbc.M115.662668
32. Imai N*, Suzuki M*, Hayashi K, Ishigami M, Hirooka Y, Abe T, Shioi G, Goto H, Fujimoto T. (* co-first authors) Hepatocyte-specific depletion of UBXD8 induces periportal steatosis in mice fed a high-fat diet. PLoS One 10, e0127114, 2015. doi:10.1371/journal.pone.0127114
33. Kagawa Y, Yasumoto Y, Sharifi K, Ebrahimi M, Islam A, Miyazaki H, Yamamoto Y, Sawada T, Tokuda N, Kobayashi S, Maekawa M, Yoshikawa T, Takaki E, Nakai A, Kogo H, Fujimoto T, Owada Y. Fatty acid-binding protein 7 regulates function of caveolae in astrocytes through expression of caveolin-1. Glia 63, 780-794, 2015. doi: 10.1002/glia.22784
34. Arasaki K, Shimizu H, Mogari H, Nishida N, Hirota N, Furuno A, Kudo Y, Baba M, Baba N, Cheng J, Fujimoto T, Ishihara N, Ortiz-Sandoval C, Barlow L, Raturi A, Dohmae N, Wakana Y, Inoue H, Tani K, Dacks J, Simmen T, Tagaya M. A Role for the Ancient SNARE Syntaxin 17 in Regulating Mitochondrial Division. Dev Cell 32, 304-317, 2015.

（総説）
1. Fujimoto T*. Nuclear lipid droplets—how are they different from their cytoplasmic siblings? J Cell Sci 135, jcs259253, 2022.
2. Ogasawara Y, Tsuji T, Fujimoto T. Multifarious roles of lipid droplets in autophagy – Target, product, and what else? Semin Cell Dev Biol 108, 47-54, 2020.
3. Yamaguchi T, Fujimoto T, Takahashi T. Method for efficient observation of caveolin-1 in plasma membrane by microscopy imaging analysis. Methods Mol Biol 2169, 43-52, 2020.
4. Sołtysik K, Ohsaki Y, Fujimoto T. Duo in a mystical realm – nuclear lipid droplets and the inner nuclear membrane. Contact 2, 1-11, 2019.
5. Tsuji T, Takatori S, Fujimoto T. Definition of phosphoinositide distribution in the nanoscale. Curr Op Cell Biol 57, 33-39, 2019.
6. Tsuji T, Fujimoto T. Lipids and lipid domains in the yeast vacuole. Biochem Soc Trans 46, 1047-1054, 2018.
7. Tsuji T, Fujita A, Fujimoto T. Immunoelectron microscopy of gangliosides. Methods Mol Biol 1804, 231-239, 2018.
8. Ohsaki Y, Sołtysik K, Fujimoto, T. The lipid droplet and the endoplasmic reticulum. Adv Exp Med Biol 997, 111-120, 2017.
9. Fujimoto T, Parmryd, I. Interleaflet coupling, pinning and leaflet asymmetry – major players in plasma membrane nanodomain formation. Frontier Cell Dev Biol 4, 155, 2017. doi: 10.3389/fcell.2016.00155
10. Takatori S, Fujimoto T. A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane. Commun Integr Biol 9, e1145319, 2016. doi: 10.1080/19420889.2016.1145319
11. Takatori S, Fujimoto T. Microscopy of membrane lipids - how precisely can we define their distribution? Essays in Biochemistry 57, 81-91, 2015.

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【学校法人順天堂】研究者情報データベース

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藤本 豊士（フジモト トヨシ）

藤本豊士（フジモトトヨシ）