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研究業績詳細

安川武宏（ヤスカワタケヒロ）

研究テーマ	がん生物学、ミトコンドリアDNA、ミトコンドリア病
研究業績（論文）	【英語原著論文等】 1. Koyama, Y., Okazaki, H., Shi, Y., Mezawa, Y., Wang, Z., Sakimoto, M., Ishizuka, A., Ito, Y., Koyama, T., Daigo, Y., Takano, A., Miyagi, Y., Yokose, T., Yamashita, T., Sugahara, K., Hino, H., Yang, L., Maruyama, R., Katakura, A., Yasukawa, T., Orimo, A. (2023) Increased RUNX3 expression mediates tumor-promoting ability of human breast cancer-associated fibroblasts. Cancer Med., 12(17), 18062-18077.【corresponding author】 2. Tani, H., Ishikawa, K., Tamashiro, H., Ogasawara, E., Yasukawa, T., Matsuda, S., Shimizu, A., Kang, D., Hayashi, J.I., Wei, F.Y., Nakada, K. (2022) Aberrant RNA processing contributes to the pathogenesis of mitochondrial diseases in trans-mitochondrial mouse model carrying mitochondrial tRNALeu(UUR) with a pathogenic A2748G mutation. Nucleic Acids Res. 50(16), 9382-9396. 3. Inatomi, T., Matsuda, S., Ishiuchi, T., Do, Y., Nakayama, M., Abe, S., Kasho, K., Wanrooij, S., Nakada, K., Ichiyanagi, K., Sasaki, H., Yasukawa, T., Kang, D. (2022) TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. Biochim. Biophys. Acta - Molecular Cell Research 1869(1), 119167. 【corresponding author】 4. Nomiyama, T., Setoyama, D., Yasukawa, T., Kang, D. (2022) Mitochondria metabolomics reveals a role of β-nicotinamide mononucleotide metabolism in mitochondrial DNA replication. J. Biochem. 171(3) 325-338. 5. Doi, G., Okada, S., Yasukawa, T., Sugiyama, Y., Bala, S., Miyazaki, S., Kang, D., Ito, T. (2021) Catalytically Inactive Cas9 Impairs DNA Replication Fork Progression to Induce Focal Genomic Instability. Nucleic Acids Research 49(2), 954-968. 6. Do, Y., Matsuda, S., Inatomi, T., Nakada, K., Yasukawa, T., Kang, D. (2020). The accessory subunit of human DNA polymerase γ is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit. Mitochondrion 53, 133-139 【corresponding author】 7. Fang, Y., Akimoto, M., Mayanagi, K., Hatano, A., Matsumoto, M., Matsuda, S., Yasukawa, T., Kang, D. (2020). Chemical acetylation of mitochondrial transcription factor A occurs on specific lysine residues and affects its ability to change global DNA topology. Mitochondrion 53, 99-108 【corresponding author】 8. Matsuda, S., Yasukawa, T., Sakaguchi, Y., Ichiyanagi, K., Unoki, M., Gotoh, K., Fukuda, K., Sasaki, H., Suzuki, T., Kang, D. (2018). Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA. Sci. Rep. 8(1):5801 【corresponding author】 9. Mezuki, S., Fukuda, K., Matsushita, T., Fukushima, Y., Matsuo, R., Goto, Y.I., Yasukawa, T., Uchiumi, T., Kang, D., Kitazono, T., Ago, T. (2017). Isolated and repeated stroke-like episodes in a middle-aged man with a mitochondrial ND3 T10158C mutation: a case report. BMC Neurol. 17(1): 217. 10. Moss, C.F., Dalla Rosa, I., Hunt, L.E., Yasukawa, T., Young, R., Jones, A.W.E., Reddy, K., Desai, R., Virtue, S., Elgar, G., Voshol, P., Taylor, M.S., Holt, I.J., Reijns, M.A.M., Spinazzola, A. (2017). Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model. Nucleic Acids Res. 45(22), 12808-12815. 11. Fakruddin, M., Wei, F.-Y., Emura, S., Matsuda, S., Yasukawa, T., Kang, D., Tomizawa, K. (2017). Cdk5rap1-mediated 2-methylthio-N6-isopentenyladenosine modification is absent from nuclear-derived RNA species. Nucleic Acids Res. 45(20), 11954-11961. 12. Akman, G., Desai, R., Bailey, L.J., Yasukawa, T., Dalla Rosa, I., Durigon, R., Holmes, J.B., Moss, C.F., Mennuni, M., Houlden, H., Crouch, R.J., Hanna, M.G., Pitceathly, R.D., Spinazzola, A., Holt, I.J. (2016). Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. Proc. Natl. Acad. Sci. USA. 113(30), E4276-4285. 13. Qu, J., Yasukawa, T., Kang, D. (2016). Suppression of mitochondrial transcription initiation complexes changes the balance of replication intermediates of mitochondrial DNA and reduces 7S DNA in cultured human cells. J. Biochem. 160(1), 49-57. 【corresponding author】（2017年日本生化学会JB論文賞受賞論文） 14. Zhang, H., Zhang, Y.W., Yasukawa, T., Dalla Rosa, I., Khiati, S., Pommier, Y. (2014). Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases IIα and IIβ in vertebrate mitochondria, Nucleic Acids Res. 42(11), 7259-7267. 15. Reyes, A., Kazak, L., Wood, S.R., Yasukawa, T., Jacobs, H.T., Holt I.J. (2013). Mitochondrial DNA replication proceeds via a ‘bootlace’ mechanism involving the incorporation of processed transcripts, Nucleic Acids Res. 41(11), 5837-5850. 16. Yao Z., Jones A. W. E., Fassone E., Sweeney M. G., Lebiedzinska M., Suski J. M., Wieckowski M. R., Tajeddine N., Hargreaves I. P., Yasukawa T., Tufo G., Brenner C., Kroemer G., Rahman S., Szabadkai G. (2013). Oncogene 32(20), 2592-2600. 17. Ruhanen H., Ushakov K., Yasukawa, T. (2011). Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells. Biochim. Biophys. Acta - Molecular Cell Research 1813(12), 2000-2007. 【corresponding author】 18. Ruhanen, H., Borrie, S., Szabadkai, G., Tyynismaa, H., Jones, A. W. E., Kang, D., Taanman, J.-W., Yasukawa, T. (2010). Mitochondrial single-stranded DNA binding protein is required for maintenance of mitochondrial DNA and 7S DNA but is not required for mitochondrial nucleoid organization. Biochim. Biophys. Acta - Molecular Cell Research 1803(8), 931-939. 【corresponding author】 19. Pohjoismäki, J. L. O., Holmes, J. B., Wood, S. R., Yang, M. Y., Yasukawa, T., Reyes, A., Bailey, L. J., Cluett, T. J., Goffart, S., Willcox, S., Rigby, R. E., Jackson, A. P., Spelbrink, J. N., Griffith, J. D., Crouch, R. J., Jacobs, H. T., Holt, I. J. (2010). Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid. J. Mol. Biol. 397(5), 1144-1155. 20. Di Re, M., Sembongi, H., He, J., Reyes, A., Yasukawa, T., Martinsson, P., Bailey, L. J., Goffart, S., Boyd-Kirkup, J. D., Wong, T. S., Fersht, A. R., Spelbrink, J. N., Holt, I. J. (2009). The accessory subunit of mitochondrial DNA polymerase gamma determines the DNA content of mitochondrial nucleoids in human cultured cells. Nucleic Acids Res. 37(17), 5701-5713. 21. Hyvärinen, A. K., Pohjoismäki, J. L. O., Reyes, A., Wanrooij, S., Yasukawa, T., Karhunen, P. J., Spelbrink, J. N., Holt, I. J., Jacobs, H. T. (2007). The mitochondrial transcription termination factor mTERF modulates replication pausing in human mitochondrial DNA. Nucleic Acids Res. 35(19), 6458-6474. 22. Wanrooij, S., Goffart, S., Pohjoismäki, J. L. O., Yasukawa, T., Spelbrink, J. N. (2007). Expression of catalytic mutants of the mtDNA helicase Twinkle and polymerase POLG causes distinct replication stalling phenotypes. Nucleic Acids Res. 35(10), 3238-3251. 23. Yasukawa, T., Reyes, A., Cluett, T. J., Yang, M. Y., Bowmaker, M., Jacobs, H. T., Holt, I. J. (2006). Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand. EMBO J. 25(22), 5358-5371. 24. Kirino, Y., Yasukawa, T., Marjavaara, S. K., Jacobs, H. T., Holt, I. J., Watanabe, K., Suzuki, T. (2006). Acquisition of the wobble modification in mitochondrial tRNALeu(CUN) bearing the G12300A mutation suppresses the MELAS molecular defect. Hum. Mol. Genet. 15(6), 897-904. 25. Yasukawa, T., Yang, M. Y., Jacobs, H. T., Holt, I. J. (2005). A bidirectional origin of replication maps to the major noncoding region of human mitochondrial DNA. Mol. Cell 18(6), 651-662. 26. Yasukawa, T., Kirino, Y., Ishii, N., Holt, I. J., Jacobs, H. T., Makifuchi, T., Fukuhara, N., Ohta, S., Suzuki, T., Watanabe, K. (2005). Wobble modification deficiency in mutant tRNAs in patients with mitochondrial diseases. FEBS Lett. 579(13), 2948-2952. 27. Kirino, Y., Yasukawa, T., Ohta, S., Akira, S., Ishihara, K., Watanabe, K., Suzuki, T. (2004). Codon-specific translational defect caused by a wobble modification deficiency in mutant tRNA from a human mitochondrial disease. Proc. Natl. Acad. Sci. USA. 101(42), 15070-15075. 28. Hino, N., Suzuki, T., Yasukawa, T., Seio, K., Watanabe, K., Ueda, T. (2004). The pathogenic A4269G mutation in human mitochondrial tRNA(Ile) alters the T-stem structure and decreases the binding affinity for elongation factor Tu. Genes Cells 9(3), 243-252. 29. Bowmaker, M., Yang, M. Y., Yasukawa, T., Reyes, A., Jacobs, H. T., Huberman, J. A., Holt, I. J. (2003). Mammalian mitochondrial DNA replicates bidirectionally from an initiation zone. J. Biol. Chem. 278(51), 50961-50969. 30. Toompuu, M., Yasukawa, T., Suzuki, T., Hakkinen, T., Spelbrink, J. N., Watanabe, K., Jacobs, H. T. (2002). The 7472insC mitochondrial DNA mutation impairs the synthesis and extent of aminoacylation of tRNASer(UCN) but not its structure or rate of turnover. J. Biol. Chem. 277(25), 22240-22250. 31. Yasukawa, T., Suzuki, T., Ishii, N., Ohta, S., Watanabe, K. (2001). Wobble modification defect in tRNA disturbs codon-anticodon interaction in a mitochondrial disease. EMBO J. 20(17), 4794-4802. 32. Yasukawa, T., Hino, N., Suzuki, T., Watanabe, K., Ueda, T., Ohta, S. (2000). A pathogenic point mutation reduces stability of mitochondrial mutant tRNA(Ile). Nucleic Acids Res. 28(19), 3779-3784. 33. Yasukawa, T., Suzuki, T., Ishii, N., Ueda, T., Ohta, S., Watanabe, K. (2000). Defect in modification at the anticodon wobble nucleotide of mitochondrial tRNA(Lys) with the MERRF encephalomyopathy pathogenic mutation. FEBS Lett. 467(2-3), 175-178. 34. Yasukawa, T., Suzuki, T., Suzuki, T., Ueda, T., Ohta, S., Watanabe, K. (2000). Modification defect at anticodon wobble nucleotide of mitochondrial tRNAs(Leu)(UUR) with pathogenic mutations of mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes. J. Biol. Chem. 275(6), 4251-4257. 【英語総説・著書など】 35. Yasukawa, T., Kang D. (2023). Assessing TFAM binding to human mitochondrial DNA. In Methods in Molecular Biology (Vol. 2615 pp.139-151). 【corresponding author】 36. Yasukawa, T., Matsuda, S., Kang D. (2020). Epigenetic features of mitochondrial DNA (Chapter 3, pp. 71-85). In The Human Mitochondrial Genome. Gasparre, G., Porcelli, A. M. (eds.). Academic Press. (Cambridge, USA). 【corresponding author】 37. Yasukawa, T., Kang D. (2018). An overview of mammalian mitochondrial DNA replication mechanisms. J. Biochem. 164(3), 183-193 【*corresponding author】 38. Yasukawa, T., Poulton, J. (2009). Mitochondrial DNA Replication. In Molecular Themes in DNA Replication. Cox, L. S. (ed.). (pp.316-345). London: Royal Society of Chemistry. 39. Reyes, A., Yasukawa, T., Cluett, T. J., Holt, I. J. (2009). Analysis of mitochondrial DNA by two-dimensional agarose gel electrophoresis. In Methods in Molecular Biology (Vol. 554 pp.15-35). 40. Reyes, A., Yasukawa, T., Holt, I. J. (2007). Analysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis. In Methods in Molecular Biology (Vol. 372 pp.219-232). 41. Kirino, Y., Yasukawa, T., Suzuki, T., Suzuki, T., Watanabe, K. (2003). Molecular mechanism of MELAS caused by taurine-modification defect in mitochondrial tRNA. In Korczyn, A. D. (Ed.) from Third International Congress on Vascular Dementia (pp.69-73). 42. Yasukawa, T., Suzuki, T., Ohta, S., Watanabe, K. (2002). Wobble modification defect suppresses translational activity of tRNAs with MERRF and MELAS mutations. Mitochondrion 2(1-2), 129-141. 43. Yasukawa, T., Suzuki, T., Ueda, T., Hayashi, J., Ohta, S., Watanabe, K. (1998). Stability of mitochondrial tRNA molecules with pathogenic point mutations. Nucleic Acids Symposium Series (Vol. 39 pp.257-258). 【日本語総説・著書など】 44. 安川武宏、康東天（2022）　「ミトコンドリアDNAの複製・維持機構と関連疾患」　医学のあゆみ　281(12), 1121-1127. 医歯薬出版 45. 安川武宏（2021）「ミトコンドリアゲノムの複製・維持」　ミトコンドリアダイナミクス　第４章第３節　pp121-129. ISBN 978-4-86043-746-6 C3045（株）エヌ・ティー・エス（東京） 46. 安川武宏、康東天（2019）　「ミトコンドリアゲノムの複製・維持機構」　実験医学　37(12), 24-30. 羊土社 47. 安川武宏、康東天（2018）　「ミトコンドリアDNAの維持システム Up-to-Date」メディカル・サイエンス・ダイジェスト　44(10), 17-20.　ニューサイエンス社 48. 安川武宏（2017）　「ミトコンドリアDNAのユニークな複製メカニズム」　医学のあゆみ　260(1), 5-10.　医歯薬出版 49. 太田成男、安川武宏（2012）「ミトコンドリアDNAの構造と発現制御」ミトコンドリア病－ up to date　月刊　臨床神経科学　30(9), 988-991. 中外医学社 50. 安川武宏（2010）「海外研究生活のすすめ」　せるてく・あらかると　細胞工学　vol. 29, No. 5, 474-476.　学研メディカル秀潤社 51. 安川武宏（2009）「国際色豊かな環境で」　ラボレポート―独立編　実験医学　vol. 27, No. 18, 3040-3042. 羊土社 52. 安川武宏（2005）「ミトコンドリアDNAのユニークな複製機構と転写機構」　タンパク質核酸酵素　50(14), 1727-1731.　共立出版 53. 安川武宏（2005）「ミトコンドリアDNAの複製メカニズム」　細胞工学　vol. 24, No. 8, 809-813. 秀潤社 54. 安川武宏（2004）「ミトコンドリアDNA複製機構のパラダイムシフト」　Molecular Medicine 41(3), 269-276. 中山書店 55. 安川武宏（2002）「ミトコンドリアDNAの複製機序と修復機構」　ミトコンドリアとミトコンドリア病　日本臨牀増刊号 60 (増刊号4), 24-29. 日本臨牀社 56. 安川武宏、鈴木勉、太田成男、渡辺公綱（2002）「変異ミトコンドリアtRNAの修飾欠損とdecoding異常」　ミトコンドリアとミトコンドリア病　日本臨牀増刊号　60 (増刊号4), 197-201.　日本臨牀社 57. 安川武宏、太田成男（2001）「サイブリッドの作製法と大量培養法」in 新ミトコンドリア学. 内海・井上　監修、共立出版（東京）．pp.392-394. 58. 太田成男、安川武宏、上田卓也（2000）「ミトコンドリア病とtRNA」in RNA研究の最前線.志村・渡辺　共編、シュプリンガ－・フェアラーク東京（東京）．pp.188-200.　 59. 安川武宏、渡辺公綱、太田成男（2000）「ミトコンドリア病発症研究の新しいアプローチ―ミトコンドリアtRNAアンチコドンの異常」　化学と生物　38 (11), 714-721.学会出版センター 60. 太田成男、安川武宏（2000）「ミトコンドリア脳筋症―ミトコンドリア変異tRNAのwobbling異常」　細胞工学　19, 596-604.　秀潤社

研究テーマ

がん生物学、ミトコンドリアDNA、ミトコンドリア病

研究業績（論文）

【英語原著論文等】

1. Koyama, Y., Okazaki, H., Shi, Y., Mezawa, Y., Wang, Z., Sakimoto, M., Ishizuka, A., Ito, Y., Koyama, T., Daigo, Y., Takano, A., Miyagi, Y., Yokose, T., Yamashita, T., Sugahara, K., Hino, H., Yang, L., Maruyama, R., Katakura, A., Yasukawa, T.*, Orimo, A.* (2023) Increased RUNX3 expression mediates tumor-promoting ability of human breast cancer-associated fibroblasts. Cancer Med., 12(17), 18062-18077.【*corresponding author】

2. Tani, H., Ishikawa, K., Tamashiro, H., Ogasawara, E., Yasukawa, T., Matsuda, S., Shimizu, A., Kang, D., Hayashi, J.I., Wei, F.Y., Nakada, K. (2022) Aberrant RNA processing contributes to the pathogenesis of mitochondrial diseases in trans-mitochondrial mouse model carrying mitochondrial tRNALeu(UUR) with a pathogenic A2748G mutation. Nucleic Acids Res. 50(16), 9382-9396.

3. Inatomi, T., Matsuda, S., Ishiuchi, T., Do, Y., Nakayama, M., Abe, S., Kasho, K., Wanrooij, S., Nakada, K., Ichiyanagi, K., Sasaki, H., Yasukawa, T.*, Kang, D. (2022) TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. Biochim. Biophys. Acta - Molecular Cell Research 1869(1), 119167. 【*corresponding author】

4. Nomiyama, T., Setoyama, D., Yasukawa, T., Kang, D. (2022) Mitochondria metabolomics reveals a role of β-nicotinamide mononucleotide metabolism in mitochondrial DNA replication. J. Biochem. 171(3) 325-338.

5. Doi, G., Okada, S., Yasukawa, T., Sugiyama, Y., Bala, S., Miyazaki, S., Kang, D., Ito, T. (2021) Catalytically Inactive Cas9 Impairs DNA Replication Fork Progression to Induce Focal Genomic Instability. Nucleic Acids Research 49(2), 954-968.

6. Do, Y., Matsuda, S., Inatomi, T., Nakada, K., Yasukawa, T.*, Kang, D. (2020). The accessory subunit of human DNA polymerase γ is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit. Mitochondrion 53, 133-139 【*corresponding author】

7. Fang, Y., Akimoto, M., Mayanagi, K., Hatano, A., Matsumoto, M., Matsuda, S., Yasukawa, T.*, Kang, D. (2020). Chemical acetylation of mitochondrial transcription factor A occurs on specific lysine residues and affects its ability to change global DNA topology. Mitochondrion 53, 99-108 【*corresponding author】

8. Matsuda, S., Yasukawa, T.*, Sakaguchi, Y., Ichiyanagi, K., Unoki, M., Gotoh, K., Fukuda, K., Sasaki, H., Suzuki, T., Kang, D. (2018). Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA. Sci. Rep. 8(1):5801 【*corresponding author】

9. Mezuki, S., Fukuda, K., Matsushita, T., Fukushima, Y., Matsuo, R., Goto, Y.I., Yasukawa, T., Uchiumi, T., Kang, D., Kitazono, T., Ago, T. (2017). Isolated and repeated stroke-like episodes in a middle-aged man with a mitochondrial ND3 T10158C mutation: a case report. BMC Neurol. 17(1): 217.

10. Moss, C.F., Dalla Rosa, I., Hunt, L.E., Yasukawa, T., Young, R., Jones, A.W.E., Reddy, K., Desai, R., Virtue, S., Elgar, G., Voshol, P., Taylor, M.S., Holt, I.J., Reijns, M.A.M., Spinazzola, A. (2017). Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model. Nucleic Acids Res. 45(22), 12808-12815.

11. Fakruddin, M., Wei, F.-Y., Emura, S., Matsuda, S., Yasukawa, T., Kang, D., Tomizawa, K. (2017). Cdk5rap1-mediated 2-methylthio-N6-isopentenyladenosine modification is absent from nuclear-derived RNA species. Nucleic Acids Res. 45(20), 11954-11961.

12. Akman, G., Desai, R., Bailey, L.J., Yasukawa, T., Dalla Rosa, I., Durigon, R., Holmes, J.B., Moss, C.F., Mennuni, M., Houlden, H., Crouch, R.J., Hanna, M.G., Pitceathly, R.D., Spinazzola, A., Holt, I.J. (2016). Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. Proc. Natl. Acad. Sci. USA. 113(30), E4276-4285.

13. Qu, J., Yasukawa, T.*, Kang, D. (2016). Suppression of mitochondrial transcription initiation complexes changes the balance of replication intermediates of mitochondrial DNA and reduces 7S DNA in cultured human cells. J. Biochem. 160(1), 49-57. 【*corresponding author】（2017年日本生化学会JB論文賞受賞論文）

14. Zhang, H., Zhang, Y.W., Yasukawa, T., Dalla Rosa, I., Khiati, S., Pommier, Y. (2014). Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases IIα and IIβ in vertebrate mitochondria, Nucleic Acids Res. 42(11), 7259-7267.

15. Reyes, A., Kazak, L., Wood, S.R., Yasukawa, T., Jacobs, H.T., Holt I.J. (2013). Mitochondrial DNA replication proceeds via a ‘bootlace’ mechanism involving the incorporation of processed transcripts, Nucleic Acids Res. 41(11), 5837-5850.

16. Yao Z., Jones A. W. E., Fassone E., Sweeney M. G., Lebiedzinska M., Suski J. M., Wieckowski M. R., Tajeddine N., Hargreaves I. P., Yasukawa T., Tufo G., Brenner C., Kroemer G., Rahman S., Szabadkai G. (2013). Oncogene 32(20), 2592-2600.

17. Ruhanen H., Ushakov K., Yasukawa, T.* (2011). Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells. Biochim. Biophys. Acta - Molecular Cell Research 1813(12), 2000-2007. 【*corresponding author】

18. Ruhanen, H., Borrie, S., Szabadkai, G., Tyynismaa, H., Jones, A. W. E., Kang, D., Taanman, J.-W., Yasukawa, T.* (2010). Mitochondrial single-stranded DNA binding protein is required for maintenance of mitochondrial DNA and 7S DNA but is not required for mitochondrial nucleoid organization. Biochim. Biophys. Acta - Molecular Cell Research 1803(8), 931-939. 【*corresponding author】

19. Pohjoismäki, J. L. O., Holmes, J. B., Wood, S. R., Yang, M. Y., Yasukawa, T., Reyes, A., Bailey, L. J., Cluett, T. J., Goffart, S., Willcox, S., Rigby, R. E., Jackson, A. P., Spelbrink, J. N., Griffith, J. D., Crouch, R. J., Jacobs, H. T., Holt, I. J. (2010). Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid. J. Mol. Biol. 397(5), 1144-1155.

20. Di Re, M., Sembongi, H., He, J., Reyes, A., Yasukawa, T., Martinsson, P., Bailey, L. J., Goffart, S., Boyd-Kirkup, J. D., Wong, T. S., Fersht, A. R., Spelbrink, J. N., Holt, I. J. (2009). The accessory subunit of mitochondrial DNA polymerase gamma determines the DNA content of mitochondrial nucleoids in human cultured cells. Nucleic Acids Res. 37(17), 5701-5713.

21. Hyvärinen, A. K., Pohjoismäki, J. L. O., Reyes, A., Wanrooij, S., Yasukawa, T., Karhunen, P. J., Spelbrink, J. N., Holt, I. J., Jacobs, H. T. (2007). The mitochondrial transcription termination factor mTERF modulates replication pausing in human mitochondrial DNA. Nucleic Acids Res. 35(19), 6458-6474.

22. Wanrooij, S., Goffart, S., Pohjoismäki, J. L. O., Yasukawa, T., Spelbrink, J. N. (2007). Expression of catalytic mutants of the mtDNA helicase Twinkle and polymerase POLG causes distinct replication stalling phenotypes. Nucleic Acids Res. 35(10), 3238-3251.

23. Yasukawa, T., Reyes, A., Cluett, T. J., Yang, M. Y., Bowmaker, M., Jacobs, H. T., Holt, I. J. (2006). Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand. EMBO J. 25(22), 5358-5371.

24. Kirino, Y., Yasukawa, T., Marjavaara, S. K., Jacobs, H. T., Holt, I. J., Watanabe, K., Suzuki, T. (2006). Acquisition of the wobble modification in mitochondrial tRNALeu(CUN) bearing the G12300A mutation suppresses the MELAS molecular defect. Hum. Mol. Genet. 15(6), 897-904.

25. Yasukawa, T., Yang, M. Y., Jacobs, H. T., Holt, I. J. (2005). A bidirectional origin of replication maps to the major noncoding region of human mitochondrial DNA. Mol. Cell 18(6), 651-662.

26. Yasukawa, T., Kirino, Y., Ishii, N., Holt, I. J., Jacobs, H. T., Makifuchi, T., Fukuhara, N., Ohta, S., Suzuki, T., Watanabe, K. (2005). Wobble modification deficiency in mutant tRNAs in patients with mitochondrial diseases. FEBS Lett. 579(13), 2948-2952.

27. Kirino, Y., Yasukawa, T., Ohta, S., Akira, S., Ishihara, K., Watanabe, K., Suzuki, T. (2004). Codon-specific translational defect caused by a wobble modification deficiency in mutant tRNA from a human mitochondrial disease. Proc. Natl. Acad. Sci. USA. 101(42), 15070-15075.

28. Hino, N., Suzuki, T., Yasukawa, T., Seio, K., Watanabe, K., Ueda, T. (2004). The pathogenic A4269G mutation in human mitochondrial tRNA(Ile) alters the T-stem structure and decreases the binding affinity for elongation factor Tu. Genes Cells 9(3), 243-252.

29. Bowmaker, M., Yang, M. Y., Yasukawa, T., Reyes, A., Jacobs, H. T., Huberman, J. A., Holt, I. J. (2003). Mammalian mitochondrial DNA replicates bidirectionally from an initiation zone. J. Biol. Chem. 278(51), 50961-50969.

30. Toompuu, M., Yasukawa, T., Suzuki, T., Hakkinen, T., Spelbrink, J. N., Watanabe, K., Jacobs, H. T. (2002). The 7472insC mitochondrial DNA mutation impairs the synthesis and extent of aminoacylation of tRNASer(UCN) but not its structure or rate of turnover. J. Biol. Chem. 277(25), 22240-22250.

31. Yasukawa, T., Suzuki, T., Ishii, N., Ohta, S., Watanabe, K. (2001). Wobble modification defect in tRNA disturbs codon-anticodon interaction in a mitochondrial disease. EMBO J. 20(17), 4794-4802.

32. Yasukawa, T., Hino, N., Suzuki, T., Watanabe, K., Ueda, T., Ohta, S. (2000). A pathogenic point mutation reduces stability of mitochondrial mutant tRNA(Ile). Nucleic Acids Res. 28(19), 3779-3784.

33. Yasukawa, T., Suzuki, T., Ishii, N., Ueda, T., Ohta, S., Watanabe, K. (2000). Defect in modification at the anticodon wobble nucleotide of mitochondrial tRNA(Lys) with the MERRF encephalomyopathy pathogenic mutation. FEBS Lett. 467(2-3), 175-178.

34. Yasukawa, T., Suzuki, T., Suzuki, T., Ueda, T., Ohta, S., Watanabe, K. (2000). Modification defect at anticodon wobble nucleotide of mitochondrial tRNAs(Leu)(UUR) with pathogenic mutations of mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes. J. Biol. Chem. 275(6), 4251-4257.

【英語総説・著書など】

35. Yasukawa, T.*, Kang D. (2023). Assessing TFAM binding to human mitochondrial DNA. In Methods in Molecular Biology (Vol. 2615 pp.139-151). 【*corresponding author】

36. Yasukawa, T.*, Matsuda, S., Kang D. (2020). Epigenetic features of mitochondrial DNA (Chapter 3, pp. 71-85). In The Human Mitochondrial Genome. Gasparre, G., Porcelli, A. M. (eds.). Academic Press. (Cambridge, USA). 【*corresponding author】

37. Yasukawa, T.*, Kang D. (2018). An overview of mammalian mitochondrial DNA replication mechanisms. J. Biochem. 164(3), 183-193 【*corresponding author】

38. Yasukawa, T., Poulton, J. (2009). Mitochondrial DNA Replication. In Molecular Themes in DNA Replication. Cox, L. S. (ed.). (pp.316-345). London: Royal Society of Chemistry.

39. Reyes, A., Yasukawa, T., Cluett, T. J., Holt, I. J. (2009). Analysis of mitochondrial DNA by two-dimensional agarose gel electrophoresis. In Methods in Molecular Biology (Vol. 554 pp.15-35).

40. Reyes, A., Yasukawa, T., Holt, I. J. (2007). Analysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis. In Methods in Molecular Biology (Vol. 372 pp.219-232).

41. Kirino, Y., Yasukawa, T., Suzuki, T., Suzuki, T., Watanabe, K. (2003). Molecular mechanism of MELAS caused by taurine-modification defect in mitochondrial tRNA. In Korczyn, A. D. (Ed.) from Third International Congress on Vascular Dementia (pp.69-73).

42. Yasukawa, T., Suzuki, T., Ohta, S., Watanabe, K. (2002). Wobble modification defect suppresses translational activity of tRNAs with MERRF and MELAS mutations. Mitochondrion 2(1-2), 129-141.

43. Yasukawa, T., Suzuki, T., Ueda, T., Hayashi, J., Ohta, S., Watanabe, K. (1998). Stability of mitochondrial tRNA molecules with pathogenic point mutations. Nucleic Acids Symposium Series (Vol. 39 pp.257-258).

【日本語総説・著書など】

44. 安川武宏、康東天（2022）　「ミトコンドリアDNAの複製・維持機構と関連疾患」　医学のあゆみ　281(12), 1121-1127. 医歯薬出版

45. 安川武宏（2021）「ミトコンドリアゲノムの複製・維持」　ミトコンドリアダイナミクス　第４章第３節　pp121-129. ISBN 978-4-86043-746-6 C3045（株）エヌ・ティー・エス（東京）

46. 安川武宏、康東天（2019）　「ミトコンドリアゲノムの複製・維持機構」　実験医学　37(12), 24-30. 羊土社

47. 安川武宏、康東天（2018）　「ミトコンドリアDNAの維持システム Up-to-Date」メディカル・サイエンス・ダイジェスト　44(10), 17-20.　ニューサイエンス社

48. 安川武宏（2017）　「ミトコンドリアDNAのユニークな複製メカニズム」　医学のあゆみ　260(1), 5-10.　医歯薬出版

49. 太田成男、安川武宏（2012）「ミトコンドリアDNAの構造と発現制御」ミトコンドリア病－ up to date　月刊　臨床神経科学　30(9), 988-991. 中外医学社

50. 安川武宏（2010）「海外研究生活のすすめ」　せるてく・あらかると　細胞工学　vol. 29, No. 5, 474-476.　学研メディカル秀潤社

51. 安川武宏（2009）「国際色豊かな環境で」　ラボレポート―独立編　実験医学　vol. 27, No. 18, 3040-3042. 羊土社

52. 安川武宏（2005）「ミトコンドリアDNAのユニークな複製機構と転写機構」　タンパク質核酸酵素　50(14), 1727-1731.　共立出版

53. 安川武宏（2005）「ミトコンドリアDNAの複製メカニズム」　細胞工学　vol. 24, No. 8, 809-813. 秀潤社

54. 安川武宏（2004）「ミトコンドリアDNA複製機構のパラダイムシフト」　Molecular Medicine 41(3), 269-276. 中山書店

55. 安川武宏（2002）「ミトコンドリアDNAの複製機序と修復機構」　ミトコンドリアとミトコンドリア病　日本臨牀増刊号 60 (増刊号4), 24-29. 日本臨牀社

56. 安川武宏、鈴木勉、太田成男、渡辺公綱（2002）「変異ミトコンドリアtRNAの修飾欠損とdecoding異常」　ミトコンドリアとミトコンドリア病　日本臨牀増刊号　60 (増刊号4), 197-201.　日本臨牀社

57. 安川武宏、太田成男（2001）「サイブリッドの作製法と大量培養法」in 新ミトコンドリア学. 内海・井上　監修、共立出版（東京）．pp.392-394.

58. 太田成男、安川武宏、上田卓也（2000）「ミトコンドリア病とtRNA」in RNA研究の最前線.志村・渡辺　共編、シュプリンガ－・フェアラーク東京（東京）．pp.188-200.　

59. 安川武宏、渡辺公綱、太田成男（2000）「ミトコンドリア病発症研究の新しいアプローチ―ミトコンドリアtRNAアンチコドンの異常」　化学と生物　38 (11), 714-721.学会出版センター

60. 太田成男、安川武宏（2000）「ミトコンドリア脳筋症―ミトコンドリア変異tRNAのwobbling異常」　細胞工学　19, 596-604.　秀潤社

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安川 武宏（ヤスカワ タケヒロ）

安川武宏（ヤスカワタケヒロ）