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中西友子（ナカニシトモコ）

研究テーマ	アデノベクターによるゲノム編集システム構築、遺伝子改変マウスによる発生メカニズムの解析
研究業績（論文）	1. Kise S, Iijima A, Nagao C, Okada T, Mano H, Nishikawa M, Ikushiro S, Kanemoto Y, Kato S, Nakanishi T, Sato S, Yasuda K, Sakaki T. Functional analysis of vitamin D receptor (VDR) using adenovirus vector. J Steroid Biochem Mol Biol, 2023, 230:106275. 2. Nagao C, Kise S, Iijima A, Okada T, Nakanishi T, Sato S, Yasuda K, Sakaki T. Expression of rat Cyp27b1 in HepG2 cells using adenovirus vector and its application to evaluation of self-made and commercially available anti-Cyp27b1 antibodies. J Nutr Sci Vitaminol (Tokyo). 2023, in press 3. Kato Y, Tabata H, Sato K, Nakamura M, Saito I and ＊Nakanishi T. Adenovirus Vectors Expressing Eight Multiplex Guide RNAs of CRISPR/Cas9 Efficiently Disrupted Diverse Hepatitis B Virus Gene Derived from Heterogeneous Patient. Int J Mol Sci. 2021, 22(19):10570. 4. Nakanishi T(Corresponding author), Maekawa A, Suzuki M, Tabata H, Sato K, Mori M and Saito I. Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing. Sci Rep. 2021, 11(1): 3961 5. Nakanishi T, Maekawa A, Tabata H, Yoshioka T, Pei Z, Sato K, Mori M, Kato M and Saito I. Highly multiplex guide RNA expression units of CRISPR/Cas9 were completely stable using cosmid amplification in a novel polygonal structure. J Gene Med. 2019, 21(11): e3115 6. Bilotta FL, Arcidiacono B, Messineo S, Greco M., Chiefari E, Britti D, Nakanishi T, Foti DP, Brunetti A: Insulin and osteocalcin: further evidence for a mutual cross-talk. Endocrine 2018, 59(3): 622-632 7. Oji A, Amano T, Maeta Y, Hori N, Hatsuzawa K, Sato K and *Nakanishi T. Fate of methylated/unmethylated H19 imprinting control region after paternal and maternal pronuclear injection. Exp Animals. 2017, 66(4):367-378 8. Pazienza V, Panebianco C, Rapp F, Memoli D, Borghesan M, Cannito S, Oji A, Mazza G, Tamburrino D, Fusai G, Barone R, Bolasco G, Villarroya F, Villarroya J, Hatsuzawa K, Cappello F, Tarallo R, Nakanishi T, Vinciguerra M: Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesis. Epigenetics Chromatin. 2016, 9: 45 9. Pazienza V, Borghesan M, Mazza T, Sheedfar F, Panebianco C, Williams R, Mazzoccoli G, Andriulli A, Nakanishi T, Vinciguerra M. SIRT1-metabolite binding histone macroH2A1.1 protects hepatocytes against lipid accumulation. Aging. 2014, 6: 35-47 10. Nakanishi T, Saito R, Taniguchi M, Oda H, Soma A, Yasunaga M, Yamane M, Sato K. In vivo determination of vitamin D function using transgenic mice carrying a human osteocalcin luciferase reporter gene. BioMed Res Int. 2013, 2013: 895706 11. Yasunaga M, Masui E, Oji A, Soma A, Osaki M, Nakanishi T, Sato K. Identification of the control region of pancreatic expression of Bmp4 in vitro and in vivo. PLoS One. 3013, 8: e61821 12. Soma A, Sato K, Nakanishi T (corresponding author). Visualization of inactive X chromosome in preimplantation embryos utilizing MacroH2A–EGFP transgenic mouse. Genesis. 2013, 51: 259-267 13. Nakanishi T, Kokubun K, Oda H, Aoki M, Soma A, Taniguchi M, Kazuki Y, Oshimura M, Sato K. Bioluminescence imaging of bone formation using hairless osteocalcin-luciferase transgenic mice. Bone. 2012, 51: 369-375 14. Oumi N, Amano-Taniguchi K, Miyashita-Kanai A, Yasunaga M, Nakanishi T, Sato K. A crucial role of bone morphogenetic protein signaling in the wound healing response in acute liver injury induced by carbon tetrachloride. Int J Hepatol. 2012, 2012: 476820 15. Yasunaga M, Oumi N, Osak, M, Kazuki Y, Nakanishi T, Oshimura M, Sato K. Establishment and characterization of a transgenic mouse model for in vivo imaging of Bmp4 expression in the pancreas. PLoS One. 2011, 6: e24956 16. Takahashi Y, Tsuji S, Kazuki Y, Noguchi M, Arifuku I, Umebayashi Y, Nakanishi T, Oshimura M, Sato K. Development of evaluation system for bioactive substances using human artificial chromosome-mediated osteocalcin gene expression. J Biochem. 2010, 148: 29-34 17. Kimura M, Kim E, Kang W, Yamashita M, Saigo M, Yamazaki T, Nakanishi T, Kashiwabara SI, Baba T. Functional roles of sperm hyaluronidases, HYAL5 and SPAM1, in fertilization in mice. Biol Reprod. 2009, 81: 939-947 18. Hirata M, Amano K, Miyashita A, Yasunaga M, Nakanishi T, Sato K. Establishment and characterization of hepatic stem-like cell lines from normal adult rat liver. J Biochem. 2009, 145: 51-58 19. Nakanishi T (corresponding author), Ishibashi N, Kubota H, Inoue K, Ogonuki N, Ogura A, Kashiwabara S, Baba T. Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains. J Reprod Dev. 2008, 54: 244-249 20. Kashiwabara　S, Nakanishi T, Kimura M, Baba T. Non-canonical poly(A) polymerase in mammalian gametogenesis. Biochim Biophys Acta. 2008, 1779: 230-238 21. Nakanishi T, Kumagai S, Kimura M, Watanabe H, Sakurai T, Kimura M, Kashiwabara S, Baba T. Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells. Biochem Biophys Res Commun. 2007, 364: 14-19

研究テーマ

アデノベクターによるゲノム編集システム構築、遺伝子改変マウスによる発生メカニズムの解析

研究業績（論文）

1. Kise S, Iijima A, Nagao C, Okada T, Mano H, Nishikawa M, Ikushiro S, Kanemoto Y, Kato S, Nakanishi T, Sato S, Yasuda K, Sakaki T. Functional analysis of vitamin D receptor (VDR) using adenovirus vector. J Steroid Biochem Mol Biol, 2023, 230:106275.
2. Nagao C, Kise S, Iijima A, Okada T, Nakanishi T, Sato S, Yasuda K, Sakaki T. Expression of rat Cyp27b1 in HepG2 cells using adenovirus vector and its application to evaluation of self-made and commercially available anti-Cyp27b1 antibodies. J Nutr Sci Vitaminol (Tokyo). 2023, in press
3. Kato Y, Tabata H, Sato K, Nakamura M, Saito I and ＊Nakanishi T. Adenovirus Vectors Expressing Eight Multiplex Guide RNAs of CRISPR/Cas9 Efficiently Disrupted Diverse Hepatitis B Virus Gene Derived from Heterogeneous Patient. Int J Mol Sci. 2021, 22(19):10570.
4. Nakanishi T(Corresponding author), Maekawa A, Suzuki M, Tabata H, Sato K, Mori M and Saito I. Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing. Sci Rep. 2021, 11(1): 3961
5. Nakanishi T, Maekawa A, Tabata H, Yoshioka T, Pei Z, Sato K, Mori M, Kato M and Saito I. Highly multiplex guide RNA expression units of CRISPR/Cas9 were completely stable using cosmid amplification in a novel polygonal structure. J Gene Med. 2019, 21(11): e3115
6. Bilotta FL, Arcidiacono B, Messineo S, Greco M., Chiefari E, Britti D, Nakanishi T, Foti DP, Brunetti A: Insulin and osteocalcin: further evidence for a mutual cross-talk. Endocrine 2018, 59(3): 622-632
7. Oji A, Amano T, Maeta Y, Hori N, Hatsuzawa K, Sato K and *Nakanishi T. Fate of methylated/unmethylated H19 imprinting control region after paternal and maternal pronuclear injection. Exp Animals. 2017, 66(4):367-378
8. Pazienza V, Panebianco C, Rapp F, Memoli D, Borghesan M, Cannito S, Oji A, Mazza G, Tamburrino D, Fusai G, Barone R, Bolasco G, Villarroya F, Villarroya J, Hatsuzawa K, Cappello F, Tarallo R, Nakanishi T, Vinciguerra M: Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesis. Epigenetics Chromatin. 2016, 9: 45
9. Pazienza V, Borghesan M, Mazza T, Sheedfar F, Panebianco C, Williams R, Mazzoccoli G, Andriulli A, Nakanishi T, Vinciguerra M. SIRT1-metabolite binding histone macroH2A1.1 protects hepatocytes against lipid accumulation. Aging. 2014, 6: 35-47
10. Nakanishi T, Saito R, Taniguchi M, Oda H, Soma A, Yasunaga M, Yamane M, Sato K. In vivo determination of vitamin D function using transgenic mice carrying a human osteocalcin luciferase reporter gene. BioMed Res Int. 2013, 2013: 895706
11. Yasunaga M, Masui E, Oji A, Soma A, Osaki M, Nakanishi T, Sato K. Identification of the control region of pancreatic expression of Bmp4 in vitro and in vivo. PLoS One. 3013, 8: e61821
12. Soma A, Sato K, Nakanishi T (corresponding author). Visualization of inactive X chromosome in preimplantation embryos utilizing MacroH2A–EGFP transgenic mouse. Genesis. 2013, 51: 259-267
13. Nakanishi T, Kokubun K, Oda H, Aoki M, Soma A, Taniguchi M, Kazuki Y, Oshimura M, Sato K. Bioluminescence imaging of bone formation using hairless osteocalcin-luciferase transgenic mice. Bone. 2012, 51: 369-375
14. Oumi N, Amano-Taniguchi K, Miyashita-Kanai A, Yasunaga M, Nakanishi T, Sato K. A crucial role of bone morphogenetic protein signaling in the wound healing response in acute liver injury induced by carbon tetrachloride. Int J Hepatol. 2012, 2012: 476820
15. Yasunaga M, Oumi N, Osak, M, Kazuki Y, Nakanishi T, Oshimura M, Sato K. Establishment and characterization of a transgenic mouse model for in vivo imaging of Bmp4 expression in the pancreas. PLoS One. 2011, 6: e24956
16. Takahashi Y, Tsuji S, Kazuki Y, Noguchi M, Arifuku I, Umebayashi Y, Nakanishi T, Oshimura M, Sato K. Development of evaluation system for bioactive substances using human artificial chromosome-mediated osteocalcin gene expression. J Biochem. 2010, 148: 29-34
17. Kimura M, Kim E, Kang W, Yamashita M, Saigo M, Yamazaki T, Nakanishi T, Kashiwabara SI, Baba T. Functional roles of sperm hyaluronidases, HYAL5 and SPAM1, in fertilization in mice. Biol Reprod. 2009, 81: 939-947
18. Hirata M, Amano K, Miyashita A, Yasunaga M, Nakanishi T, Sato K. Establishment and characterization of hepatic stem-like cell lines from normal adult rat liver. J Biochem. 2009, 145: 51-58
19. Nakanishi T (corresponding author), Ishibashi N, Kubota H, Inoue K, Ogonuki N, Ogura A, Kashiwabara S, Baba T. Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains. J Reprod Dev. 2008, 54: 244-249
20. Kashiwabara　S, Nakanishi T, Kimura M, Baba T. Non-canonical poly(A) polymerase in mammalian gametogenesis. Biochim Biophys Acta. 2008, 1779: 230-238
21. Nakanishi T, Kumagai S, Kimura M, Watanabe H, Sakurai T, Kimura M, Kashiwabara S, Baba T. Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells. Biochem Biophys Res Commun. 2007, 364: 14-19

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研究業績詳細

中西 友子（ナカニシ トモコ）

中西友子（ナカニシトモコ）