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研究業績詳細

谷田以誠（タニダイセイ）

研究テーマ	神経細胞におけるオートファジー分子基盤の研究
研究業績（論文）	2023年「英文原著・総説」 1. Tanida I, Yamaguchi J, Kakuta S, Uchiyama Y: Osmium-Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells. Methods Mol Biol. 2023;2564:287-297. doi: 10.1007/978-1-0716-2667-2_15. 2. Tanida I, Yamaguchi J, Suzuki C, Kakuta S, Uchiyama Y: Recent advances in in-resin correlative light and electron microscopy of Epon-embedded cells. Microscopy (Oxf). 2023 May 22:dfad028. doi: 10.1093/jmicro/dfad028. 3. Aoyama S, Nishida Y, Uzawa H, Himuro M, Kanai A, Ueki K, Ito M, Iida H, Tanida I, Miyatsuka T, Fujitani Y, Matsumoto M, Watada H: Monitoring autophagic flux in vivo revealed its physiological response and significance of heterogeneity in pancreatic beta cells. Cell Chem Biol. 2023 Mar 16:S2451-9456(23)00060-0. doi:10.1016/j.chembiol.2023.03.001. PMID: 36944338 2022年「英文原著」 1. Sou YS, Yamaguchi J, Kameda H, Masuda K, Maeda Y, Uchiyama Y, Koike M: GPHR-mediated acidification of the Golgi lumen is essential for cholesterol biosynthesis in the brain. FEBS Lett. 2022, 596:2873-2888. 2. Suzuki C, Yamaguchi J, Sanada T, Oliva Trejo JA, Kakuta S, Shibata M, Tanida I, Uchiyama Y: Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins. Sci Rep. 2022, 12:11662. （プレスリリースhttps://www.juntendo.ac.jp/news/00562.html） 3. Sanada T, Yamaguchi J, Furuta Y, Kakuta S, Tanida I, Uchiyama Y: In-resin CLEM of Epon-embedded cells using proximity labeling. Sci Rep. 2022, 12:11130. （プレスリリースhttps://www.juntendo.ac.jp/news/00566.html） 4. Suzuki A, Iwaya C, Ogata K, Yoshioka H, Shim J, Tanida I, Komatsu M, Tada N, Iwata J: Impaired GATE16-mediated exocytosis in exocrine tissues causes Sjögren’s syndrome-like exocrinopathy. Cell Mol Life Sci. 2022, 79:307. 「和文原著」 1. 谷田以誠, 山口隼司, 角田宗一郎, 内山安男、エポン樹脂包埋試料中の蛍光タンパク質を観察する～In-resin CLEMへのアプローチ、解剖学雑誌 2022, 97:13-16. 2021年以前「英文原著」 1. Tanida I, Haruta T, Suga M, Takei S, Takebe A, Furuta Y, Yamaguchi J, Oliva Trejo JA, Kakuta S, Uchiyama Y. Membranous Structures Directly Come in Contact With p62/SQSTM1 Bodies. J Histochem Cytochem. 2021 Apr 22:221554211011423. doi: 10.1369/00221554211011423. PMID: 33884901 2. Klionsky DJ, Tanida I, Uchiyama Y, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1. Autophagy. 2021 Jan;17(1):1-382. doi: 10.1080/15548627.2020.1797280. Epub 2021 Feb 8. PMID: 33634751 3. Tanida I, Furuta Y, Yamaguchi J, Kakuta S, Oliva Trejo JA, Uchiyama Y. Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins. Sci Rep. 2020 Dec 14;10(1):21871. doi: 10.1038/s41598-020-78879-x. PMID: 33318540 （プレスリリースhttps://www.juntendo.ac.jp/news/20201223-06.html） 4. Ogawa M, Shirasago Y, Tanida I, Kakuta S, Uchiyama Y, Shimojima M, Hanada K, Saijo M, Fukasawa M. Structural basis of antiviral activity of caffeic acid against severe fever with thrombocytopenia syndrome virus. J Infect Chemother. 2021 Feb;27(2):397-400. doi: 10.1016/j.jiac.2020.10.015. Epub 2020 Oct 24. PMID: 3310943 5. Tanida I, Kakuta S, Oliva Trejo JA, Uchiyama Y. Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein. Sci Rep. 2020 Jul 9;10(1):11314. doi: 10.1038/s41598-020-68191-z. PMID: 32647231 6. Oliva Trejo JA, Tanida I, Suzuki C, Kakuta S, Tada N, Uchiyama Y. Characterization of starvation-induced autophagy in cerebellar Purkinje cells of pHluorin-mKate2-human LC3B transgenic mice. Sci Rep. 2020 Jun 15;10(1):9643. doi: 10.1038/s41598-020-66370-6. PMID: 32541814; PMCID: PMC7295967. 7. Morimoto K, Suzuki N, Tanida I, Kakuta S, Furuta Y, Uchiyama Y, Hanada K, Suzuki Y, Yamaji T: Blood group P1 antigen-bearing glycoproteins are functional but less efficient receptors of Shiga toxin than conventional glycolipid-based receptors. J Biol Chem. 2020, jbc.RA120.013926. doi: 10.1074/jbc.RA120.013926. 8. Ogawa M, Takada N, Shizukuishi S, Tomokiyo M, Chang B, Yoshida M, Kakuta S, Tanida I, Ryo A, Guan JL, Takeyama H, Ohnishi M: Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization. Commun Biol. 2020, 3:25. doi: 10.1038/s42003-020-0753-3. 3 9. Suzuki C, Tanida I, Oliva Trejo JA, Kakuta S, Uchiyama Y: Autophagy Deficiency in Renal Proximal Tubular Cells Leads to an Increase in Cellular Injury and Apoptosis under Normal Fed Conditions. Int J Mol Sci. 2019, 21:155. doi: 10.3390/ijms21010155. 10. Okemoto-Nakamura Y, Tanida I, Yamaji T, Hanada K, and Hagiwara K: A PRNP-disrupted human neuroblastoma cell line and its stable transformants expressing prion protein variants. BPB reports, 2019, 52:357-65. doi: 10.1111/j.1348-0421.2008.00046.x. 11. Yazawa R, Nishida Y, Aoyama S, Tanida I, Miyatsuka T, Suzuki L, Himuro M, Haruna H, Takubo N, Shimizu T, Watada H: Establishment of a system for screening autophagic flux regulators using a modified fluorescent reporter and CRISPR/Cas9. Biochem Biophys Res Commun. 516:686-692, 2019 12. Sou YS, Kakuta S, Kamikubo Y, Niisato K, Sakurai T, Parajuli LK, Tanida I, Saito H, Suzuki N, Sakimura K, Maeda Y, Kinoshita T, Uchiyama Y, Koike M: Cerebellar Neurodegeneration and Neuronal Circuit Remodeling in Golgi pH Regulator-Deficient Mice. eNeuro. 6. pii: ENEURO.0427-18.2019 13. Shirasago Y, Inamori Y, Suzuki T, Tanida I, Suzuki T, Sugiyama K, Wakita T, Hanada K, Fukasawa M: Inhibition Mechanisms of Hepatitis C Virus Infection by Caffeic Acid and Tannic Acid. Biol Pharm Bull. 42:770-777, 2019 14. Suzuki C, Tanida I, Ohmuraya M, Oliva Trejo JA, Kakuta S, Sunabori T, Uchiyama Y. Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury. Int J Mol Sci. 5;20. pii: E1711, 2019 15. Nitta A, Hori K, Tanida I, Igarashi A, Deyama Y, Ueno T, Kominami E, Sugai M, Aoki K: Blocking LC3 lipidation and ATG12 conjugation reactions by ATG7 mutant protein containing C572S. Biochem Biophys Res Commun. 508:521-526, 2018 16. Ogawa M, Matsuda R, Takada N, Tomokiyo M, Yamamoto S, Shizukusihi S, Yamaji T, Yoshikawa Y, Yoshida M, Tanida I, Koike M, Murai M, Morita H, Takeyama H, Ryo A, Guan JL, Yamamoto M, Inoue JI, Yanagawa T, Fukuda M, Kawabe H, Ohnishi M: Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1-mediated K63-linked ubiquitination. Cell Microbiol. 20:e12846, 2018 17. Tanida-Miyake E, Koike M, Uchiyama Y, Tanida I: Optimization of mNeonGreen for Homo sapiens increases its fluorescent intensity in mammalian cells. PLoS ONE. 13 :e0191108, 2018 18. Yamaguchi J, Suzuki C, Nanao T, Kakuta S, Ozawa K, Tanida I, Saitoh T, Sunabori T, Komatsu M, Tanaka K, Aoki S, Sakimura K, Uchiyama Y: Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis. Autophagy. 14:764-777, 2018 19. Tanida I, Ueno T, Uchiyama Y: Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses. Methods Enzymol. 587:87-96, 2017 20. Shirasago Y, Shimizu Y, Tanida I, Suzuki T, Suzuki R, Sugiyama K, Wakita T, Hanada K, Yagi K, Kondoh M, Fukasawa M: Occludin-Knockout Human Hepatic Huh7.5.1-8-Derived Cells Are Completely Resistant to Hepatitis C Virus Infection. Biol Pharm Bull. 39:839-48, 2016 21. Klionsky DJ, Tanida I, et al. (2467人中2071番): Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12:1-222, 2016 22. Rinchai D, Riyapa D, Buddhisa S, Utispan K, Titball RW, Stevens MP, Stevens JM, Ogawa M, Tanida I, Koike M, Uchiyama Y, Ato M, Lertmemongkolchai G: Macroautophagy is essential for killing of intracellular Burkholderia pseudomallei in human neutrophils. Autophagy. 11: 784-755, 2015 23. Tanida I, Shirasago Y, Suzuki R, Abe R, Wakita T, Hanada K, Fukasawa M: Caffeic acid, a coffee-related organic acid, inhibits the propagation of hepatitis C virus. Jpn J Infect Dis. 68: 268-275, 2014 24. Tanida I, Ueno T, Uchiyama Y: A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian. Autophagy PLoS ONE. 9: e110600, 2014 25. Harada-Hada K, Harada K, Kato F, Hisatsune J, Tanida I, Ogawa M, Asano S, Sugai M, Hirata M, Kanematsu T.: Phospholipase C-related catalytically inactive protein participates in the autophagic elimination of Staphylococcus aureus infecting mouse embryonic fibroblasts. PLoS ONE. 9:e98285, 2014 26. Asano S, Nemoto T, Kitayama T, Harada K, Zhang J, Harada K, Tanida I, Hirata M, Kanematsu T: Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion. Biol Open 3:463-74, 2014 27. Tanida I, Koike M, Nanao T, Tada N, Iwata JI, Ueno T, Kominami E, Uchiyama Y: Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons. PLoS one 8: e63568, 2013 (*: equally contributed) 28. Umebayashi H, Mizokami A, Matsuda M, Harada K, Takeuchi H, Tanida I, Hirata M, Kanematsu T: Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation. Biochem Biophys Res Commun. 432: 268-74, 2013 29. Tanida I, Yamasaki M, Komatsu M, Ueno T: The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation. Autophagy 8:88-97, 2012 30. Klionsky DJ, Tanida I, et al. (1204人中1082番): Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8: 445-544, 2012 31. Takagi-Akiba M, Asanuma K, Tanida I, Tada N, Oliva Trejo JA, Nonaka K, Asanuma E, Kominami E, Ueno T, Tomino Y: Doxorubicin-induced glomerulosclerosis with proteinuria in GFP-GABARAP transgenic mice. Am J Physiol Renal Physiol 302:F380-9, 2012 32. Inami Y, Yamashina S, Izumi K, Ueno T, Tanida I, Ikejima K, Watanabe S: Hepatic steatosis inhibits autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression. Biochem Biophys Res Commun 412: 618-25, 2011 33. Ezaki J, Matsumoto N, Takeda-Ezaki M, Komatsu M, Takahashi K, Hiraoka Y, Taka H, Fujimura T, Takehana K, Yoshida M, Iwata J, Tanida I, Furuya N, Zheng DM, Tada N, Tanaka K, Kominami E, Ueno T: Liver autophagy contributes to the maintenance of blood glucose and amino acid levels. Autophagy. 7: 727-736, 2011 34. Saiki S, Sasazawa Y, Imamichi Y, Kawajiri S, Fujimaki T, Tanida I, Kobayashi H, Sato F, Sato S, Ishikawa K, Imoto M, Hattori N: Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7: 176-87, 2011 35. Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T: Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol 183: 7278-7285, 2009 36. Tanida I, Fukasawa M, Ueno T, Kominami E, Wakita T, Hanada K: Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles. Autophagy 5: 937-945, 2009 37. Mizui T, Yamashina S, Tanida I, Takei Y, Ueno T, Sakamoto N, Ikejima K, Kitamura T, Enomoto N, Sakai T, Kominami E, Watanabe S: Inhibition of hepatitis C virus replication by chloroquine targeting virus-associated autophagy. J Gastroenterol 45: 195-203, 2009 38. Takahashi K, Ueno T, Tanida I, Minematsu-Ikeguchi N, Murata M, Kominami E.; Characterization of CAA0225, a novel inhibitor specific for cathepsin L, as a probe for autophagic proteolysis. Biol Pharm Bull 32: 475-9, 2009 39. Haruna K, Suga Y, Muramatsu S, Taneda K, Mizuno Y, Ikeda S, Ueno T, Kominami E, Tanida I, Hanada K: Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes. J Dermatol Sci 52: 213-215, 2008 40. Okemoto-Nakamura Y, Yamakawa Y, Hanada K, Tanaka K, Miura M, Tanida I, Nishijima M, Hagiwara K: A Synthetic Fibril Peptide Promotes Clearance of Scrapie Prion Protein by Lysosomal Degradation. Microbiol Immunol 52: 357-365, 2008 41. Ueno T, Sato W, Horie Y, Komatsu M, Tanida I, Yoshida M, Ohshima S, Mak TW, Watanabe S, Kominami E: Loss of Pten, a tumor suppressor, causes the strong inhibition of autophagy without affecting LC3 lipidation. Autophagy 4: 692-700, 2008 42. Klionsky DJ, Tanida I, et al. (236人中202番): Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4: 151-175, 2008 43. Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K: Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Autophagy 4: 131-134. 2008 44. Uchio N, Oma Y, Toriumi K, Sasagawa N, Tanida I, Fujita E, Kouroku Y, Kuroda R, Momoi T, Ishiura S: Endoplasmic reticulum stress caused by aggregate-prone proteins containing homopolymeric amino acids. FEBS J 274: 5619-27, 2007 45. Mizokami A, Kanematsu T, Ishibashi H, Yamaguchi T, Tanida I, Takenaka K, Nakayama KI, Fukami K, Takenawa T, Kominami E, Moss SJ, Yamamoto T, Nabekura J, Hirata M: Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABAA receptors to the cell surface. J Neurosci 27: 1692-1701, 2007 46. Kouroku Y, Fujita E, Tanida I, Ueno T, Isoai A, Kumagai H, Ogawa S, Kaufman RJ, Kominami E, Momoi T: ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation. Cell Death Differ 14: 230-239, 2007 47. Demarchi F, Bertoli C, Copetti T, Tanida I, Brancolini C, Eskelinen EL, Schneider C.; Calpain is required for macroautophagy in mammalian cells. J Cell Biol. 175: 595-605, 2006 48. Tanida I, Wakabayashi M, Kanematsu T, Minematsu-Ikeguchi N, Sou YS, Hirata M, Ueno T, Kominami E: Lysosomal turnover of GABARAP-phospholipid conjugate is activated during differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. Autophagy 2: 264-271, 2006 49. Tanida I, Sou YS, Minematsu-Ikeguchi N, Ueno T, Kominami E: Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3. FEBS J 273: 2553-2562, 2006 50. Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, Ueno T, Koike M, Uchiyama Y, Kominami, E, Tanaka K: Loss of autophagy in the central nervous system causes neurodegeneration. Nature 441: 880-884, 2006 51. Sou Y, Tanida I, Komatsu M, Ueno T, Kominami E: Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP and GATE-16. J Biol Chem 281: 3017-3024, 2006 52. Iwata J, Ezaki J, Komatsu M, Yokota S, Ueno T, Tanida I, Chiba T, Tanaka K, Kominami E: Excess peroxisomes are degraded by autophagic machinery in mammals. J Biol Chem 281: 4035-4041, 2006 53. Kumanomidou T, Mizushima T, Komatsu M, Suzuki A, Tanida I, Sou Y, Ueno T, Kominami E, Tanaka K, Yamane T.; The Crystal Structure of Human Atg4b, a Processing and De-conjugating Enzyme for Autophagosome-forming Modifiers. J Mol Biol 355: 612-618, 2005 54. Koike M, Shibata M, Waguri S, Tanida I, Kominami E, Gotow T, Peters C, von Figura K, Mizushima N, Saftig P, Uchiyama Y: Participation of autophagy in storage of lysosomes in neurons from mouse model of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol 167: 1713-1728, 2005 55. Tanida I, Minematsu-Ikeguchi N, Ueno T, Kominami E: Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1: 84-91, 2005 56. Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T: Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 169: 425-434, 2005 57. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kanematsu T, Mori Y, Shinoda H, Hirata M, Kominami E, Kawano K.: Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains. J Biol Chem 280: 24610-24617, 2005 58. Tanida I, Ueno T, Kominami E: Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem 279: 47704-47710, 2004 59. Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K: A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J 23: 1977-1986, 2004 60. Tanida I, Sou YS, Ezaki J, Minematsu-Ikeguchi N, Ueno T, Kominami E: HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates. J Biol Chem 279: 36268-36276, 2004 61. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kominami E, Kawano K: 1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3. J Biomol NMR 29: 415-416, 2004 62. Jäger S, Bucci C, Tanida I, Ueno T, Kominami E, Saftig P, Eskelinen EL: Role for Rab7 in maturation of late autophagic vacuoles. J Cell Sci 117: 4837-4848, 2004 63. Asanuma K, Tanida I, Shirato I, Ueno T, Takahara H, Nishitani T, Kominami E, Tomino Y: MAP-LC3, a promising autophagosomal marker, is processed during the differentiation and recovery of podocytes from PAN nephrosis. FASEB J 17: 1165-1167, 2003 64. Nemoto T, Tanida I, Tanida-Miyake E, Minematsu-Ikeguchi N, Yokota M, Ohsumi M, Ueno T, Kominami E: The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification. J Biol Chem 278: 39517-39526, 2003 65. Yamazaki-Sato H, Tanida I, Ueno T, Kominami E: The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett 551: 71-77, 2003 66. Tanida I, Komatsu M, Ueno T, Kominami E: GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3. Biochem Biophys Res Commun 300: 637-644, 2003 67. Tanida I, Tanida-Miyake E, Komatsu M, Ueno T, Kominami E: Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J Biol Chem 277: 13739-13744, 2002 68. Tanida I, Nishitani T, Nemoto T, Ueno T, Kominami E: Mammalian Apg12p, but not the Apg12p-Apg5p conjugate, facilitates LC3 processing. Biochem Biophys Res Commun 296: 1164-1170, 2002 69. Tanida I, Tanida-Miyake E, Nishitani T, Komatsu M, Yamazaki H, Ueno T, Kominami E: Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs. Biochem Biophys Res Commun 292: 256-262, 2002 70. Komatsu M, Tanida I, Ueno T, Ohsumi M, Ohsumi Y, Kominami E: The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation. J Biol Chem 276: 9846-9854, 2001 71. Tanida I, Tanida-Miyake E, Ueno T, Kominami E: The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J Biol Chem 276: 1701-1706, 2001 72. Ichimura Y, Kirisako T, Takao T, Satomi Y, Shimonishi Y, Ishihara N, Mizushima N, Tanida I, Kominami E, Ohsumi M, Noda T, Ohsumi Y.: A ubiquitin-like system mediates protein lipidation. Nature 408: 488-492, 2000 73. Ezaki J, Tanida I, Kanehagi N, Kominami E: A lysosomal proteinase, the late infantile neuronal ceroid lipofuscinosis gene (CLN2) product, is essential for degradation of a hydrophobic protein, the subunit c of ATP synthase. J Neurochem: 72, 2573-2582, 1999 74. Ueno T, Ishidoh K, Mineki R, Tanida I, Murayama K, Kadowaki M, Kominami E: Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. J Biol Chem 274: 15222-15229, 1999 75. Tanida I, Mizushima N, Kiyooka M, Ohsumi M, Ueno T, Ohsumi Y, Kominami E: Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy. Mol Biol Cell 10: 1367-1379, 1999 76. Tanida I, Takita Y, Hasegawa A, Ohya Y, Anraku Y: Yeast Cls2p/Csg2p localized on the endoplasmic reticulum membrane regulates a non-exchangeable intracellular Ca2+ pool cooperatively with calcineurin. FEBS Lett 379: 38-42, 1996 77. Tanida I, Hasegawa A, Iida H, Ohya Y, Anraku Y: Cooperation of calcineurin and vacuolar H+-ATPase in intracellular Ca2+ homeostasis of yeast cells. J Biol Chem 270: 10113-10119, 1995 78. Yagi S, Yanagida M, Tanida I, Hasegawa A, Okumura K, Ra C: High-level expression of the truncated alpha chain of human high-affinity receptor for IgE as a soluble form by baculovirus-infected insect cells. Biochemical characterization of the recombinant product. Eur J Biochem 220: 593-598, 1994 79. Tanida I, Yanagida M, Maki N, Yagi S, Namiyama F, Kobayashi T, Hayano T, Takahashi N, Suzuki M: Yeast cyclophilin-related gene encodes a nonessential second peptidyl-prolyl cis-trans isomerase associated with the secretory pathway. Transplant Proc 23: 2856-2861, 1991 80. Ohya Y, Umemoto N, Tanida I, Ohta A, Iida H, Anraku Y: Calcium-sensitive cls mutants of Saccharomyces cerevisiae showing a Pet- phenotype are ascribable to defects of vacuolar membrane H+-ATPase activity. J Biol Chem 266: 13971-13977, 1991 81. Galons JP, Tanida I, Ohya Y, Anraku Y, Arata Y: A multinuclear magnetic resonance study of a cls11 mutant showing the Pet- phenotype of Saccharomyces cerevisiae. Eur J Biochem 193: 111-119, 1990 「英文総説」 1. Tanida I: Autophagosome formation and molecular mechanism of autophagy. Antioxid Redox Signal 14: 2201-14, 2011 2. Tanida I: Autophagy basics. Microbiol Immunol 55: 1-11, 2011 3. Tanida I, Ueno T, Kominami E: LC3 conjugation system in mammalian autophagy. Int J Biochem Cell Biol 36: 2503-2518, 2004 「和文総説」 1. 谷田以誠: C型肝炎とオートファジー、J. Gastrointest. Research 2015.23: 205-211 2. 谷田以誠：オートファジー-リソソーム分解系～疾患・生理機能との関わりと分子機構、内分泌・糖尿病・代謝内科 33: 319-325, 2011 3. 谷田以誠、上野隆、和栗聡：オートファジーの評価方法、実験医学 29: 2054-2062, 2011 4. 谷田以誠：哺乳類ATG結合系、蛋白質核酸酵素 10: 1490-1493, 2006 5. 谷田以誠：哺乳類オートファジーとATGコンジュゲーションシステム、医学のあゆみ 216: 505-508, 2006 6. 谷田以誠：Atg7/Apg7ヒトホモログ、生体の科学 55: 424-425, 2004 7. 谷田以誠：腎障害とLC3ユビキチン様修飾、実験医学 22: 311-318, 2003 8. 谷田以誠、木南英紀：オートファジーにおけるApg7タンパク質活性化酵素、生体の科学 54: 521-527, 2003 9. 谷田以誠：酵母のオートファジータンパク質Apg7pとヒトホモログ、生体の科学 52: 612,2001 10. 木南英紀、江崎淳二、谷田以誠：プロテオリピッドの分解とリソソーム、生体の科学 50: 140-144, 1999 「英文著書」 1. Tanida I, and Kioke M: TECHNIQUES FOR STUDYING AUTOPHAGY in Autophagy, Infection, and the Immune Response, Jackson W, & Swanson M ed., Wiley Blackwell, John Wiley and Sons, Inc., Hoboken, NJ, 19-32, 2014 2. Tanida I, Ueno T, Kominami E: In Vitro Assays of Lipidation of Mammalian Atg8 Homologs. Current Protocols in Cell Biology, 11.20. 1-11.20. 13, 2014 3. Tanida I, Waguri S: Measurement of autophagy in cells and tissues. Methods Mol Biol 648: 193-214, 2010 4. Tanida I, Ueno T, Kominami E: LC3 and autophagy. Methods Mol Biol 445: 77-88, 2008 5. Ueno T, Tanida I, Kominami E: Autophagy and neuromuscular diseases in Autophagy, Klionsky D, ed., Landes Bioscience, Georgetown, Texas, 264-286, 2004 「和文著書」 1. 淺沼克彦、谷田以誠: オートファジー評価法，腎臓病実験法ハンドブック，編者富野康日己，中外医学社，東京都新宿区，19-24, 2013 2. 谷田以誠: 遺伝子組み替え抗原とその免疫測定への応用，バイオ検査薬と機器・装置，編者山本重夫，シーエムシー，東京都千代田区，106-115，1996 「英文報告その他」　招待講演 1. Tanida I: Autophagy and Infection, Hua Hin 2013 Meeting, Hua Hin, Thailand, 2013 2. Tanida I: Autophagic Mechanism and Production of HCV Particles, World Congress of Virus and Infection-2010. Busan, Korea, 2010 3. Tanida I: Autophagy in naive hepatitis C virus production, 5th International Symposium on Autophagy. Shiga, Japan, 2009 4. Tanida I: Autophagy basics: International Symposium on Autophagy and Cell Death. Tokyo, Japan, 2009 5. Tanida I, Komatsu M, Iwata JI, Ezaki J, Ueno T, Kominami E: Mammalian Atg-conjugation systems: Their molecular and physiological functions. 19th Annual & International Meeting of the Japanese Association for Animal Cell Technology. Kyoto, Japan, 2006 6. Tanida I: The lysosomal turnover, but not a transient amount, of endogenous LC3 is a marker for autophagy. Gordon Research Conferences "Autophay in Stress, Development and Disease". Il Ciocco, Barga, Italy, 2005 7. Tanida I: Biochemistry of mammalian Apg genes. Gordon Research Conferences "Autophay in Stress, Development and Disease". Waterville, Maine, USA, 2003 8. Tanida I: Crosstalk between two ubiquitin-like modifications. III International Symposium on Autophagy. Osaka, 2002 インタビュー 1. Tanida I: SCIENCE WATCH INTERVIEW, THOMSON REUTERS・ SCIENCE WATCH・Special Topic of Autophagy http://archive.sciencewatch.com/ana/st/autophagy/09octSTAutoTani.pdf 「和文報告その他」シンポジウム企画・講演 1. 谷田以誠、甲賀大輔：シンポジウム「先端的電顕技術が切り拓くオルガネライメージングの世界」、第125回日本解剖学会総会全国学術集会、新潟2020 2. 谷田以誠、甲賀大輔：シンポジウム「細胞内スーパーイメージングの最先端」、第124回日本解剖学会総会全国学術集会、新潟2019 3. 谷田以誠、甲賀大輔：ワークショップ「細胞内スーパーイメージングの世界」、第124回日本分子生物学会、横浜2018 4. 谷田以誠、新崎恒平：「病原体感染におけるオルガネラ・ダイナミクス」シンポジウム、第87回日本生化学会大会、京都2014 　特許出願 1. 酵母プロテインジスルフィドイソメラ−ゼ　平成　２年　（特願平2-322620） 2. 昆虫細胞による可溶性ＩｇＥ結合受容体α鎖の製造方法　平成　５年　（特願平05-201991、公開番号:特開平07-031483、　平成１５年成立登録番号：3473973号） 3. 酵母により可溶性ＩｇＥ結合受容体α鎖を製造する方法　平成　４年　（特願平4-308896、公開番号：特開平06-153967） 4. ＩｇＥの測定方法　平成５年　（特願平05-218920、公開番号：特開平07-072150） 5. 免疫抑制物質のスクリーニング方法　平成　６年　（特願平06-116622、公開番号：特開平07-322891） 6. アレルギー反応誘発性ＩｇＥの特異的測定方法　平成　６年　（特願平06-217469、公開番号：特開平08-082592） 7. 免疫抑制物質のスクリーニング方法　平成　６年　（特願平06-116731、公開番号：特開平07-322982） 8. 免疫抑制物質のスクリーニング方法　平成　６年　（特願平06-116622、公開番号：特開平07-322891） 9. 免疫抑制剤高感受性変異株及びその使用　平成　７年　（特願平07-341819、公開番号：特開09-173053） 10. トランスジェニック非ヒト動物　平成１８年　（特願2006-284583、公開番号：特開2008-99601） 11. トランスジェニック非ヒト動物　平成１８年　（特願2006-284568、公開番号：特開2008-99600）　 12. ATG７変異体を用いたオートファジーの抑制方法　平成２４年　（特願2012-280082）　インタビュー 1. 内山　安男、上野　隆、谷田　以誠: ノーベル医学・生理学賞 “オートファジー”どう影響？、首都圏ネットワーク・NHK総合テレビ午後6時から

研究テーマ

神経細胞におけるオートファジー分子基盤の研究

研究業績（論文）

2023年
「英文原著・総説」
1. Tanida I, Yamaguchi J, Kakuta S, Uchiyama Y: Osmium-Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells. Methods Mol Biol. 2023;2564:287-297. doi: 10.1007/978-1-0716-2667-2_15.

2. Tanida I, Yamaguchi J, Suzuki C, Kakuta S, Uchiyama Y: Recent advances in in-resin correlative light and electron microscopy of Epon-embedded cells. Microscopy (Oxf). 2023 May 22:dfad028. doi: 10.1093/jmicro/dfad028.

3. Aoyama S, Nishida Y, Uzawa H, Himuro M, Kanai A, Ueki K, Ito M, Iida H, Tanida I, Miyatsuka T, Fujitani Y, Matsumoto M, Watada H: Monitoring autophagic flux in vivo revealed its physiological response and significance of heterogeneity in pancreatic beta cells. Cell Chem Biol. 2023 Mar 16:S2451-9456(23)00060-0. doi:10.1016/j.chembiol.2023.03.001. PMID: 36944338

2022年
「英文原著」
1. Sou YS, Yamaguchi J, Kameda H, Masuda K, Maeda Y, Uchiyama Y, Koike M: GPHR-mediated acidification of the Golgi lumen is essential for cholesterol biosynthesis in the brain. FEBS Lett. 2022, 596:2873-2888.

2. Suzuki C, Yamaguchi J, Sanada T, Oliva Trejo JA, Kakuta S, Shibata M, Tanida I, Uchiyama Y: Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins. Sci Rep. 2022, 12:11662.
（プレスリリースhttps://www.juntendo.ac.jp/news/00562.html）

3. Sanada T, Yamaguchi J, Furuta Y, Kakuta S, Tanida I, Uchiyama Y: In-resin CLEM of Epon-embedded cells using proximity labeling. Sci Rep. 2022, 12:11130.
（プレスリリースhttps://www.juntendo.ac.jp/news/00566.html）

4. Suzuki A, Iwaya C, Ogata K, Yoshioka H, Shim J, Tanida I, Komatsu M, Tada N, Iwata J: Impaired GATE16-mediated exocytosis in exocrine tissues causes Sjögren’s syndrome-like exocrinopathy. Cell Mol Life Sci. 2022, 79:307.

「和文原著」
1. 谷田以誠, 山口隼司, 角田宗一郎, 内山安男、エポン樹脂包埋試料中の蛍光タンパク質を観察する～In-resin CLEMへのアプローチ、解剖学雑誌 2022, 97:13-16.

2021年以前

「英文原著」
1. Tanida I, Haruta T, Suga M, Takei S, Takebe A, Furuta Y, Yamaguchi J, Oliva Trejo JA, Kakuta S, Uchiyama Y. Membranous Structures Directly Come in Contact With p62/SQSTM1 Bodies. J Histochem Cytochem. 2021 Apr 22:221554211011423. doi: 10.1369/00221554211011423. PMID: 33884901
2. Klionsky DJ, Tanida I, Uchiyama Y, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1. Autophagy. 2021 Jan;17(1):1-382. doi: 10.1080/15548627.2020.1797280. Epub 2021 Feb 8. PMID: 33634751
3. Tanida I, Furuta Y, Yamaguchi J, Kakuta S, Oliva Trejo JA, Uchiyama Y. Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins. Sci Rep. 2020 Dec 14;10(1):21871. doi: 10.1038/s41598-020-78879-x. PMID: 33318540 （プレスリリースhttps://www.juntendo.ac.jp/news/20201223-06.html）
4. Ogawa M, Shirasago Y, Tanida I, Kakuta S, Uchiyama Y, Shimojima M, Hanada K, Saijo M, Fukasawa M. Structural basis of antiviral activity of caffeic acid against severe fever with thrombocytopenia syndrome virus. J Infect Chemother. 2021 Feb;27(2):397-400. doi: 10.1016/j.jiac.2020.10.015. Epub 2020 Oct 24. PMID: 3310943
5. Tanida I, Kakuta S, Oliva Trejo JA, Uchiyama Y. Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein. Sci Rep. 2020 Jul 9;10(1):11314. doi: 10.1038/s41598-020-68191-z. PMID: 32647231
6. Oliva Trejo JA, Tanida I, Suzuki C, Kakuta S, Tada N, Uchiyama Y. Characterization of starvation-induced autophagy in cerebellar Purkinje cells of pHluorin-mKate2-human LC3B transgenic mice. Sci Rep. 2020 Jun 15;10(1):9643. doi: 10.1038/s41598-020-66370-6. PMID: 32541814; PMCID: PMC7295967.
7. Morimoto K, Suzuki N, Tanida I, Kakuta S, Furuta Y, Uchiyama Y, Hanada K, Suzuki Y, Yamaji T: Blood group P1 antigen-bearing glycoproteins are functional but less efficient receptors of Shiga toxin than conventional glycolipid-based receptors. J Biol Chem. 2020, jbc.RA120.013926. doi: 10.1074/jbc.RA120.013926.
8. Ogawa M, Takada N, Shizukuishi S, Tomokiyo M, Chang B, Yoshida M, Kakuta S, Tanida I, Ryo A, Guan JL, Takeyama H, Ohnishi M: Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization. Commun Biol. 2020, 3:25. doi: 10.1038/s42003-020-0753-3. 3
9. Suzuki C, Tanida I, Oliva Trejo JA, Kakuta S, Uchiyama Y: Autophagy Deficiency in Renal Proximal Tubular Cells Leads to an Increase in Cellular Injury and Apoptosis under Normal Fed Conditions. Int J Mol Sci. 2019, 21:155. doi: 10.3390/ijms21010155.
10. Okemoto-Nakamura Y, Tanida I, Yamaji T, Hanada K, and Hagiwara K: A PRNP-disrupted human neuroblastoma cell line and its stable transformants expressing prion protein variants. BPB reports, 2019, 52:357-65. doi: 10.1111/j.1348-0421.2008.00046.x.
11. Yazawa R, Nishida Y, Aoyama S, Tanida I, Miyatsuka T, Suzuki L, Himuro M, Haruna H, Takubo N, Shimizu T, Watada H: Establishment of a system for screening autophagic flux regulators using a modified fluorescent reporter and CRISPR/Cas9. Biochem Biophys Res Commun. 516:686-692, 2019
12. Sou YS, Kakuta S, Kamikubo Y, Niisato K, Sakurai T, Parajuli LK, Tanida I, Saito H, Suzuki N, Sakimura K, Maeda Y, Kinoshita T, Uchiyama Y, Koike M: Cerebellar Neurodegeneration and Neuronal Circuit Remodeling in Golgi pH Regulator-Deficient Mice. eNeuro. 6. pii: ENEURO.0427-18.2019
13. Shirasago Y, Inamori Y, Suzuki T, Tanida I, Suzuki T, Sugiyama K, Wakita T, Hanada K, Fukasawa M: Inhibition Mechanisms of Hepatitis C Virus Infection by Caffeic Acid and Tannic Acid. Biol Pharm Bull. 42:770-777, 2019
14. Suzuki C, Tanida I, Ohmuraya M, Oliva Trejo JA, Kakuta S, Sunabori T, Uchiyama Y. Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury. Int J Mol Sci. 5;20. pii: E1711, 2019
15. Nitta A, Hori K, Tanida I, Igarashi A, Deyama Y, Ueno T, Kominami E, Sugai M, Aoki K: Blocking LC3 lipidation and ATG12 conjugation reactions by ATG7 mutant protein containing C572S. Biochem Biophys Res Commun. 508:521-526, 2018
16. Ogawa M, Matsuda R, Takada N, Tomokiyo M, Yamamoto S, Shizukusihi S, Yamaji T, Yoshikawa Y, Yoshida M, Tanida I, Koike M, Murai M, Morita H, Takeyama H, Ryo A, Guan JL, Yamamoto M, Inoue JI, Yanagawa T, Fukuda M, Kawabe H, Ohnishi M: Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1-mediated K63-linked ubiquitination. Cell Microbiol. 20:e12846, 2018
17. Tanida-Miyake E, Koike M, Uchiyama Y, Tanida I: Optimization of mNeonGreen for Homo sapiens increases its fluorescent intensity in mammalian cells. PLoS ONE. 13 :e0191108, 2018
18. Yamaguchi J, Suzuki C, Nanao T, Kakuta S, Ozawa K, Tanida I, Saitoh T, Sunabori T, Komatsu M, Tanaka K, Aoki S, Sakimura K, Uchiyama Y: Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis. Autophagy. 14:764-777, 2018
19. Tanida I, Ueno T, Uchiyama Y: Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses. Methods Enzymol. 587:87-96, 2017
20. Shirasago Y, Shimizu Y, Tanida I, Suzuki T, Suzuki R, Sugiyama K, Wakita T, Hanada K, Yagi K, Kondoh M, Fukasawa M: Occludin-Knockout Human Hepatic Huh7.5.1-8-Derived Cells Are Completely Resistant to Hepatitis C Virus Infection. Biol Pharm Bull. 39:839-48, 2016
21. Klionsky DJ, Tanida I, et al. (2467人中2071番): Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12:1-222, 2016
22. Rinchai D, Riyapa D, Buddhisa S, Utispan K, Titball RW, Stevens MP, Stevens JM, Ogawa M, Tanida I, Koike M, Uchiyama Y, Ato M, Lertmemongkolchai G: Macroautophagy is essential for killing of intracellular Burkholderia pseudomallei in human neutrophils. Autophagy. 11: 784-755, 2015
23. Tanida I, Shirasago Y, Suzuki R, Abe R, Wakita T, Hanada K, Fukasawa M: Caffeic acid, a coffee-related organic acid, inhibits the propagation of hepatitis C virus. Jpn J Infect Dis. 68: 268-275, 2014
24. Tanida I, Ueno T, Uchiyama Y: A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian. Autophagy PLoS ONE. 9: e110600, 2014
25. Harada-Hada K, Harada K, Kato F, Hisatsune J, Tanida I, Ogawa M, Asano S, Sugai M, Hirata M, Kanematsu T.: Phospholipase C-related catalytically inactive protein participates in the autophagic elimination of Staphylococcus aureus infecting mouse embryonic fibroblasts. PLoS ONE. 9:e98285, 2014
26. Asano S, Nemoto T, Kitayama T, Harada K, Zhang J, Harada K, Tanida I, Hirata M, Kanematsu T: Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion. Biol Open 3:463-74, 2014
27. Tanida I*, Koike M*, Nanao T, Tada N, Iwata JI, Ueno T, Kominami E, Uchiyama Y: Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons. PLoS one 8: e63568, 2013 (*: equally contributed)
28. Umebayashi H, Mizokami A, Matsuda M, Harada K, Takeuchi H, Tanida I, Hirata M, Kanematsu T: Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation. Biochem Biophys Res Commun. 432: 268-74, 2013
29. Tanida I, Yamasaki M, Komatsu M, Ueno T: The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation. Autophagy 8:88-97, 2012
30. Klionsky DJ, Tanida I, et al. (1204人中1082番): Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8: 445-544, 2012
31. Takagi-Akiba M, Asanuma K, Tanida I, Tada N, Oliva Trejo JA, Nonaka K, Asanuma E, Kominami E, Ueno T, Tomino Y: Doxorubicin-induced glomerulosclerosis with proteinuria in GFP-GABARAP transgenic mice. Am J Physiol Renal Physiol 302:F380-9, 2012
32. Inami Y, Yamashina S, Izumi K, Ueno T, Tanida I, Ikejima K, Watanabe S: Hepatic steatosis inhibits autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression. Biochem Biophys Res Commun 412: 618-25, 2011
33. Ezaki J, Matsumoto N, Takeda-Ezaki M, Komatsu M, Takahashi K, Hiraoka Y, Taka H, Fujimura T, Takehana K, Yoshida M, Iwata J, Tanida I, Furuya N, Zheng DM, Tada N, Tanaka K, Kominami E, Ueno T: Liver autophagy contributes to the maintenance of blood glucose and amino acid levels. Autophagy. 7: 727-736, 2011
34. Saiki S, Sasazawa Y, Imamichi Y, Kawajiri S, Fujimaki T, Tanida I, Kobayashi H, Sato F, Sato S, Ishikawa K, Imoto M, Hattori N: Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7: 176-87, 2011
35. Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T: Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol 183: 7278-7285, 2009
36. Tanida I, Fukasawa M, Ueno T, Kominami E, Wakita T, Hanada K: Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles. Autophagy 5: 937-945, 2009
37. Mizui T, Yamashina S, Tanida I, Takei Y, Ueno T, Sakamoto N, Ikejima K, Kitamura T, Enomoto N, Sakai T, Kominami E, Watanabe S: Inhibition of hepatitis C virus replication by chloroquine targeting virus-associated autophagy. J Gastroenterol 45: 195-203, 2009
38. Takahashi K, Ueno T, Tanida I, Minematsu-Ikeguchi N, Murata M, Kominami E.; Characterization of CAA0225, a novel inhibitor specific for cathepsin L, as a probe for autophagic proteolysis. Biol Pharm Bull 32: 475-9, 2009
39. Haruna K, Suga Y, Muramatsu S, Taneda K, Mizuno Y, Ikeda S, Ueno T, Kominami E, Tanida I, Hanada K: Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes. J Dermatol Sci 52: 213-215, 2008
40. Okemoto-Nakamura Y, Yamakawa Y, Hanada K, Tanaka K, Miura M, Tanida I, Nishijima M, Hagiwara K: A Synthetic Fibril Peptide Promotes Clearance of Scrapie Prion Protein by Lysosomal Degradation. Microbiol Immunol 52: 357-365, 2008
41. Ueno T, Sato W, Horie Y, Komatsu M, Tanida I, Yoshida M, Ohshima S, Mak TW, Watanabe S, Kominami E: Loss of Pten, a tumor suppressor, causes the strong inhibition of autophagy without affecting LC3 lipidation. Autophagy 4: 692-700, 2008
42. Klionsky DJ, Tanida I, et al. (236人中202番): Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4: 151-175, 2008
43. Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K: Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Autophagy 4: 131-134. 2008
44. Uchio N, Oma Y, Toriumi K, Sasagawa N, Tanida I, Fujita E, Kouroku Y, Kuroda R, Momoi T, Ishiura S: Endoplasmic reticulum stress caused by aggregate-prone proteins containing homopolymeric amino acids. FEBS J 274: 5619-27, 2007
45. Mizokami A, Kanematsu T, Ishibashi H, Yamaguchi T, Tanida I, Takenaka K, Nakayama KI, Fukami K, Takenawa T, Kominami E, Moss SJ, Yamamoto T, Nabekura J, Hirata M: Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABAA receptors to the cell surface. J Neurosci 27: 1692-1701, 2007
46. Kouroku Y, Fujita E, Tanida I, Ueno T, Isoai A, Kumagai H, Ogawa S, Kaufman RJ, Kominami E, Momoi T: ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation. Cell Death Differ 14: 230-239, 2007
47. Demarchi F, Bertoli C, Copetti T, Tanida I, Brancolini C, Eskelinen EL, Schneider C.; Calpain is required for macroautophagy in mammalian cells. J Cell Biol. 175: 595-605, 2006
48. Tanida I, Wakabayashi M, Kanematsu T, Minematsu-Ikeguchi N, Sou YS, Hirata M, Ueno T, Kominami E: Lysosomal turnover of GABARAP-phospholipid conjugate is activated during differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. Autophagy 2: 264-271, 2006
49. Tanida I, Sou YS, Minematsu-Ikeguchi N, Ueno T, Kominami E: Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3. FEBS J 273: 2553-2562, 2006
50. Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, Ueno T, Koike M, Uchiyama Y, Kominami, E, Tanaka K: Loss of autophagy in the central nervous system causes neurodegeneration. Nature 441: 880-884, 2006
51. Sou Y, Tanida I, Komatsu M, Ueno T, Kominami E: Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP and GATE-16. J Biol Chem 281: 3017-3024, 2006
52. Iwata J, Ezaki J, Komatsu M, Yokota S, Ueno T, Tanida I, Chiba T, Tanaka K, Kominami E: Excess peroxisomes are degraded by autophagic machinery in mammals. J Biol Chem 281: 4035-4041, 2006
53. Kumanomidou T, Mizushima T, Komatsu M, Suzuki A, Tanida I, Sou Y, Ueno T, Kominami E, Tanaka K, Yamane T.; The Crystal Structure of Human Atg4b, a Processing and De-conjugating Enzyme for Autophagosome-forming Modifiers. J Mol Biol 355: 612-618, 2005
54. Koike M, Shibata M, Waguri S, Tanida I, Kominami E, Gotow T, Peters C, von Figura K, Mizushima N, Saftig P, Uchiyama Y: Participation of autophagy in storage of lysosomes in neurons from mouse model of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol 167: 1713-1728, 2005
55. Tanida I, Minematsu-Ikeguchi N, Ueno T, Kominami E: Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1: 84-91, 2005
56. Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T: Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 169: 425-434, 2005
57. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kanematsu T, Mori Y, Shinoda H, Hirata M, Kominami E, Kawano K.: Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains. J Biol Chem 280: 24610-24617, 2005
58. Tanida I, Ueno T, Kominami E: Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem 279: 47704-47710, 2004
59. Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K: A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J 23: 1977-1986, 2004
60. Tanida I, Sou YS, Ezaki J, Minematsu-Ikeguchi N, Ueno T, Kominami E: HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates. J Biol Chem 279: 36268-36276, 2004
61. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kominami E, Kawano K: 1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3. J Biomol NMR 29: 415-416, 2004
62. Jäger S, Bucci C, Tanida I, Ueno T, Kominami E, Saftig P, Eskelinen EL: Role for Rab7 in maturation of late autophagic vacuoles. J Cell Sci 117: 4837-4848, 2004
63. Asanuma K, Tanida I, Shirato I, Ueno T, Takahara H, Nishitani T, Kominami E, Tomino Y: MAP-LC3, a promising autophagosomal marker, is processed during the differentiation and recovery of podocytes from PAN nephrosis. FASEB J 17: 1165-1167, 2003
64. Nemoto T, Tanida I, Tanida-Miyake E, Minematsu-Ikeguchi N, Yokota M, Ohsumi M, Ueno T, Kominami E: The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification. J Biol Chem 278: 39517-39526, 2003
65. Yamazaki-Sato H, Tanida I, Ueno T, Kominami E: The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett 551: 71-77, 2003
66. Tanida I, Komatsu M, Ueno T, Kominami E: GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3. Biochem Biophys Res Commun 300: 637-644, 2003
67. Tanida I, Tanida-Miyake E, Komatsu M, Ueno T, Kominami E: Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J Biol Chem 277: 13739-13744, 2002
68. Tanida I, Nishitani T, Nemoto T, Ueno T, Kominami E: Mammalian Apg12p, but not the Apg12p-Apg5p conjugate, facilitates LC3 processing. Biochem Biophys Res Commun 296: 1164-1170, 2002
69. Tanida I, Tanida-Miyake E, Nishitani T, Komatsu M, Yamazaki H, Ueno T, Kominami E: Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs. Biochem Biophys Res Commun 292: 256-262, 2002
70. Komatsu M, Tanida I, Ueno T, Ohsumi M, Ohsumi Y, Kominami E: The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation. J Biol Chem 276: 9846-9854, 2001
71. Tanida I, Tanida-Miyake E, Ueno T, Kominami E: The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J Biol Chem 276: 1701-1706, 2001
72. Ichimura Y, Kirisako T, Takao T, Satomi Y, Shimonishi Y, Ishihara N, Mizushima N, Tanida I, Kominami E, Ohsumi M, Noda T, Ohsumi Y.: A ubiquitin-like system mediates protein lipidation. Nature 408: 488-492, 2000
73. Ezaki J, Tanida I, Kanehagi N, Kominami E: A lysosomal proteinase, the late infantile neuronal ceroid lipofuscinosis gene (CLN2) product, is essential for degradation of a hydrophobic protein, the subunit c of ATP synthase. J Neurochem: 72, 2573-2582, 1999
74. Ueno T, Ishidoh K, Mineki R, Tanida I, Murayama K, Kadowaki M, Kominami E: Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. J Biol Chem 274: 15222-15229, 1999
75. Tanida I, Mizushima N, Kiyooka M, Ohsumi M, Ueno T, Ohsumi Y, Kominami E: Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy. Mol Biol Cell 10: 1367-1379, 1999
76. Tanida I, Takita Y, Hasegawa A, Ohya Y, Anraku Y: Yeast Cls2p/Csg2p localized on the endoplasmic reticulum membrane regulates a non-exchangeable intracellular Ca2+ pool cooperatively with calcineurin. FEBS Lett 379: 38-42, 1996
77. Tanida I, Hasegawa A, Iida H, Ohya Y, Anraku Y: Cooperation of calcineurin and vacuolar H+-ATPase in intracellular Ca2+ homeostasis of yeast cells. J Biol Chem 270: 10113-10119, 1995
78. Yagi S, Yanagida M, Tanida I, Hasegawa A, Okumura K, Ra C: High-level expression of the truncated alpha chain of human high-affinity receptor for IgE as a soluble form by baculovirus-infected insect cells. Biochemical characterization of the recombinant product. Eur J Biochem 220: 593-598, 1994
79. Tanida I, Yanagida M, Maki N, Yagi S, Namiyama F, Kobayashi T, Hayano T, Takahashi N, Suzuki M: Yeast cyclophilin-related gene encodes a nonessential second peptidyl-prolyl cis-trans isomerase associated with the secretory pathway. Transplant Proc 23: 2856-2861, 1991
80. Ohya Y, Umemoto N, Tanida I, Ohta A, Iida H, Anraku Y: Calcium-sensitive cls mutants of Saccharomyces cerevisiae showing a Pet- phenotype are ascribable to defects of vacuolar membrane H+-ATPase activity. J Biol Chem 266: 13971-13977, 1991
81. Galons JP, Tanida I, Ohya Y, Anraku Y, Arata Y: A multinuclear magnetic resonance study of a cls11 mutant showing the Pet- phenotype of Saccharomyces cerevisiae. Eur J Biochem 193: 111-119, 1990

「英文総説」
1. Tanida I: Autophagosome formation and molecular mechanism of autophagy. Antioxid Redox Signal 14: 2201-14, 2011
2. Tanida I: Autophagy basics. Microbiol Immunol 55: 1-11, 2011
3. Tanida I, Ueno T, Kominami E: LC3 conjugation system in mammalian autophagy. Int J Biochem Cell Biol 36: 2503-2518, 2004

「和文総説」
1. 谷田以誠: C型肝炎とオートファジー、J. Gastrointest. Research 2015.23: 205-211
2. 谷田以誠：オートファジー-リソソーム分解系～疾患・生理機能との関わりと分子機構、内分泌・糖尿病・代謝内科 33: 319-325, 2011
3. 谷田以誠、上野隆、和栗聡：オートファジーの評価方法、実験医学 29: 2054-2062, 2011
4. 谷田以誠：哺乳類ATG結合系、蛋白質核酸酵素 10: 1490-1493, 2006
5. 谷田以誠：哺乳類オートファジーとATGコンジュゲーションシステム、医学のあゆみ 216: 505-508, 2006
6. 谷田以誠：Atg7/Apg7ヒトホモログ、生体の科学 55: 424-425, 2004
7. 谷田以誠：腎障害とLC3ユビキチン様修飾、実験医学 22: 311-318, 2003
8. 谷田以誠、木南英紀：オートファジーにおけるApg7タンパク質活性化酵素、生体の科学 54: 521-527, 2003
9. 谷田以誠：酵母のオートファジータンパク質Apg7pとヒトホモログ、生体の科学 52: 612,2001
10. 木南英紀、江崎淳二、谷田以誠：プロテオリピッドの分解とリソソーム、生体の科学 50: 140-144, 1999

「英文著書」
1. Tanida I, and Kioke M: TECHNIQUES FOR STUDYING AUTOPHAGY in Autophagy, Infection, and the Immune Response, Jackson W, & Swanson M ed., Wiley Blackwell, John Wiley and Sons, Inc., Hoboken, NJ, 19-32, 2014
2. Tanida I, Ueno T, Kominami E: In Vitro Assays of Lipidation of Mammalian Atg8 Homologs. Current Protocols in Cell Biology, 11.20. 1-11.20. 13, 2014
3. Tanida I, Waguri S: Measurement of autophagy in cells and tissues. Methods Mol Biol 648: 193-214, 2010
4. Tanida I, Ueno T, Kominami E: LC3 and autophagy. Methods Mol Biol 445: 77-88, 2008
5. Ueno T, Tanida I, Kominami E: Autophagy and neuromuscular diseases in Autophagy, Klionsky D, ed., Landes Bioscience, Georgetown, Texas, 264-286, 2004

「和文著書」
1. 淺沼克彦、谷田以誠: オートファジー評価法，腎臓病実験法ハンドブック，編者富野康日己，中外医学社，東京都新宿区，19-24, 2013
2. 谷田以誠: 遺伝子組み替え抗原とその免疫測定への応用，バイオ検査薬と機器・装置，編者山本重夫，シーエムシー，東京都千代田区，106-115，1996

「英文報告その他」
　招待講演
1. Tanida I: Autophagy and Infection, Hua Hin 2013 Meeting, Hua Hin, Thailand, 2013
2. Tanida I: Autophagic Mechanism and Production of HCV Particles, World Congress of Virus and Infection-2010. Busan, Korea, 2010
3. Tanida I: Autophagy in naive hepatitis C virus production, 5th International Symposium on Autophagy. Shiga, Japan, 2009
4. Tanida I: Autophagy basics: International Symposium on Autophagy and Cell Death. Tokyo, Japan, 2009
5. Tanida I, Komatsu M, Iwata JI, Ezaki J, Ueno T, Kominami E: Mammalian Atg-conjugation systems: Their molecular and physiological functions. 19th Annual & International Meeting of the Japanese Association for Animal Cell Technology. Kyoto, Japan, 2006
6. Tanida I: The lysosomal turnover, but not a transient amount, of endogenous LC3 is a marker for autophagy. Gordon Research Conferences "Autophay in Stress, Development and Disease". Il Ciocco, Barga, Italy, 2005
7. Tanida I: Biochemistry of mammalian Apg genes. Gordon Research Conferences "Autophay in Stress, Development and Disease". Waterville, Maine, USA, 2003
8. Tanida I: Crosstalk between two ubiquitin-like modifications. III International Symposium on Autophagy. Osaka, 2002

インタビュー
1. Tanida I: SCIENCE WATCH INTERVIEW, THOMSON REUTERS・ SCIENCE WATCH・Special Topic of Autophagy
http://archive.sciencewatch.com/ana/st/autophagy/09octSTAutoTani.pdf

「和文報告その他」
シンポジウム企画・講演
1. 谷田以誠、甲賀大輔：シンポジウム「先端的電顕技術が切り拓くオルガネライメージングの世界」、第125回日本解剖学会総会全国学術集会、新潟2020
2. 谷田以誠、甲賀大輔：シンポジウム「細胞内スーパーイメージングの最先端」、第124回日本解剖学会総会全国学術集会、新潟2019
3. 谷田以誠、甲賀大輔：ワークショップ「細胞内スーパーイメージングの世界」、第124回日本分子生物学会、横浜2018
4. 谷田以誠、新崎恒平：「病原体感染におけるオルガネラ・ダイナミクス」シンポジウム、第87回日本生化学会大会、京都2014

　特許出願
1. 酵母プロテインジスルフィドイソメラ−ゼ　平成　２年
　（特願平2-322620）
2. 昆虫細胞による可溶性ＩｇＥ結合受容体α鎖の製造方法　平成　５年
　（特願平05-201991、公開番号:特開平07-031483、
　平成１５年成立登録番号：3473973号）
3. 酵母により可溶性ＩｇＥ結合受容体α鎖を製造する方法　平成　４年
　（特願平4-308896、公開番号：特開平06-153967）
4. ＩｇＥの測定方法　平成５年
　（特願平05-218920、公開番号：特開平07-072150）
5. 免疫抑制物質のスクリーニング方法　平成　６年
　（特願平06-116622、公開番号：特開平07-322891）
6. アレルギー反応誘発性ＩｇＥの特異的測定方法　平成　６年
　（特願平06-217469、公開番号：特開平08-082592）
7. 免疫抑制物質のスクリーニング方法　平成　６年
　（特願平06-116731、公開番号：特開平07-322982）
8. 免疫抑制物質のスクリーニング方法　平成　６年
　（特願平06-116622、公開番号：特開平07-322891）
9. 免疫抑制剤高感受性変異株及びその使用　平成　７年
　（特願平07-341819、公開番号：特開09-173053）
10. トランスジェニック非ヒト動物　平成１８年
　（特願2006-284583、公開番号：特開2008-99601）
11. トランスジェニック非ヒト動物　平成１８年
　（特願2006-284568、公開番号：特開2008-99600）　
12. ATG７変異体を用いたオートファジーの抑制方法　平成２４年
　（特願2012-280082）　

インタビュー
1. 内山　安男、上野　隆、谷田　以誠: ノーベル医学・生理学賞 “オートファジー”どう影響？、首都圏ネットワーク・NHK総合テレビ午後6時から

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谷田 以誠（タニダ イセイ）

谷田以誠（タニダイセイ）