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桑原京子（クワハラキョウコ）

研究テーマ	細菌学　薬剤耐性　黄色ブドウ球菌
研究業績（論文）	1. Antimicrobial cathelicidin peptide LL-37 inhibits the pyroptosis of macrophages and improves the survival of polybacterial septic mice. Hu Z, Murakami T, Suzuki K, Tamura H, Reich J, Kuwahara-Arai K, Iba T, Nagaoka I. Int Immunol. 2016 May;28(5):245-53. 2. Human Host Defense Cathelicidin Peptide LL-37 Enhances the Lipopolysaccharide Uptake by Liver Sinusoidal Endothelial Cells without Cell Activation. Suzuki K, Murakami T, Hu Z, Tamura H, Kuwahara-Arai K, Iba T, Nagaoka I. J Immunol. 2016 Feb 1;196(3):1338-47. 3.Complete Genome Sequence of Methicillin-Resistant Staphylococcus schleiferi Strain TSCC54 of Canine Origin. Sasaki T, Tsubakishita S, Kuwahara-Arai K, Matsuo M, Lu YJ, Tanaka Y, Hiramatsu K. Genome Announc. 2015 Oct 29;3(5). pii: e01268-15. doi: 10.1128/genomeA.01268-15. 4. A mutation of RNA polymerase β' subunit (RpoC) converts heterogeneously vancomycin-intermediate Staphylococcus aureus (hVISA) into "slow VISA". Matsuo M, Hishinuma T, Katayama Y, Hiramatsu K. Antimicrob Agents Chemother. 2015 Jul;59(7):4215-25. d 5. "Slow VISA," a novel phenotype of vancomycin resistance, found in vitro in heterogeneous vancomycin-intermediate Staphylococcus aureus strain Mu3. Saito M, Katayama Y, Hishinuma T, Iwamoto A, Aiba Y, Kuwahara-Arai K, Cui L, Matsuo M, Aritaka N, Hiramatsu K. Antimicrob Agents Chemother. 2014 Sep;58(9):5024-35. 6. Antimicrobial cathelicidin peptide LL-37 inhibits the LPS/ATP-induced pyroptosis of macrophages by dual mechanism. Hu Z, Murakami T, Suzuki K, Tamura H, Kuwahara-Arai K, Iba T, Nagaoka I. PLoS One. 2014 Jan 16;9(1):e85765. 7. Genomic Basis for Methicillin Resistance in Staphylococcus aureus. Hiramatsu K, Ito T, Tsubakishita S, Sasaki T, Takeuchi F, Morimoto Y, Katayama Y, Matsuo M, Kuwahara-Arai K, Hishinuma T, Baba T. Infect Chemother. 2013 Jun;45(2):117-36. 8. Staphylococcal Cassette Chromosome mec (SCCmec) analysis of MRSA. Ito T, Kuwahara-Arai K, Katayama Y, Uehara Y, Han X, Kondo Y, Hiramatsu K. Methods Mol Biol. 2014;1085:131-48. 9. Investigation of nasal meticillin-resistant Staphylococcus aureus carriage in a haemodialysis clinic in Japan. Uehara Y, Kuwahara-Arai K, Hori S, Kikuchi K, Yanai M, Hiramatsu K. J Hosp Infect. 2013 May;84(1):81-4. 10. Rapid and accurate identification of human-associated staphylococci by use of multiplex PCR. Hirotaki S, Sasaki T, Kuwahara-Arai K, Hiramatsu K. J Clin Microbiol. 2011 Oct;49(10):3627-31. 11. Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells. Suzuki K, Murakami T, Kuwahara-Arai K, Tamura H, Hiramatsu K, Nagaoka I. Int Immunol. 2011 Mar;23(3):185-93. 12. Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. Kaito C, Saito Y, Nagano G, Ikuo M, Omae Y, Hanada Y, Han X, Kuwahara-Arai K, Hishinuma T, Baba T, Ito T, Hiramatsu K, Sekimizu K. PLoS Pathog. 2011 Feb 3;7(2):e1001267. 13.Origin and molecular evolution of the determinant of methicillin-resistance in staphylococci. Tsubakishita S, Kuwahara-Arai K, Sasaki T, Hiramatsu K. Antimicrob Agents Chemother. 2010 Aug 2. 14. Antimicrobial cathelicidin polypeptide CAP11 suppresses the production and release of septic mediators in D-galactosamine-sensitized endotoxin shock mice. Murakami T, Obata T, Kuwahara-Arai K, Tamura H, Hiramatsu K, Nagaoka I. Int Immunol. 2009 Aug;21(8):905-12. Epub 2009 Jun 25. 15. Augmentation of the antimicrobial activities of guinea pig cathelicidin CAP11-derived peptides by amino acid substitutions. Okuda D, Yomogida S, Kuwahara-Arai K, Hitamatsu K, Tamura H, Nagaoka I. Int J Mol Med. 2009 Apr;23(4):501-8. 16. Complete Genome Sequence of Macrococcus caseolyticus Strain JSCS5402, Reflecting the Ancestral Genome of the Human-Pathogenic Staphylococci Tadashi Baba, Kyoko Kuwahara-Arai, Ikuo Uchiyama, Fumihiko Takeuchi, Teruyo Ito, and Keiichi Hiramatsu J Bacteriol. 2009 February; 191(4): 1180–1190. 17. Extreme Genetic Diversity of Methicillin-Resistant Staphylococcus epidermidis Strains Disseminated among Healthy Japanese Children Tengku Zetty Maztura Tengku Jamaluddin, Kyoko Kuwahara-Arai, Ken Hisata, Masahiko Terasawa, Longzhu Cui, Tadashi Baba, Chie Sotozono, Shigeru Kinoshita, Teruyo Ito, and Keiichi Hiramatsu J Clin Microbiol. 2008 November; 46(11): 3778–3783. 18. Antibacterial cathelicidin peptide CAP11 suppresses the anandamide production from lipopolysaccharide-stimulated mononuclear phagocytes. Murakami T, Yomogida S, Someya A, Kuwahara-Arai K, Tamura H, Nagaoka I. FEBS Lett. 2007 Jan 9;581(1):140-4. 19.Kimura H., Mukaida M., Kuwahara K, Ito T, Hashino K, Uchida K, Matsumoto K, Yoshida K. 4-Hydroxynonenal modifies IgA in rat intestine after lipopolysaccharide injection. Free Radic. Biol. Med. 41: 973-978. 2006 20. Nagaoka I, K. kuwahara-Arai, H. Tamura, K. Hiramatsu and M. Hirata. Augmentation of the bactericidal activities of human cathelicidin CAP18/LL-37-derived antimicrobial peptides by amino acid substitutions. Inflamm. Res. 54,66-73. 2005 21.Hisata K, Kuwahara-Arai K, Yamanoto M, Ito T, Nakatomi Y, Cui L, Baba T, Terasawa M, Sotozono C, Kinoshita S, Yamashiro Y, Hiramatsu K. Dissemination of methicillin-resistant staphylococci among healthy Japanese children. J Clin Microbiol. 2005 Jul;43(7):3364-72 22.Ito T, Kuwahara K, Hisata K, Okuma K, Cui L, Hiramatsu K Community-associated methicillin-resistant Staphylococcus aureus: current status and molecular epidemiological perspective. Kansenshogaku Zasshi. 2004 Jun;78(6):459-69. 23.Reiko Yoshida, Kyoko Kuwahara-Arai, Tadashi Baba,Longzhu Cui, Judith F. Richardson, and Keiichi Hiramatsu. Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance. in press. 24.Noriko Kondo, Kyoko kuwahara-arai, Hiroko Kuroda-Murakami, Eiko Tateda-Suzuki, and Keiichi Hiramatsu,Eagle-type methicillin resistance : new phenotype of high methicillin resistance under mec regulator gene control. 2001. Antimicrob. Chemother. 45 : 815-824 25.Kuroda M., Kuwahra-Arai K., Hiramatsu K. Identification of the up- and down -regulated genes in vancomycin-resisant staphylococcus aureus strain Mu3 and Mu50 by cDNA differential hybridization method. Bochemical and Biophysical Research Communications . 269,485-490,2000 26.Cui L., Murakami H., Kuwahara-Arai K., Hanaki H., Hiramatsu H.: Contribution of a thickened cell wall and its glutamine nonamidated component to the vancomycin resistance expressed by Staphylococcus aureus Mu50. Antimicrob. Agents Chemother.44:2276-22285,2000 27.Fujisawa M., Kojima K., Beppu T., Futagawa S., Kuwahara K., Hiramatsu K. : Early diagnosis of postoperative infection : assessment of whole blood chemiluminescence. Surg Today. 30:309-318, 2000 28.舘田映子、近藤典子、桑原京子、堀　賢、平松啓一、横田健、Gatifloxacinの細菌学的検討。1999. 日本化学療法学会雑誌　47(S-2) 48-56 29.Murakmi H., Kuwahara-Arai K., Tsuchihashi H., Hnaki H., Hiramatsu K. : Overoriduction of PBP2 is associated with vancomycin and teicoplanin- resistance in staphylococcus aureus.J Antimicrob.Chemother.44 Suppl. A. :167,1999. 30.Kuwahara-Arai K., Yoshida R., Hiramatsu K. : Overproduction of penicillin-binding protein 1 and 2 in a mecA-negative methicillin-resistant staphylococcus aureus clinical srain 61/5896. J Antimicrob.Chemother.44 Suppl. A. :167,1999. 31.Akihiko Ohwada,Mitsuaki Sekiya, Hideaki Hanaki, Kyoko Kuwahara-Arai,Isao Nagaoka,Satoshi Hori, Shigeru Tominaga Keiichi Hiramatsu, and Yoshinosuke Fukuchi.DNA vactination by mecA sequence evokes an antibacter1ial immune response against methicillin-resistant Staphylococcus aureus. 1999. J Antimicrob. Chemother.44 : 767-774 32.Vancomycin に対するStaphylococcus aureus のヘテロ耐性メカニズムと簡易検出法の検討。稲葉陽子、花木秀明、高橋順子、杉本奈扶美、桑原京子、崔　　龍洙、平松啓一、1999.日本化学療法学会雑誌　47：339～344 33.H.Hanaki, K.Kuwahara -Arai, S.Boyle-Vavra,R.S.Daum, H.Labischinski , K.Hiramatsu. Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50. 1998. J Antimicrob.Chemother.42:199-209 34.H.Hanaki, H. Labischinski, K.Sasaki, K.Kuwahara-Arai, Y.Inaba, K.Hiramatsu.Mechanism of vancomycin resistance in MRSA strain Mu50. 1998.Jap J .Antibiotics. 51:237-247 著書 1)バンコマイシン耐性腸球菌。桑原京子、花木秀明、平松啓一、1999.微研ジャーナル友　江東微生物研究所：10～14 2)バンコマイシン耐性菌（VREとVRSA）感染症。桑原京子、花木秀明、平松啓一、1999.　感染症症候群I　日本臨床：182～185 3)新たな方向性：術後感染症の早期発見。藤沢稔、児島邦明、二川俊二、桑原京子、平松啓一、1998.耐性菌感染症の理論と実際　医薬ジャーナル社：169～173

研究テーマ

細菌学　薬剤耐性　黄色ブドウ球菌

研究業績（論文）

1. Antimicrobial cathelicidin peptide LL-37 inhibits the pyroptosis of macrophages and improves the survival of polybacterial septic mice.
Hu Z, Murakami T, Suzuki K, Tamura H, Reich J, Kuwahara-Arai K, Iba T, Nagaoka I.
Int Immunol. 2016 May;28(5):245-53.
2. Human Host Defense Cathelicidin Peptide LL-37 Enhances the Lipopolysaccharide Uptake by Liver Sinusoidal Endothelial Cells without Cell Activation.
Suzuki K, Murakami T, Hu Z, Tamura H, Kuwahara-Arai K, Iba T, Nagaoka I.
J Immunol. 2016 Feb 1;196(3):1338-47.
3.Complete Genome Sequence of Methicillin-Resistant Staphylococcus schleiferi Strain TSCC54 of Canine Origin.
Sasaki T, Tsubakishita S, Kuwahara-Arai K, Matsuo M, Lu YJ, Tanaka Y, Hiramatsu K.
Genome Announc. 2015 Oct 29;3(5). pii: e01268-15. doi: 10.1128/genomeA.01268-15.
4. A mutation of RNA polymerase β' subunit (RpoC) converts heterogeneously vancomycin-intermediate Staphylococcus aureus (hVISA) into "slow VISA".
Matsuo M, Hishinuma T, Katayama Y, Hiramatsu K.
Antimicrob Agents Chemother. 2015 Jul;59(7):4215-25. d
5. "Slow VISA," a novel phenotype of vancomycin resistance, found in vitro in heterogeneous vancomycin-intermediate Staphylococcus aureus strain Mu3.
Saito M, Katayama Y, Hishinuma T, Iwamoto A, Aiba Y, Kuwahara-Arai K, Cui L, Matsuo M, Aritaka N, Hiramatsu K.
Antimicrob Agents Chemother. 2014 Sep;58(9):5024-35.
6. Antimicrobial cathelicidin peptide LL-37 inhibits the LPS/ATP-induced pyroptosis of macrophages by dual mechanism.
Hu Z, Murakami T, Suzuki K, Tamura H, Kuwahara-Arai K, Iba T, Nagaoka I.
PLoS One. 2014 Jan 16;9(1):e85765.
7. Genomic Basis for Methicillin Resistance in Staphylococcus aureus.
Hiramatsu K, Ito T, Tsubakishita S, Sasaki T, Takeuchi F, Morimoto Y, Katayama Y, Matsuo M, Kuwahara-Arai K, Hishinuma T, Baba T.
Infect Chemother. 2013 Jun;45(2):117-36.
8. Staphylococcal Cassette Chromosome mec (SCCmec) analysis of MRSA.
Ito T, Kuwahara-Arai K, Katayama Y, Uehara Y, Han X, Kondo Y, Hiramatsu K.
Methods Mol Biol. 2014;1085:131-48.
9. Investigation of nasal meticillin-resistant Staphylococcus aureus carriage in a haemodialysis clinic in Japan.
Uehara Y, Kuwahara-Arai K, Hori S, Kikuchi K, Yanai M, Hiramatsu K.
J Hosp Infect. 2013 May;84(1):81-4.
10. Rapid and accurate identification of human-associated staphylococci by use of multiplex PCR.
Hirotaki S, Sasaki T, Kuwahara-Arai K, Hiramatsu K.
J Clin Microbiol. 2011 Oct;49(10):3627-31.
11. Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells.
Suzuki K, Murakami T, Kuwahara-Arai K, Tamura H, Hiramatsu K, Nagaoka I.
Int Immunol. 2011 Mar;23(3):185-93.
12. Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
Kaito C, Saito Y, Nagano G, Ikuo M, Omae Y, Hanada Y, Han X, Kuwahara-Arai K, Hishinuma T, Baba T, Ito T, Hiramatsu K, Sekimizu K.
PLoS Pathog. 2011 Feb 3;7(2):e1001267.
13.Origin and molecular evolution of the determinant of methicillin-resistance in staphylococci.
Tsubakishita S, Kuwahara-Arai K, Sasaki T, Hiramatsu K.
Antimicrob Agents Chemother. 2010 Aug 2.
14. Antimicrobial cathelicidin polypeptide CAP11 suppresses the production and release of septic mediators in D-galactosamine-sensitized endotoxin shock mice. Murakami T, Obata T, Kuwahara-Arai K, Tamura H, Hiramatsu K, Nagaoka I. Int Immunol. 2009 Aug;21(8):905-12. Epub 2009 Jun 25.
15. Augmentation of the antimicrobial activities of guinea pig cathelicidin CAP11-derived peptides by amino acid substitutions.
Okuda D, Yomogida S, Kuwahara-Arai K, Hitamatsu K, Tamura H, Nagaoka I.
Int J Mol Med. 2009 Apr;23(4):501-8.
16. Complete Genome Sequence of Macrococcus caseolyticus Strain JSCS5402, Reflecting the Ancestral Genome of the Human-Pathogenic Staphylococci
Tadashi Baba, Kyoko Kuwahara-Arai, Ikuo Uchiyama, Fumihiko Takeuchi, Teruyo Ito, and Keiichi Hiramatsu J Bacteriol. 2009 February; 191(4): 1180–1190.
17. Extreme Genetic Diversity of Methicillin-Resistant Staphylococcus epidermidis Strains Disseminated among Healthy Japanese Children
Tengku Zetty Maztura Tengku Jamaluddin, Kyoko Kuwahara-Arai, Ken Hisata, Masahiko Terasawa, Longzhu Cui, Tadashi Baba, Chie Sotozono, Shigeru Kinoshita, Teruyo Ito, and Keiichi Hiramatsu J Clin Microbiol. 2008 November; 46(11): 3778–3783.
18. Antibacterial cathelicidin peptide CAP11 suppresses the anandamide production from lipopolysaccharide-stimulated mononuclear phagocytes.
Murakami T, Yomogida S, Someya A, Kuwahara-Arai K, Tamura H, Nagaoka I.
FEBS Lett. 2007 Jan 9;581(1):140-4.
19.Kimura H., Mukaida M., Kuwahara K, Ito T, Hashino K, Uchida K, Matsumoto K, Yoshida K. 4-Hydroxynonenal modifies IgA in rat intestine after lipopolysaccharide injection. Free Radic. Biol. Med. 41: 973-978. 2006
20. Nagaoka I, K. kuwahara-Arai, H. Tamura, K. Hiramatsu and M. Hirata.
Augmentation of the bactericidal activities of human cathelicidin CAP18/LL-37-derived antimicrobial peptides by amino acid substitutions. Inflamm. Res. 54,66-73. 2005
21.Hisata K, Kuwahara-Arai K, Yamanoto M, Ito T, Nakatomi Y, Cui L, Baba T, Terasawa M, Sotozono C, Kinoshita S, Yamashiro Y, Hiramatsu K. Dissemination of methicillin-resistant staphylococci among healthy Japanese children. J Clin Microbiol. 2005 Jul;43(7):3364-72
22.Ito T, Kuwahara K, Hisata K, Okuma K, Cui L, Hiramatsu K
Community-associated methicillin-resistant Staphylococcus aureus: current status and molecular epidemiological perspective. Kansenshogaku Zasshi. 2004 Jun;78(6):459-69.
23.Reiko Yoshida, Kyoko Kuwahara-Arai, Tadashi Baba,Longzhu Cui, Judith F. Richardson, and Keiichi Hiramatsu. Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance. in press.
24.Noriko Kondo, Kyoko kuwahara-arai, Hiroko Kuroda-Murakami, Eiko Tateda-Suzuki, and Keiichi Hiramatsu,Eagle-type methicillin resistance : new phenotype of high methicillin resistance under mec regulator gene control. 2001. Antimicrob. Chemother. 45 : 815-824
25.Kuroda M., Kuwahra-Arai K., Hiramatsu K. Identification of the up- and down -regulated genes in vancomycin-resisant staphylococcus aureus strain Mu3 and Mu50 by cDNA differential hybridization method. Bochemical and Biophysical Research Communications . 269,485-490,2000
26.Cui L., Murakami H., Kuwahara-Arai K., Hanaki H., Hiramatsu H.: Contribution of a thickened cell wall and its glutamine nonamidated component to the vancomycin resistance expressed by Staphylococcus aureus Mu50. Antimicrob. Agents Chemother.44:2276-22285,2000
27.Fujisawa M., Kojima K., Beppu T., Futagawa S., Kuwahara K., Hiramatsu K. : Early diagnosis of postoperative infection : assessment of whole blood chemiluminescence. Surg Today. 30:309-318, 2000
28.舘田映子、近藤典子、桑原京子、堀　賢、平松啓一、横田健、Gatifloxacinの細菌学的検討。1999. 日本化学療法学会雑誌　47(S-2) 48-56
29.Murakmi H., Kuwahara-Arai K., Tsuchihashi H., Hnaki H., Hiramatsu K. : Overoriduction of PBP2 is associated with vancomycin and teicoplanin- resistance in staphylococcus aureus.J Antimicrob.Chemother.44 Suppl. A. :167,1999.
30.Kuwahara-Arai K., Yoshida R., Hiramatsu K. : Overproduction of penicillin-binding protein 1 and 2 in a mecA-negative methicillin-resistant staphylococcus aureus clinical srain 61/5896. J Antimicrob.Chemother.44 Suppl. A. :167,1999.
31.Akihiko Ohwada,Mitsuaki Sekiya, Hideaki Hanaki, Kyoko Kuwahara-Arai,Isao Nagaoka,Satoshi Hori, Shigeru Tominaga Keiichi Hiramatsu, and Yoshinosuke Fukuchi.DNA vactination by mecA sequence evokes an antibacter1ial immune response against methicillin-resistant Staphylococcus aureus. 1999. J Antimicrob. Chemother.44 : 767-774
32.Vancomycin に対するStaphylococcus aureus のヘテロ耐性メカニズムと簡易検出法の検討。稲葉陽子、花木秀明、高橋順子、杉本奈扶美、桑原京子、崔　　龍洙、平松啓一、1999.日本化学療法学会雑誌　47：339～344
33.H.Hanaki, K.Kuwahara -Arai, S.Boyle-Vavra,R.S.Daum, H.Labischinski , K.Hiramatsu. Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50. 1998. J Antimicrob.Chemother.42:199-209
34.H.Hanaki, H. Labischinski, K.Sasaki, K.Kuwahara-Arai, Y.Inaba, K.Hiramatsu.Mechanism of vancomycin resistance in MRSA strain Mu50. 1998.Jap J .Antibiotics. 51:237-247

著書
1)バンコマイシン耐性腸球菌。桑原京子、花木秀明、平松啓一、1999.微研ジャーナル友　江東微生物研究所：10～14
2)バンコマイシン耐性菌（VREとVRSA）感染症。桑原京子、花木秀明、平松啓一、1999.　感染症症候群I　日本臨床：182～185
3)新たな方向性：術後感染症の早期発見。藤沢稔、児島邦明、二川俊二、桑原京子、平松啓一、1998.耐性菌感染症の理論と実際　医薬ジャーナル社：169～173

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研究業績詳細

桑原 京子（クワハラ キョウコ）

桑原京子（クワハラキョウコ）