Hemorheology and Related Research Vol. 6, No. 2, pp. 83-88, 2003

Original Article

Effects of lemon polyphenols on serum lipoprotein profile, lipid peroxide production and hemorheological change in exercised hyperlipidemia rats.

Isao NAGAOKA1, Shin YOMOGIDA1, Hiroshi TOYOSHIMA1, Taro ISAYAMA2, Masanori HIRAMITSU3 and Hiroaki MIEDA3

1Department of Host Defense and Biochemical Research, Juntendo University, School of Medicine, 2Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, 3Pokka Corporation

Abstract : Polyphenols in citrus fruit have potent antioxidant capacities and exhibit potential health benefits for humans. In this study, to evaluate their protective effects on the exercised subjects with hyperlipidemia, lemon polyphenols containing hesperidin were adiministered to hypercholesterolemia rats, and lipid peroxide production and hemorheological change were assessed after running.
Male Sprague-Dawley rats weighing ca. 160 g were fed with high-cholesterol diet for 22 days. During the experimental period, rats were trained by tread-wheel running, and 0.08% hesperidin with 4.5% lemon juice was administered ad libitum. After the period, lipid peroxides, and HDL- and LDL-cholesterol were measured. Furthermore, hemorheological change (blood fluidity) was analyzed with MC-FAN, and TGF-β was quantitated by ELISA.
After running, the levels of lipid peroxides and TGF-β (a marker of fatigue) were elevated, and blood fluidity was retarded. Interestingly, lemon polyphenol-intake reversed these exercise-induced changes: lemon polyphenols reduced lipid peroxide and TGF-β levels, and improved blood fluidity. Furthermore, lemon polyphenols lowered LDL-cholesterol and increased HDL-cholesterol after running.
These observations suggest that lemon polyphenols containing hesperidin can exert a beneficial action on the exercised subjects with hyperlipidemia by reducing lipid peroxide production, and improving lipoprotein profile and blood fluidity.