Juntendo University, Tokyo, established in 1838.

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  4. Yoshihiko Araki M.D., D.Med.Sci.

Institute for Environmental and Gender-Specific Medicine

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Yoshihiko Araki M.D., D.Med.Sci.

M.D.: Yamagata University (1983)
D.Med.Sci.: Yamagata University (1987)
Postdoctral: Case Western Reserve University, Vanderbilt University

Academic appointments:

Assistant Professor, Department of Obstetrics & Gynecology
Yamagata University School of Medicine,
Associate Professor, Department of Immunology & Parasitology,
Yamagata University School of Medicine,
Research Associate Professor, Vanderbilt University School of Medicine,
Department of Obstetrics & Gynecology,
Associate Professor, Yamagata University School of Medicine,
Department of Immunology & Parasitology
Associate Professor, Juntendo University Graduate School of Medicine,
Institute for Environmental & Gender-specific Medicine
Adjunct Associate Professor, Juntendo University School of Medicine,
Department of Obstetrics & Gynecology

Office Address:

Institute for Environmental & Gender-specific Medicine,
Juntendo University Graduate School of Medicine
2-1-1 Tomioka, Urayasu-City 279-0021, Japan
Tel: 81-47-353-3171
Fax: 81-47-353-3178
E-mail: yaraki@med.juntendo.ac.jp


Hiroshi Yoshitake MD, PhD. Instructor
Shuichiro Endo M.D. Graduate student (Ph.D. course)

Research Keywords:

gametegenesis; fertilization; implantation, GPI-anchored protein

Research Speciality:

We are interested in control mechanism of gametegenesis, sperm-egg interaction, and implantation, i.e., fundamental biological process required for sexual reproduction.

Research Description:

Molecular mechanism of mammalian reproduction

  The research in our laboratory focuses on two areas of mammalian reproductive biology. First, our work has been directed toward understanding the molecular mechanism(s) of gametogenesis, especially sex difference during sperm/oogenesis. We identified a testicular protein (TEX101) predominantly expressed on the cell surface of spermatocytes and spermatids but not on the surface of Sertoli cells or interstitial cells, including Leydig cells, in adult mice. In developing testis, TEX101 is present on prospermatogonia in the immature seminiferous cords from 14 days post-coitus until 8 days post-partum. Thereafter, prospermatogonia arrange around the basal membranes of the seminiferous tubules and spermatogenesis is initiated. At this stage, TEX101 disappears from spermatogonia and is instead detected in spermatocytes and spermatids. Interestingly, sexually dimorphic expression of TEX101 is observed in developing gonadal tissues. For example, oogonia temporarily express TEX101 during the fetal stage, but the molecule is not detected in the sexually mature ovary. Using this molecule as a specific marker of the germ cell, we are currently investigating molecular regulation of male/female-specific gametegenesis as well as fertilization/implantation.
  A second area of interest is the microenvironment of the fertilization process in vivo. The mammalian fertilization process takes place in a complex microenvironment within the female genital tract. Although normal fertilization and pre-implantation development in vivo are possible without factors supplied from female genital tract, the significance of the microenvironment is no doubt essential for fertilization and early embryonic development. To evaluate physiological significance of the environment surrounded zygote in vivo, we are currently focused on immunological signal transduction during fertilization and early development at the site of fertilization, or oviduct.
  Our long-term goal would be to understand what is an essential difference between male and female. The information gained from studies described above will provide the framework to compare some aspects of male/female. Since most of living organizations are recognized as having ability for sexual reproduction, only hints to understand gender-specificity may exist as to how gametogenisis is regulated and what molecular mechanisms underlie this basic biological response.

Publications (since 2005 to date):

  1. Takayama T., Mishima T., Mori M., Jin H., Tsukamoto H., Takahashi K., Kinoshita K., Suzuki M., Sato I., Matsubara S., Araki Y., Takizawa T. Sexually dimorphic expression of the novel germ cell antigen TEX101 during mouse gonad development.
    Biol.Reprod. 72:1315-1323, 2005.
  2. Yoshitake H., Nitto T., Ohta N., Fukase S., Aoyagi M., Sendo F., Araki Y. Elevation of the soluble form GPI-80, a β2 integrin-associated glycosylphosphatidylinositol anchored protein, in the serum of patients with Wegener’s granulomatosis.
    Allergol.Int. 54:299-303, 2005.
  3. Anzai-Takeda Y., Takeda T., Sendo F., Araki Y.
    Inhibition of cell spreading and stress fiber formation in CHO cells transfected with cDNA of a glycosylphosphatidylinositol-anchored glycoprotein, GPI-80.
    Immunobiology 210:1-10, 2005.
  4. Sendo D., Takeda Y., Watanabe T., Sendo F., Araki Y.
    A monoclonal antibody to GPI-80, a β2 integrin-associated glycosylphosphatidylinositol anchored protein, specifically enhances macropinocytosis in human monocyte.
    Yamagata Med.J. 23:69-82, 2005.
  5. Takayama T., Mishima T., Mori M., Ishikawa T., Takizawa T., Goto T., Suzuki M., Araki Y., Matsubara S., Takizawa T.
    TEX101 is shed from the cell surface of sperm located in the caput epididymidis of the mouse.
    Zygote 13:325-333, 2005.
  6. Fu JF., Takeda Y., Sendo F., Araki Y.
    Different impacts of DMSO and RA on GPI-80 expression.
    Acta Biologiae Experimentalis Sinica 38:37-44, 2005.
  7. Suzuki K., Yu X., Chaurand P., Araki Y., Lareyre JJ., Caprioli RM., Matusik RJ., Orgebin-Crist MC.
    Epididymis-specific promoter-driven gene targeting: A transcription factor which regulates epididymis-specific gene expression.
    Mol.Cell.Endocrinol. 250:184-189, 2006.
  8. Tsukamoto H., Yoshitake H., Mori M., Yanagida M., Takamori K., Ogawa H., Takizawa T., Araki Y.
    Testicular proteins associated with the germ cell-specific protein, TEX101: Involvement of cellubrevin in TEX101-trafficking to the germ cell surface during spermatogenesis.
    Biochem.Biophys.Res.Commun. 345:229-238, 2006.
  9. Jin H., Yoshitake H., Tsukamoto H., Takahashi M., Mori M., Takizawa T., Takamori K., Ogawa H., Kinoshita K., Araki Y.
    Molecular characterization of a germ cell-specific antigen, TEX101, from mouse testis. Zygote 14:201-208, 2006.
  10. Tsukamoto H., Takizawa T., Takamori K., Ogawa H., Araki Y.
    Genomic organization and structure of the 5'-flanking region of the TEX101 gene: Alternative promoter usage and splicing generate transcript variants with distinct 5'-untranslated region.
    Mol.Reprod.Dev. 74:154-162, 2007.
  11. Yoshitake H., Takahashi M., Ishikawa H., Nojima M., Iwanari H., Watanabe A., Aburatani H., Yoshida K., Ishi K., Takamori K., Ogawa H., Hamakubo T., Kodama T., Araki Y.
    Aldo-keto reductase family 1, member B10 in uterine carcinomas: a potential risk factor of recurrence after surgical therapy in cervical cancer.
    Int.J.Gynecol.Cancer 17: 1300-1306, 2007.
  12. Nitto T., Takeda Y., Yoshitake H., Sendo F., Araki Y.
    Structural divergence of GPI-80 in activated neutrophils.
    Biochem.Biophys.Res.Commun. 359:227-233, 2007.
  13. Suzuki K., Yu X., Chaurand P., Araki Y., Lareyre JJ., Caprioli RM., Orgebin-Crist MC., Matusik RJ.
    Epididymis-specific lipocalin promoters.
    Asian J.Androl. 9:515-521, 2007.
  14. Yoshitake H., Shirai Y., Mochizuki Y., Iwanari H., Tsubamoto H.,
    Koyama K., Takamori K., Ogawa H., Hasegawa A., Kodama T., Hamakubo T., Araki Y. Molecular diversity of TEX101, a marker glycoprotein for germ cells monitored with monoclonal antibodies: Variety of the molecular characteristics according to its subcellular localization within the mouse testis. J.Reprod.Immunol. 79:1-11, 2008.
  15. Yoshitake H., Tsukamoto H., Maruyama-Fukushima M, Takamori K., Ogawa H., Araki Y.
    TEX101, a germ cell marker glycoprotein is associated with lymphocyte antigen 6 complex locus k within the mouse testis.
    Biochem.Biophys.Res.Commun. 372:277-282, 2008.
  16. Wakabayashi I., Araki Y.
    Associations of alcohol drinking with blood pressure and serum lipids in female smokers and nonsmokers.
    Gender Med. 6:290-299, 2009.
  17. Shirai Y., Yoshitake H., Maruyama M., Takamori K., Ogawa H., Hasagawa A., Araki Y.
    Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process
    J.Reprod.Dev. 55:240-246, 2009.
  18. Fujiwara H., Araki Y., Toshimori K.
    Is the zona pellucida an intrinsic source of signals activating maternal recognition of the developing mammalian embryo?
    J.Reprod.Immunol. 81:1-8, 2009.
  19. Yamatoya K., Yoshida K., Ito C., Maekawa M., Yanagida M., Takamori K., Ogawa H., Araki Y., Miyado K., Toyama Y., Toshimori K.
    Equatorin: An acrosomal N,O-sialoglycoprotein involved in sperm-egg fusion
    Biol.Reprod. 81:889-897, 2009.
  20. Fujiwara H., Ideta A., Araki Y., Takao Y., Sato Y., Tsunoda N., Aoyagi Y., Konishi I.
    Immune system cooperatively supports endocrine system-primed embryo implantation.
    J.Mammal.Ova.Res. 26:122-128, 2009.
  21. Wakabayashi I., Araki Y.
    Influences of gender and age on relationships between alcohol drinking and atherosclerotic risk factors.
    Alcohol.Clin.Exp.Res. 34:S54-60, 2010.
  22. Fujiwara H., Ideta A., Araki Y., Takao Y., Sato Y., Horie A., Tsunoda N., Aoyagi Y., Konishi I.
    Possible contribution of circulating blood cells to embryo implantation
    Indian J.Physiol.Pharmacol. 54, Suppl 1:51-60, 2010.
  23. Lei Z., Lin J., Li X., Li S., Zhou H, Araki Y., Lan Z-J.
    Postnatal male germ cell expression of the Cre recombinase in Tex101-iCre
    transgenic mice. Genesis 48: 707-722, 2010.
  24. Honda M., Asai T., Umemoto T., Araki Y., Oku N., Tanaka M.
    Supressioion of choroidal neovascularization by single intravitreal injection of APRPG-modified liposomes encapsulating SU5416.
    Arch Ophthalmol 129: 317-321, 2011.
  25. Araki Y., Nonaka D., Tajima A., Maruyama M., Nitto T., Ishikawa H., Yoshitake H., Yoshida E., Kuronaka N., Asada K., Yanagida M., Nojima M., Yoshida K., Takamori K., Hashiguchi T., Maruyama I., Lee L-J., Tanaka K.
    Peptide fragments profiles in the peripheral blood of patients with pregnancy-induced hypertension: Quantitative peptidome analysis by a newly developed technology and the potential utility for early diagnosis.
    Proteomics 2011, in press (DOI: 10.1002/pmic.201000753)
  26. Yoshitake H., Yanagida M., Maruyama M., Takamori K., Hasegawa A., Araki Y.
    Molecular characterization and expression of dipeptidase 3, a testis-specific membrane bound dipeptidase related to an anti-sperm auto-monoclonal antibody epitope. J.Reprod.Immunol. 2011, in press.
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