学校法人順天堂　研究者情報データベース:::研究業績詳細:::

HOME　＞　研究業績詳細

研究業績詳細

高井敏朗（タカイトシロウ）

研究テーマ	アレルギー・自然免疫・獲得免疫・Th分化・バリア・環境-遺伝子相互作用
研究業績（論文）	（2020年6月1日更新） 1. Kamijo S, Hara M, Suzuki M, Nakae S, Ogawa H, Okumura K, Takai T. Innate IL-17A enhances IL-33-independent skin eosinophilia and IgE response on subcutaneous papain sensitization. J Invest Dermatol.(2020, In-press) 2. 高井敏朗, 上條清嗣. アレルゲンと自然免疫. 臨床免疫アレルギー科, 73:160-167 (2020) [Review] 3. Maruyama N, Takai T, Kamijo S, Suchiva P, Takeshige T, Suzuki M, Hara M, Matsuno K, Harada S, Narada N, Nakae S, Sudo K, Okuno T, Yokomizo T, Ogawa H, Okumura K, Ikeda S. Cyclooxygenase inhibition in mice hightens adaptive-and innate-type responses against inhaled protease allergen and IL-33. Allergy, 74: 2237-2240 (2019) 4. 高井敏朗.　アレルゲンのアジュバント作用. 日本職業・環境アレルギー学会雑誌 26:1-8 (2019) [Review] 5. 高井敏朗.　2 病態の新しい考え方　7) ダニの関与．皮膚科の臨床 (増刊　変わりつつあるアトピー性皮膚炎の常識-最新の知識と治療の極意-) 61:759-764 (2019) [Review] 6. Nishioka I, Takai T, Maruyama N, Kamijo S, Suchiva P, Suzuki M, Kunimine S, Ochi H, Shimura S, Sudo K, Ogawa H, Okumura K, Ikeda S. Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation. J Allergy Clin Immunol 141:2271-2273.e7 (2018) 7. 高井敏朗、清村咲子、飯田秀雄、丸山奈津子、越智啓乃、上條清嗣、西岡いずみ、原むつ子、松田彰、斎藤博久、中江進、小川秀興、奧村康、池田志斈. アレルゲンのプロテアーゼ活性と機械的な皮膚バリア損傷はマウスにおいて経皮的アレルゲン感作を増強する. 臨床免疫・アレルギー科68:515-522 (2017) [Review] 8. 高井敏朗. ダニ抗原について. 最新醫學別冊　診断と治療ABC127「アレルギー性鼻炎」 pp128-19 (2017) [Review] 9. 高井敏朗. 免疫学・アレルギー学の基礎：アレルゲンと感作・発症の機序. 第51会日本アレルギー学会専門医教育セミナーテキストpp1-6. (2017) [講習会テキスト] 10. Ochi H, Takai T, Shimura S, Maruyama N, Nishioka I, Kamijo S, Iida H, Nakae S, Ogawa H, Okumura K, Ikeda S. Skin treatment with detergent promotes protease allergen-dependent epicutaneous sensitization in a manner different from tape stripping in mice. J Invest Dermatol 137:1578-1582 (2017) 11. 高井敏朗, 上條清嗣, 布村聡, 羅智靖, 小川秀興, 奧村康. 好塩基球の自然免疫応答によるIL-4産生のFc受容体γ鎖依存性　臨床免疫・アレルギー科 66:371-374 (2016) [Review] 12. 高井敏朗. 環境アレルゲン. 日本医師会雑誌特別号「アレルギー疾患の全て」145:S55-S56 (2016) [Review] 13. 高井敏朗. 専門医のためのアレルギー講座：アレルゲンとその修飾因子. アレルギー 65:159-165 (2016) [Review] 14. 高井敏朗. 家塵ダニアレルゲンの性状と標準化. 喘息・アレルギー 29:15-19 (2016) [Review] 15. Shimura S, Takai T, Iida H, Maruyama N, Ochi H, Kamijo S, Nishioka I, Hara M, Matsuda A, Saito H, Nakae S, Ogawa H, Okumura K, Ikeda S. Epicutaneous allergic sensitization by cooperation between allergen protease-activity and mechanical skin barrier damage in mice. J Invest Dermatol 136:1408-17 (2016) 16. Kamijo S, Suzuki M, Hara M, Shimura S, Ochi H, Maruyama N, Matsuda A, Saito H, Nakae S, Suto H, Ichikawa S, Ikeda S, Ogawa H, Okumura K, Takai T. Subcutaneous allergic sensitization to protease allergen is dependent on mast cells but not IL-33: Distinct mechanisms between subcutaneous and intranasal routes. J Immunol 196:3559-69 (2016) 17. Kamijo S, Nunomura S, Ra C, Kanaguchi Y, Suzuki Y, Ogawa H, Okumura K, Takai T. Innate basophil IL-4 responses against allergens, endotoxin, and cytokines require the Fc receptor gamma-chain. J Allergy Clin Immunol 137:1613-1615.e2 (2016) 18. Suzuki M, Hara M, Ichikawa S, Kamijo S, Nakazawa T, Hatanaka H, Akiyama K, Ogawa H, Okumura K, Takai T. Presensitization to Ascaris antigens promotes induction of mite-specific IgE upon mite antigen inhalation in mice. Allergol Int 65:44-51 (2016) 19. 高井敏朗：IL-33とアレルゲン.　医学のあゆみ :252:1226-1232 (2015) [Review] 20. Takai T. Immunotherapy to treat allergies: Recent advances and future prospects. Juntendo Med J 61:597-600 (2015) [Review] 21. Takai T, Izuhara K. Allergens in modern society: Updated catalogs and future prospects. Allergol Int 64:293-294 (2015) [Editorial] 22. Pichler U, Hauser M, Wolf M, Bernardi ML, Gadermaier G, Weiss R, Ebner C, Yokoi H, Takai T, Didierlaurent A, Rafaiani C, Briza P, Mari A, Behrendt H, Wallner M, Ferreira F. Pectate lyase pollen allergens: Sensitization profiles and cross-reactivity pattern PLoS One. 10:e0120038 (2015) 23. Takai T, Okamoto Y, Okubo K, Nagata M, Sakaguchi M, Fukutomi Y, Saito A, Yasueda H, Masuyama K. Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines–secondary publication. Allergol Int 64:181-186 (2015) 24. Nishikado H, Fujimura T, Taka H, Mineki R, Ogawa H, Okumura K, Takai T, Cysteine protease antigens cleave CD123, the  subunit of murine IL-3 receptor, on basophils and suppress IL-3-mediated basophil expansion. Biochem Biophys Res Commun, 460(2):261-6 (2015) 25. 高井敏朗. 環境アレルゲン解析の進歩. 小児科診療77:1241-1247 (2014) 26. 高井敏朗, 岡本美孝, 大久保公裕, 永田真, 阪口雅弘, 福冨友馬, 齋藤明美, 安枝浩, 増山敬祐. ダニアレルゲンワクチン標準化に関する日本アレルギー学会タスクフォース報告. アレルギー 63:1229-1239 (2014) 27. Zhang C, Ohno T, Kang S, Takai T, Azuma M. Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets. Vaccine. 32:5669-76 (2014) 28. Iida H, Takai T, Hirasawa Y, Kamijo S, Shimura S, Ochi H, Nishioka I, Maruyama N, Ogawa H, Okumura K, Ikeda S. Epicutaneous administration of papain induces IgE and IgG responses in a cysteine protease activity-dependent manner. Allergol Int 63:319-226 (2014) 29. Takai T, Chen X, Xie Y, Vu AT, Le TA, Kinoshita H, Kawasaki J, Kamijo S, Hara M, Ushio H, Baba T, Hiramatsu K, Ikeda S, Ogawa H, Okumura K. TSLP expression induced via Toll-like receptor pathways in human keratinocytes. Methods Enzymol 535:371-387 (2014) 30. Kamijo S, Takeda H, Tokura T, Suzuki M, Inui K, Hara M, Matsuda H, Matsuda A, Oboki K, Ohno T, Saito H, Nakae S, Sudo K, Suto H, Ichikawa S, Ogawa H, Okumura K, Takai T. IL-33-mediated innate response and adaptive immune cells contribute to maximum responses of protease allergen-induced allergic airway inflammation. J Immunol 190:4489-99 (2013) 31. Chen X, Takai T, Xie Y, François Niyonsaba. Okumura K. Ogawa H. Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes. Biochem Biophys Res Commun 433:532-7 (2013) 32. Nakazawa T, Khan AF, Yasueda H, Saito A, Fukutomi Y, Takai T, Zaman K, Yunus M, Takeuchi H, Iwata T, Akiyama K: Immunization of rabbits with nematode Ascaris lumbricoides antigens induces antibodies cross-reactive to house dust mite Dermatophagoides farinae antigens. Biosc Biotechnol Biochem 77:145-150 (2013) 33. Takai T, Mite endopeptidase 1. In: Neil D. Rawlings and Guy S. Salvesen, editors, Handbook of Proteolytic Enzymes. Oxford: Academic Press, 2013, pp.1957-1963. 34. Takai T, Serine endopeptidase allergens from Dermatophagoides species. In: Neil D. Rawlings and Guy S. Salvesen, editors, Handbook of Proteolytic Enzymes. Oxford: Academic Press, 2013, pp.3055-3060. 35. 高井敏朗：アレルゲンはなぜアレルゲンになるのか？：アレルゲンが引き起こすバリア破壊と自然免疫応答.　実験医学 31: 2713-2719 (2013) 36. 高井敏朗：ダニアレルゲンによる経皮感作のメカニズム. アレルギー・免疫 20:854-860 (2013) 37. Xie Y, Takai T, Chen X, Okumura K, Ogawa H: Long TSLP transcript expression and release of TSLP induced by TLR ligands and cytokines in human keratinocytes. 66:233-237 (2012) 38. Saeki M, Nishimura T, Kaminuma O, Suzuki K, Takai T, Mori A, Takada K, Takaiwa F, Hiroi T. Inhibition of allergen-induced airway inflammation by low-dose oral immunotherapy with transgenic rice seeds independently of immunoglobulin E synthesis. Int Arch Allergy Immunol 158:66-69 (2012) 39. Takai T. TSLP expression: cellular sources, triggers, and regulatory mechanisms. Allergol Int 61:3-17 (2012) 40. 高井敏朗：アレルゲンの３つの顔. アレルギー61:930-940 (2012) 41. 高井敏朗：私は思う：アレルゲンとは何か．アレルギーの臨床 32:739-741 (2012) 42. 高井敏朗、池田志斈：ダニアレルギーとアレルゲン週刊日本医事新報 4582号57-59（2012） 43. 高井敏朗：Th2アジュバントとしての環境アレルゲンと黄色ブドウ菌：経皮的Th2感作におけるバリア破壊と自然免疫応答日本皮膚アレルギー・接触皮膚炎学会雑誌 6:8-13 (2012) 44. 高井敏朗：アレルゲンの持つアジュバント作用と抗原感作機構アレルギー・免疫 19:12-19（2012） 45. Vu AT, Chen X, Xie Y, Kamijo S, Ushio H, Kawasaki J, Hara M, Ikeda S, Okumura K, Ogawa H, Takai T. Extracellular double-stranded RNA induces TSLP via an endosomal acidification- and NF-kB-dependent pathway in human keratinocytes. J Invest Dermatol 131:2205-2212 (2011) 46. Kawasaki J, Ushio H, Kinoshita H, Fukai T, Niyonsaba F, Takai T, Ogawa H, Okumura K, Ikeda S: Viral infection induces Thymic stromal lymphopoietin (TSLP) in human keratinocyte. J Dermatol Sci 62:131-134 (2011) 47. Takai T, Ikeda S. Barrier dysfunction caused by environmental proteases in the pathogenesis of allergic diseases. Allergol Int 60:25-35 (2011) 48. Le TA, Takai, T, Vu AT, Kinoshita H, Chen X, Ikeda S, Ogawa H, Okumura K. Flagellin induces the expression of thymic stromal lymphopoietin in human keratinocytes via Toll-like receptor 5. Int Arch Allergy Immunol 155:31-37 (2011) 49. Takai T. Missions of protease allergens in the epithelium. Int Arch Allergy Immunol 154:3-5 (2011) 50. Suzuki K, Kaminuma O, Yang L, Takai T, Mori A, Umezu-Goto M, Ohtomo T, Ohmachi Y, Noda Y, Hirose S, Okumura K, Ogawa H, Takada K, Hirasawa M, Hiroi T, Takaiwa F. Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression. Plant Biotechnol J 9:982-990 (2011) 51. 高井敏朗：アレルゲンはなぜアレルゲンになるのか　アレルギー 60:809-816 (2011) 52. 高井敏朗：表皮ケラチノサイトにおけるTSLP産生誘導　臨床免疫・アレルギー科 55:365-370 (2011) 53. Vu AT, Baba T, Chen X, Le TA, Kinoshita H, Xie Y, Kamijo S, Hiramatsu K, Ikeda S, Ogawa H, Okumura K, Takai T. Staphylococcus aureus membrane and diacylated lipopeptide induce thymic stromal lymphopoietin in keratinocytes via the Toll-like receptor 2-Toll-like receptor 6 pathway. J Allergy Clin Immunol 126:985-993.e3 (2010) 54. Hirasawa Y, Takai T, Nakamura T, Mitsuishi K, Gunawan H, Suto H, Ogawa T, Wang XL, Ikeda S, Okumura K, Ogawa H. Staphylococcus aureus extracellular protease causes epidermal barrier dysfunction. J Invest Dermatol 130:614-617 (2010) 55. Takai T. Cedar allergen harvest from tobacco: Plant biotechnology for recombinant allergens. Int Arch Allergy Immunol 153:431-433 (2010) 56. Le TA, Takai T, Vu AT, Kinoshita H, Ikeda S, Ogawa H, Okumura K, Glucocorticoids inhibit double-stranded RNA-induced thymic stromal lymphopoietin release from keratinocytes in atopic cytokine milieu more effectively than tacrolimus. Int Arch Allergy Immunol 153:27-34 (2010) 57. Vu AT, Takai T, Le TA, Kinoshita H, Kamijo S, Hara M, Wang XL, Ogawa T, Ikeda S, Ogawa H, Okumura K. Proinflammatory response in human keratinocytes stimulated with ligands for TLR2, TLR1, and TLR6. Juntendo Medical Journal 56:50-55 (2010) 58. 高井敏朗：ダニに由来するTh2アジュバント活性　臨床免疫・アレルギー科 54:619-624 (2010) 59. 高井敏朗、上條清嗣：花粉のTh2アジュバント活性　臨床免疫・アレルギー科 54:625-630 (2010) 60. 上條清嗣、奥村康、高井敏朗：樹状細胞の花粉成分によるTh2細胞誘導　臨床免疫・アレルギー科 54: 313-322 (2010) 61. 高井敏朗：アレルゲンに対する自然免疫応答呼吸 29:878-883 (2010) 62. 高井敏朗：知っておかなければならないアレルギー疾患の基礎知識 (2) Th2アジュバントとしてのアレルゲン　アレルギー・免疫 17:687-694 (2010) 63. 上條清嗣、奥村康、高井敏朗：花粉による樹状細胞の活性化　臨床免疫・アレルギー科 53: 312-320 (2010) 64. 奥村康、高井敏朗：環境因子とアレルギー疾患—特にアトピー性皮膚炎を中心にー　日中医学24: 47-52 (2010) 65. Takai T, Kato T, Hatanaka H, Inui K, Nakazawa T, Ichikawa S, Mitsuishi K, Ogawa H, Okumura K. Modulation of allergenicity of major house dust mite allergens Der f 1 and Der p 1 by interaction with an endogenous ligand. J Immunol 183:7958-7965 (2009) 66. Kamijo S, Takai T, Kuhara T, Tokura T, Ushio H, Ota M, Harada N, Ogawa H, Okumura K. Cupressaceae pollen grains modulate dendritic cell response and exhibit IgE-inducing adjuvant activity in vivo. J Immunol 183: 6087-6094 (2009) 67. Kato T, Takai T, Fujimura T, Matsuoka H, Ogawa T, Murayama K, Ishii A, Ikeda S, Okumura K, Ogawa H. Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes Allergy 64:1366-74 (2009) 68. Takai T, Vu AT, Le TA, Kinoshita H, Ushio H, Ikeda S, Ogawa H, Okumura K. Reply. J Allergy Clin Immunol 124:864-5 (2009) 69. Ichikawa S, Takai T, Yashiki T, Takahashi S, Okumura K, Ogawa H, Kohda D, Hatanaka H. Lipopolysaccharide binding of the mite allergen Der f 2. Genes Cells 14:1055-65 (2009). 70. Wang XL, Takai T, Kamijo S, Gunawan H, Ogawa H, Okumura K. NADPH oxidase activity in allergenic pollen grains of different plant species. Biochem Biobhys Res Commun 387:430-434 (2009) 71. Le TA, Takai T, Kinoshita H, Suto H, Ikeda S, Okumura K, Ogawa H. Inhibition of double-stranded RNA-induced TSLP in human keratinocytes by glucocorticoids. Allergy 64:1231-1232 (2009) 72. Suzuki K, Kaminuma O, Yang L, Motoi Y, Takai T, Ichikawa S, Okumura K, Ogawa H, Mori A, Takaiwa F, Hiroi T. Development of transgenic rice expressing mite antigen for a new concept of immunotherapy. Int Arch Allergy Immunol 149 Suppl 1:21-24 (2009) 73. Takai T, Ochiai Y, Ichikawa S, Sato E, Ogawa T, Tokura T, Kuhara T, Kawai H, Hatanaka H, Takakhashi S, Ogawa H, Okumura K. Enzyme-linked immunosorbent assays with high sensitivity for antigen-specific and total murine IgE: Useful tool for study of allergies in mouse models. Allergol Int 58:225-235 (2009). 74. Seto T, Takai T, Ebihara N, Matsuoka H, Wang XL, Ishii A, Ogawa H, Murakami A, Okumura K. SLPI prevents cytokine release in mite protease-exposed conjunctival epithelial cells. Biochem Biobhys Res Commun 379:681-685 (2009) 75. Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Vu AT, Kawasaki J, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol123:179-86 (2009) 76. 高井敏朗：アレルゲン研究の最前線実験医学（増刊号：アレルギー疾患の免疫機構） 27:3340-3347 (2009) 77. 高井敏朗：ダニのアレルゲン活性とプロテアーゼ臨床免疫アレルギー科 52:669-676. 2009 78. 高井敏朗：アレルゲンに関する臨床・基礎の最近のトピックスMinophagen Medical Review 54:71-83 (2009) 79. 高井敏朗：抗原の成り立ち(5)新しい形のアレルゲンワクチン. Q&Aでわかるアレルギー疾患 5(3):338-342 (2009) 80. 高井敏朗：抗原の成り立ち(4)アレルゲンはなぜアレルゲンになるのか？. Q&Aでわかるアレルギー疾患 5(2):218-221 (2009) 81. 高井敏朗：抗原の成り立ち(3)アレルゲン分子のいろいろ. Q&Aでわかるアレルギー疾患 5(1):96-99 (2009) 82. Gunawan H, Takai T, Kamijo S, Wang XL, Ikeda S, Okumura K, Ogawa H: Characterization of proteases, proteins, and eicosanoid-like substances in soluble extracts from allergenic pollen grains Int Arch Allergy Immunol 147:276-288 (2008). 83. Ogawa T, Takai T, Kato T, Kikuchi T, Niyonsaba F, Ikeda S, Okumura K, Ogawa H: Upregulation of release of granulocyte-macrophage colony-stimulating factor from keratinocytes stimulated with cysteine protease activity of recombinant major mite allergens, Der f 1 and Der p 1. Int Arch Allergy Immunol 146:27-35 (2008). 84. Gunawan H, Takai T, Ikeda S, Okumura K, Ogawa H: Protease activity of allergenic pollen of cedar, cypress, juniper, birch, and ragweed. Allergol Int 57:83-91 (2008). 85. 高井敏朗：抗原の成り立ち(2)アレルギー原因物質からアレルゲン分子へ. Q&Aでわかるアレルギー疾患 4(6): 602-605 (2008) 86. 高井敏朗：抗原の成り立ち(1)アレルゲンに対する獲得免疫：IgE抗体とTh2応答. Q&Aでわかるアレルギー疾患 4(5): 526-529 (2008) 87. 高井敏朗：アレルゲンに含有されるプロテアーゼとアレルギー反応の誘導. 臨床免疫アレルギー科50(6):625-631 (2008) 88. 高井敏朗：組換体アレルゲンワクチン. アレルギーの臨床 28(10):865-870 (2008) 89. 高井敏朗：ダニ主要アレルゲンの構造・機能と疾患誘導の関連性の解析およびワクチン設計：リコンビナントアレルゲンの応用．[第１７回アボットジャパン・アレルギー学術奨励賞]アレルギア第36号, 60-64 (2007) 90. Nakamura T, Hirasawa Y, Takai T, Mitsuishi K, Okuda M, Kato T, Okumura K, Ikeda S, Ogawa H.: Reduction of skin barrier function by proteolytic activity of a recombinant house dust mite allergen Der f 1. J Invest Dermatol 126:2719-2723(2006) 91. Kikuchi Y, Takai T, Kuhara T, Ota M, Kato T, Hatanaka H, Ichikawa S, Tokura T, Akiba H, Mitsuishi K, Ikeda S, Okumura K, Ogawa H: Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p 1 to sensitization towards IgE and IgG responses. J Immunol 177:1609-1617 (2006). 92. Kikuchi Y, Takai T, Ota M, Kato T, Takeda K, Mitsuishi K, Ikeda S, Okumura K, Ogawa H: Application of immunoreaction enhancer solutions to an enzyme-linked immunosorbent assay for antigen-specific IgE in mice immunized with recombinant major mite allergens or ovalbumin. Int Arch Allergy Immunol 141:322-330 (2006). 93. Takai T, Mizuuchi E, Kikuchi Y, Nagamune T, Okumura K, Ogawa H: Glycosylation of recombinant proforms of major house dust mite allergens Der p 1 and Der f 1 decelerates the speed of maturation Int Arch Allergy Immunol 139:181-187 (2006). 94. 高井敏朗：プロテアーゼとしてのダニ抗原の意義．先端医療シリーズ３８皮膚疾患の最新医療（斎田俊明、飯塚一編）、先端医療技術研究所、東京、p90-94 (2006) 95. 高井敏朗：ダニアレルゲン研究の現状. 臨床免疫・アレルギー科 46: 619-625 (2006) 96. 高井敏朗：ダニグループ１アレルゲン組換体の作製とその活性．アレルギー・免疫 19: 332-339 (2006) 97. Kato T, Takai T, Mitsuishi K, Okumura K, Ogawa H: Cystatin A inhibits IL-8 production by keratinocytes stimulated with Der p 1 and Der f 1: Biochemical skin-barrier against mite cysteine proteases. J Allergy Clin Immunol, 116:169-76 (2005). 98. Takai T, Kato T, Ota M, Yasueda H, Kuhara T, Okumura K, Ogawa H: Recombinant Der p 1 and Der f 1 with in vitro enzymatic activity to cleave human CD23, CD25, and alpha1-antitrypsin, and in vivo IgE-eliciting activity in mice. Int Arch Allergy Immunol, 137:194-200 (2005). 99. Takai T, Kato T, Yasueda H, Okumura K, Ogawa H. Analysis of the structure and allergenicity of recombinant pro- and mature Der p 1 and Der f 1: Major conformational IgE-epitopes blocked by prodomains. J Allergy Clin Immunol 115:555-563 (2005). 100. Nakazawa T, Takai T, Hatanaka H, Mizuuchi E, Nagamune T, Okumura K, Ogawa H: Multiple-mutation at a potential ligand-binding region decreased allergenicity of a mite allergen Der f 2 without disrupting global structure. FEBS Lett 579:1988-1994 (2005). 101. Takai T, Kato T, Sakata Y, Yasueda H, Izuhara K, Okumura K, Ogawa H: Recombinant Der p 1 and Der f 1 exhibit cysteine protease activity but no serine protease activity. Biochem Biophys Res Commun 328:944-52 (2005). 102. Takai T, Takaoka M, Yasueda H, Okumura K, Ogawa H: Dilution-method to refold bacterially expressed recombinant Der f 2 and Der p 2 to exhibit the secondary structure and histamine-releasing activity of natural allergens. Int Arch Allergy Immunol 137:1-8 (2005). 103. Ichikawa S, Takai T, Inoue T, Yuuki T, Okumura Y, Ogura K, Inagaki F, Hatanaka H: NMR study on the major mite allergen Der f 2: Its refined tertiary structure, epitopes for monoclonal antibodies and characteristics shared by ML protein group members. J Biochem 137:255-263 (2005). 104. 高井敏朗：IgE産生とイエチリダニ由来プロテアーゼ．臨床免疫 44:541-545 (2005) 105. 高井敏朗：ダニ主要アレルゲンの構造と機能．臨床検査 49:739-747 (2005) 106. Sakata Y, Arima K, Takeshita K, Takai T, Aoki S, Ogawa H, Sugihara H, Fujimoto K, Izuhara K. Characterization of novel squamous cell carcinoma antigen-related molecules in mice. Biochem Biophys Res Commun 324:1340-1345 (2004). 107. Sakata Y, Arima K, Takai T, Sakurai W, Masumoto K, Yuyama N, Suminami Y, Kishi F, Yamashita T, Kato T, Ogawa H, Fujimoto K, Matsuo Y, Sugita Y, Izuhara K. The squamous cell carcinoma antigen 2 inhibits the cysteine proteinase activity of a major mite allergen, Der p 1. J Biol Chem. 279:5081-7 (2004). 108. 高井敏朗: アレルゲンの構造と機能：アレルギー発症との関連と、治療への新しいアプローチ. 喘息 17: 15-20 (2004) 109. Roeber D, Achari A, Takai T, Okumura Y, Scott DL. Crystallization and preliminary X-ray analysis of Der f 2, a potent allergen derived from the house dust mite (Dermatophagoides farinae). Acta Cryst D59:1046-1048 (2003). 110. Takai T, Mineki R, Nakazawa T, Takaoka M, Yasueda H, Murayama K, Okumura K, Ogawa H. Maturation of recombinant mite allergen Der p 1 and Der f 1, and its implication in the blockade of proteplytic activity. FEBS Lett 531:265-272 (2002) 111. 高井敏朗: アレルゲンエンジニアリング —アレルゲンの構造・機能とアレルギー・ネットワークの理解を基盤とした次世代の治療戦略—. 臨床免疫 38:298-305 (2002) 112. 高井敏朗: ダニ抗原のエンジニアリング—. アレルギー科 14:285-291 113. 羅智靖、高橋恭子、西山千春、高井敏朗、柳原行義　高親和性IgEレセプター(FceRI)を標的としたマスト細胞活性化制御の展望アレルギー科14,73-82 (2002) 114. Yasuhara T, Takai T, Yuuki T, Okudaira H, Okumura Y. Biologically active recombinant forms of a major house dust mite group 1 allergen Der f 1 with full activities of both cysteine protease and IgE binding. Clin Exp Allergy 31:116-124 (2001). 115. Takahashi K, Takai T, Yasuhara T, Yokota T, Okumura Y. Effects of site-directed mutagenesis in the cysteine residues and the N-glycosylation motif in recombinant Der f 1 on secretion and protease activity. Int Arch Allergy Immunol 124:454-460 (2001). 116. Takai T, Okumura K, Ra C, Yokota T, Okumura Y. Expression of humanized Fab fragments that recognize the IgE-binding domain of human FcεRI α chain. J Biochem 129:5-12 (2001). 117. Takai T, Hatanaka H, Ichikawa S, Yokota T, Inagaki F, Okumura Y. Effects of double-mutation at two distant IgE-binding sites in the three dimensional structure of the major house dust mite allergen Der f 2 on IgE-binding and histamine releasing activity. Biosc Biotechnol Biochem 65:1601-1609 (2001). 118. Takai T, Akagawa-Chihara M, Yokota T, Okumura Y. 2001. Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting. Biosc Biotechnol Biochem 65:694-697 (2001). 119. Yasuhara T, Takai T, Yuuki T, Okudaira H, Okumura Y. 2001. Cloning and expression of cDNA encoding the complete prepro-form of an isoform of Der f 1, the major group 1 allergen from house dust mite Dermatophagoides farinae. Biosc Biotechnol Biochem 65:563-569 (2001). 120. Takai T, Takahashi K, Akagawa-Chihara M, Fukada M, Yuuki T, Shibuya I, Okumura K, Ra C, Yokota T, Okumura Y. Production of humanized antibody against human high-affinity IgE receptor in a serum free culture of CHO cells and purification of the Fab fragments. Biosc Biotechnol Biochem 65:1082-1089 (2001). 121. Takai T, Okumura K, Ra C. Direct expression of the extracellular portion of human FcεRI α chain in Escherichia coli. Biosc Biotechnol Biochem 65:79-85 (2001). 122. Takai T, Ichikawa S, Yokota T, Hatanaka H, Inagaki F, Okumura Y. Unlocking the allergenic structure of the major house dust mite allergen Der f 2 by elimination of key intramolecular interactions. FEBS Letters 482:102-107 (2000) 123. Takai T, Ichikawa S, Hatanaka H, Inagaki F, Okumura Y. Effects of proline mutations in the major house dust mite allergen Der f 2 on IgE-binding and histamine-releasing activity. European Journal of Biochemistry 267:6650-6656 (2000) 124. Takai T, Yuuki T, Ra C. Inhibition of IgE-dependent histamine release from human peripheral blood basophils by humanized Fab fragments that recognize the membrane proximal domain of the human FcεRI α chain. International Archives of Allergy and Immunology 123:308-318 (2000) 125. Takahashi K, Takai T, Yasuhara T, Yuuki T, Ohtake Y, Yokota T, Okumura Y. Production of enzymatically and immunologically active Der f 1 in Escherichia coli. International Archives of Allergy and Immunology 122:108-114 (2000) 126. Takahashi K, Yuuki T, Takai T, Ra C, Yokota T, Okumura Y. Production of humanized Fab fragment against human high affinity IgE receptor in Pichia pastoris. Bioscience, Biotechnology, Biochemistry 64:2138-2144 (2000) 127. Takai T, Yuuki T, Iwamoto-Yasue N, Okumura K, Ra C. 2000. Epitope analysis and primary structures of variable regions of anti-human FcεRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Bioscience, Biotechnology, and Biochemistry 64:1856-1867 (2000) 128. 高井敏朗: 減感作療法への応用を目的とした“アレルゲン・エンジニアリング”—遺伝子工学的に改変したダニ主要アレルゲンDer f 2におけるIgEエピトープの破壊とT細胞エピトープの温存—．アレルギー科10:173-178 (2000) 129. 高井敏朗: アレルゲンエンジニアリング”—ダニ主要アレルゲンDer f 2の改変—．医学のあゆみ192:983-988 (2000) 130. Takai T, Mori A, Yuuki T, Okudaira H, Okumura Y. Non-anaphylactic combination of partially deleted fragments of the major house dust mite allergen Der f 2 for allergen-specific immunotherapy. Molecular Immunology 36:1055-1065 (1999) 131. Yasue M, Yokota T, Fukada M, Takai T, Suko M, Okudaira H, Okumura Y. 1998. Hyposensitization to allergic reaction in rDer f 2-sensitized mice by the intranasal administration of a mutant of rDer f 2, C8/119S. Clinical and Experimental Immunology 113:1-9 (1998) 132. 安江正明、高井敏朗、中村伸、羅智靖．ヒト型抗FcεRI抗体によるアレルギーの制御．アレルギー科6:388-394 (1998) 133. 高井敏朗、羅智靖: 抗IgE, 抗IgE受容体療法．現代医療 30:847-852 (1998) 134. Takai T, Yokota T, Yasue M, Nishiyama C, Yuuki T, Mori A, Okudaira H, Okumura Y. 1997. Engineering of the major house dust mite allergen Der f 2 for allergen-specific immunotherapy. Nature Biotechnology 15:754-758. 135. Takai T, Yuuki T, Okumura Y, Mori A, Okudaira H. 1997. Determination of the N- and C-terminal sequences required to bind human IgE of the major house dust mite allergen Der f 2 and epitope mapping for monoclonal antibodies. Molecular Immunology 34:255-261. 136. Takai T, Yuuki T, Okumura K, Ra C. 1997. An approach to control allergic reaction by blocking the binding of IgE to the high-affinity IgE receptor (FcεRI): Fab fragments of humanized anti-human FcεRIα antibody prevent degranulation of human basophils. Japanese Journal of Inflammation 17:557-562. 137. 羅智靖、高井敏朗．1997. IgE-FcεRI-マスト細胞枢軸を標的にしたアレルギーの新しい治療戦略．モレキュラーメディシン34:1534-1541 (1997) 138. 高井敏朗、羅智靖．ヒト型化抗体．アレルギー科4:478-484 (1997) 139. 高井敏朗、羅智靖．IgE結合阻害薬．内科 76:760-766 (1995) 140. 高井敏朗、羅智靖．高親和性IgE受容体．小児科臨床 57(Suppl.):27-32 (1994) 141. Nishiyama C, Yuuki T, Takai T, Okumura Y, Okudaira H. 1993. Determination of three disulfide bonds in a major house dust mite allergen, Der f II. International Archives of Allergy and Immunology 101:159-166. 142. 奥平博一、須甲松伸、結城敏文、高井敏朗、岩本奈美子、奥村康、安藤徹、猪好孝、山川洋志、岩城正廣．ダニアレルゲンの分子遺伝学的解析．Medical Immunology 23:125-133 (1992) 143. Nakano H, Takai T, Kawakami Y, Nishimura H, Iwakura M 1991. Secretion of a cytoplasmic protein using Escherichia coli α-hemolysin membrane transport system. Kagaku Kogaku Ronbunshu 17:687-693.

研究テーマ

アレルギー・自然免疫・獲得免疫・Th分化・バリア・環境-遺伝子相互作用

研究業績（論文）

（2020年6月1日更新）
1. Kamijo S, Hara M, Suzuki M, Nakae S, Ogawa H, Okumura K, Takai T. Innate IL-17A enhances IL-33-independent skin eosinophilia and IgE response on subcutaneous papain sensitization. J Invest Dermatol.(2020, In-press)
2. 高井敏朗, 上條清嗣. アレルゲンと自然免疫. 臨床免疫アレルギー科, 73:160-167 (2020) [Review]
3. Maruyama N, Takai T, Kamijo S, Suchiva P, Takeshige T, Suzuki M, Hara M, Matsuno K, Harada S, Narada N, Nakae S, Sudo K, Okuno T, Yokomizo T, Ogawa H, Okumura K, Ikeda S. Cyclooxygenase inhibition in mice hightens adaptive-and innate-type responses against inhaled protease allergen and IL-33. Allergy, 74: 2237-2240 (2019)
4. 高井敏朗.　アレルゲンのアジュバント作用. 日本職業・環境アレルギー学会雑誌 26:1-8 (2019) [Review]
5. 高井敏朗.　2 病態の新しい考え方　7) ダニの関与．皮膚科の臨床 (増刊　変わりつつあるアトピー性皮膚炎の常識-最新の知識と治療の極意-) 61:759-764 (2019) [Review]
6. Nishioka I, Takai T, Maruyama N, Kamijo S, Suchiva P, Suzuki M, Kunimine S, Ochi H, Shimura S, Sudo K, Ogawa H, Okumura K, Ikeda S. Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation. J Allergy Clin Immunol 141:2271-2273.e7 (2018)
7. 高井敏朗、清村咲子、飯田秀雄、丸山奈津子、越智啓乃、上條清嗣、西岡いずみ、原むつ子、松田彰、斎藤博久、中江進、小川秀興、奧村康、池田志斈. アレルゲンのプロテアーゼ活性と機械的な皮膚バリア損傷はマウスにおいて経皮的アレルゲン感作を増強する. 臨床免疫・アレルギー科68:515-522 (2017) [Review]
8. 高井敏朗. ダニ抗原について. 最新醫學別冊　診断と治療ABC127「アレルギー性鼻炎」 pp128-19 (2017) [Review]
9. 高井敏朗. 免疫学・アレルギー学の基礎：アレルゲンと感作・発症の機序. 第51会日本アレルギー学会専門医教育セミナーテキストpp1-6. (2017) [講習会テキスト]
10. Ochi H, Takai T, Shimura S, Maruyama N, Nishioka I, Kamijo S, Iida H, Nakae S, Ogawa H, Okumura K, Ikeda S. Skin treatment with detergent promotes protease allergen-dependent epicutaneous sensitization in a manner different from tape stripping in mice. J Invest Dermatol 137:1578-1582 (2017)
11. 高井敏朗, 上條清嗣, 布村聡, 羅智靖, 小川秀興, 奧村康. 好塩基球の自然免疫応答によるIL-4産生のFc受容体γ鎖依存性　臨床免疫・アレルギー科 66:371-374 (2016) [Review]
12. 高井敏朗. 環境アレルゲン. 日本医師会雑誌特別号「アレルギー疾患の全て」145:S55-S56 (2016) [Review]
13. 高井敏朗. 専門医のためのアレルギー講座：アレルゲンとその修飾因子. アレルギー 65:159-165 (2016) [Review]
14. 高井敏朗. 家塵ダニアレルゲンの性状と標準化. 喘息・アレルギー 29:15-19 (2016) [Review]
15. Shimura S, Takai T, Iida H, Maruyama N, Ochi H, Kamijo S, Nishioka I, Hara M, Matsuda A, Saito H, Nakae S, Ogawa H, Okumura K, Ikeda S. Epicutaneous allergic sensitization by cooperation between allergen protease-activity and mechanical skin barrier damage in mice. J Invest Dermatol 136:1408-17 (2016)
16. Kamijo S, Suzuki M, Hara M, Shimura S, Ochi H, Maruyama N, Matsuda A, Saito H, Nakae S, Suto H, Ichikawa S, Ikeda S, Ogawa H, Okumura K, Takai T. Subcutaneous allergic sensitization to protease allergen is dependent on mast cells but not IL-33: Distinct mechanisms between subcutaneous and intranasal routes. J Immunol 196:3559-69 (2016)
17. Kamijo S, Nunomura S, Ra C, Kanaguchi Y, Suzuki Y, Ogawa H, Okumura K, Takai T. Innate basophil IL-4 responses against allergens, endotoxin, and cytokines require the Fc receptor gamma-chain. J Allergy Clin Immunol 137:1613-1615.e2 (2016)
18. Suzuki M, Hara M, Ichikawa S, Kamijo S, Nakazawa T, Hatanaka H, Akiyama K, Ogawa H, Okumura K, Takai T. Presensitization to Ascaris antigens promotes induction of mite-specific IgE upon mite antigen inhalation in mice. Allergol Int 65:44-51 (2016)
19. 高井敏朗：IL-33とアレルゲン.　医学のあゆみ :252:1226-1232 (2015) [Review]
20. Takai T. Immunotherapy to treat allergies: Recent advances and future prospects. Juntendo Med J 61:597-600 (2015) [Review]
21. Takai T, Izuhara K. Allergens in modern society: Updated catalogs and future prospects. Allergol Int 64:293-294 (2015) [Editorial]
22. Pichler U, Hauser M, Wolf M, Bernardi ML, Gadermaier G, Weiss R, Ebner C, Yokoi H, Takai T, Didierlaurent A, Rafaiani C, Briza P, Mari A, Behrendt H, Wallner M, Ferreira F. Pectate lyase pollen allergens: Sensitization profiles and cross-reactivity pattern PLoS One. 10:e0120038 (2015)
23. Takai T, Okamoto Y, Okubo K, Nagata M, Sakaguchi M, Fukutomi Y, Saito A, Yasueda H, Masuyama K. Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines–secondary publication. Allergol Int 64:181-186 (2015)
24. Nishikado H, Fujimura T, Taka H, Mineki R, Ogawa H, Okumura K, Takai T, Cysteine protease antigens cleave CD123, the  subunit of murine IL-3 receptor, on basophils and suppress IL-3-mediated basophil expansion. Biochem Biophys Res Commun, 460(2):261-6 (2015)
25. 高井敏朗. 環境アレルゲン解析の進歩. 小児科診療77:1241-1247 (2014)
26. 高井敏朗, 岡本美孝, 大久保公裕, 永田真, 阪口雅弘, 福冨友馬, 齋藤明美, 安枝浩, 増山敬祐. ダニアレルゲンワクチン標準化に関する日本アレルギー学会タスクフォース報告. アレルギー 63:1229-1239 (2014)
27. Zhang C, Ohno T, Kang S, Takai T, Azuma M. Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets. Vaccine. 32:5669-76 (2014)
28. Iida H, Takai T, Hirasawa Y, Kamijo S, Shimura S, Ochi H, Nishioka I, Maruyama N, Ogawa H, Okumura K, Ikeda S. Epicutaneous administration of papain induces IgE and IgG responses in a cysteine protease activity-dependent manner. Allergol Int 63:319-226 (2014)
29. Takai T, Chen X, Xie Y, Vu AT, Le TA, Kinoshita H, Kawasaki J, Kamijo S, Hara M, Ushio H, Baba T, Hiramatsu K, Ikeda S, Ogawa H, Okumura K. TSLP expression induced via Toll-like receptor pathways in human keratinocytes. Methods Enzymol 535:371-387 (2014)
30. Kamijo S, Takeda H, Tokura T, Suzuki M, Inui K, Hara M, Matsuda H, Matsuda A, Oboki K, Ohno T, Saito H, Nakae S, Sudo K, Suto H, Ichikawa S, Ogawa H, Okumura K, Takai T. IL-33-mediated innate response and adaptive immune cells contribute to maximum responses of protease allergen-induced allergic airway inflammation. J Immunol 190:4489-99 (2013)
31. Chen X, Takai T, Xie Y, François Niyonsaba. Okumura K. Ogawa H. Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes. Biochem Biophys Res Commun 433:532-7 (2013)
32. Nakazawa T, Khan AF, Yasueda H, Saito A, Fukutomi Y, Takai T, Zaman K, Yunus M, Takeuchi H, Iwata T, Akiyama K: Immunization of rabbits with nematode Ascaris lumbricoides antigens induces antibodies cross-reactive to house dust mite Dermatophagoides farinae antigens. Biosc Biotechnol Biochem 77:145-150 (2013)
33. Takai T, Mite endopeptidase 1. In: Neil D. Rawlings and Guy S. Salvesen, editors, Handbook of Proteolytic Enzymes. Oxford: Academic Press, 2013, pp.1957-1963.
34. Takai T, Serine endopeptidase allergens from Dermatophagoides species. In: Neil D. Rawlings and Guy S. Salvesen, editors, Handbook of Proteolytic Enzymes. Oxford: Academic Press, 2013, pp.3055-3060.
35. 高井敏朗：アレルゲンはなぜアレルゲンになるのか？：アレルゲンが引き起こすバリア破壊と自然免疫応答.　実験医学 31: 2713-2719 (2013)
36. 高井敏朗：ダニアレルゲンによる経皮感作のメカニズム. アレルギー・免疫 20:854-860 (2013)
37. Xie Y, Takai T, Chen X, Okumura K, Ogawa H: Long TSLP transcript expression and release of TSLP induced by TLR ligands and cytokines in human keratinocytes. 66:233-237 (2012)
38. Saeki M, Nishimura T, Kaminuma O, Suzuki K, Takai T, Mori A, Takada K, Takaiwa F, Hiroi T. Inhibition of allergen-induced airway inflammation by low-dose oral immunotherapy with transgenic rice seeds independently of immunoglobulin E synthesis. Int Arch Allergy Immunol 158:66-69 (2012)
39. Takai T. TSLP expression: cellular sources, triggers, and regulatory mechanisms. Allergol Int 61:3-17 (2012)
40. 高井敏朗：アレルゲンの３つの顔. アレルギー61:930-940 (2012)
41. 高井敏朗：私は思う：アレルゲンとは何か．アレルギーの臨床 32:739-741 (2012)
42. 高井敏朗、池田志斈：ダニアレルギーとアレルゲン週刊日本医事新報 4582号57-59（2012）
43. 高井敏朗：Th2アジュバントとしての環境アレルゲンと黄色ブドウ菌：経皮的Th2感作におけるバリア破壊と自然免疫応答日本皮膚アレルギー・接触皮膚炎学会雑誌 6:8-13 (2012)
44. 高井敏朗：アレルゲンの持つアジュバント作用と抗原感作機構アレルギー・免疫 19:12-19（2012）
45. Vu AT, Chen X, Xie Y, Kamijo S, Ushio H, Kawasaki J, Hara M, Ikeda S, Okumura K, Ogawa H, Takai T. Extracellular double-stranded RNA induces TSLP via an endosomal acidification- and NF-kB-dependent pathway in human keratinocytes. J Invest Dermatol 131:2205-2212 (2011)
46. Kawasaki J, Ushio H, Kinoshita H, Fukai T, Niyonsaba F, Takai T, Ogawa H, Okumura K, Ikeda S: Viral infection induces Thymic stromal lymphopoietin (TSLP) in human keratinocyte. J Dermatol Sci 62:131-134 (2011)
47. Takai T, Ikeda S. Barrier dysfunction caused by environmental proteases in the pathogenesis of allergic diseases. Allergol Int 60:25-35 (2011)
48. Le TA, Takai, T, Vu AT, Kinoshita H, Chen X, Ikeda S, Ogawa H, Okumura K. Flagellin induces the expression of thymic stromal lymphopoietin in human keratinocytes via Toll-like receptor 5. Int Arch Allergy Immunol 155:31-37 (2011)
49. Takai T. Missions of protease allergens in the epithelium. Int Arch Allergy Immunol 154:3-5 (2011)
50. Suzuki K, Kaminuma O, Yang L, Takai T, Mori A, Umezu-Goto M, Ohtomo T, Ohmachi Y, Noda Y, Hirose S, Okumura K, Ogawa H, Takada K, Hirasawa M, Hiroi T, Takaiwa F. Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression. Plant Biotechnol J 9:982-990 (2011)
51. 高井敏朗：アレルゲンはなぜアレルゲンになるのか　アレルギー 60:809-816 (2011)
52. 高井敏朗：表皮ケラチノサイトにおけるTSLP産生誘導　臨床免疫・アレルギー科 55:365-370 (2011)
53. Vu AT, Baba T, Chen X, Le TA, Kinoshita H, Xie Y, Kamijo S, Hiramatsu K, Ikeda S, Ogawa H, Okumura K, Takai T. Staphylococcus aureus membrane and diacylated lipopeptide induce thymic stromal lymphopoietin in keratinocytes via the Toll-like receptor 2-Toll-like receptor 6 pathway. J Allergy Clin Immunol 126:985-993.e3 (2010)
54. Hirasawa Y, Takai T, Nakamura T, Mitsuishi K, Gunawan H, Suto H, Ogawa T, Wang XL, Ikeda S, Okumura K, Ogawa H. Staphylococcus aureus extracellular protease causes epidermal barrier dysfunction. J Invest Dermatol 130:614-617 (2010)
55. Takai T. Cedar allergen harvest from tobacco: Plant biotechnology for recombinant allergens. Int Arch Allergy Immunol 153:431-433 (2010)
56. Le TA, Takai T, Vu AT, Kinoshita H, Ikeda S, Ogawa H, Okumura K, Glucocorticoids inhibit double-stranded RNA-induced thymic stromal lymphopoietin release from keratinocytes in atopic cytokine milieu more effectively than tacrolimus. Int Arch Allergy Immunol 153:27-34 (2010)
57. Vu AT, Takai T, Le TA, Kinoshita H, Kamijo S, Hara M, Wang XL, Ogawa T, Ikeda S, Ogawa H, Okumura K. Proinflammatory response in human keratinocytes stimulated with ligands for TLR2, TLR1, and TLR6. Juntendo Medical Journal 56:50-55 (2010)
58. 高井敏朗：ダニに由来するTh2アジュバント活性　臨床免疫・アレルギー科 54:619-624 (2010)
59. 高井敏朗、上條清嗣：花粉のTh2アジュバント活性　臨床免疫・アレルギー科 54:625-630 (2010)
60. 上條清嗣、奥村康、高井敏朗：樹状細胞の花粉成分によるTh2細胞誘導　臨床免疫・アレルギー科 54: 313-322 (2010)
61. 高井敏朗：アレルゲンに対する自然免疫応答呼吸 29:878-883 (2010)
62. 高井敏朗：知っておかなければならないアレルギー疾患の基礎知識 (2) Th2アジュバントとしてのアレルゲン　アレルギー・免疫 17:687-694 (2010)
63. 上條清嗣、奥村康、高井敏朗：花粉による樹状細胞の活性化　臨床免疫・アレルギー科 53: 312-320 (2010)
64. 奥村康、高井敏朗：環境因子とアレルギー疾患—特にアトピー性皮膚炎を中心にー　日中医学24: 47-52 (2010)
65. Takai T, Kato T, Hatanaka H, Inui K, Nakazawa T, Ichikawa S, Mitsuishi K, Ogawa H, Okumura K. Modulation of allergenicity of major house dust mite allergens Der f 1 and Der p 1 by interaction with an endogenous ligand. J Immunol 183:7958-7965 (2009)
66. Kamijo S, Takai T, Kuhara T, Tokura T, Ushio H, Ota M, Harada N, Ogawa H, Okumura K. Cupressaceae pollen grains modulate dendritic cell response and exhibit IgE-inducing adjuvant activity in vivo. J Immunol 183: 6087-6094 (2009)
67. Kato T, Takai T, Fujimura T, Matsuoka H, Ogawa T, Murayama K, Ishii A, Ikeda S, Okumura K, Ogawa H. Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes Allergy 64:1366-74 (2009)
68. Takai T, Vu AT, Le TA, Kinoshita H, Ushio H, Ikeda S, Ogawa H, Okumura K. Reply. J Allergy Clin Immunol 124:864-5 (2009)
69. Ichikawa S, Takai T, Yashiki T, Takahashi S, Okumura K, Ogawa H, Kohda D, Hatanaka H. Lipopolysaccharide binding of the mite allergen Der f 2. Genes Cells 14:1055-65 (2009).
70. Wang XL, Takai T, Kamijo S, Gunawan H, Ogawa H, Okumura K. NADPH oxidase activity in allergenic pollen grains of different plant species. Biochem Biobhys Res Commun 387:430-434 (2009)
71. Le TA, Takai T, Kinoshita H, Suto H, Ikeda S, Okumura K, Ogawa H. Inhibition of double-stranded RNA-induced TSLP in human keratinocytes by glucocorticoids. Allergy 64:1231-1232 (2009)
72. Suzuki K, Kaminuma O, Yang L, Motoi Y, Takai T, Ichikawa S, Okumura K, Ogawa H, Mori A, Takaiwa F, Hiroi T. Development of transgenic rice expressing mite antigen for a new concept of immunotherapy. Int Arch Allergy Immunol 149 Suppl 1:21-24 (2009)
73. Takai T, Ochiai Y, Ichikawa S, Sato E, Ogawa T, Tokura T, Kuhara T, Kawai H, Hatanaka H, Takakhashi S, Ogawa H, Okumura K. Enzyme-linked immunosorbent assays with high sensitivity for antigen-specific and total murine IgE: Useful tool for study of allergies in mouse models. Allergol Int 58:225-235 (2009).
74. Seto T, Takai T, Ebihara N, Matsuoka H, Wang XL, Ishii A, Ogawa H, Murakami A, Okumura K. SLPI prevents cytokine release in mite protease-exposed conjunctival epithelial cells. Biochem Biobhys Res Commun 379:681-685 (2009)
75. Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Vu AT, Kawasaki J, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol123:179-86 (2009)
76. 高井敏朗：アレルゲン研究の最前線実験医学（増刊号：アレルギー疾患の免疫機構） 27:3340-3347 (2009)
77. 高井敏朗：ダニのアレルゲン活性とプロテアーゼ臨床免疫アレルギー科 52:669-676. 2009
78. 高井敏朗：アレルゲンに関する臨床・基礎の最近のトピックスMinophagen Medical Review 54:71-83 (2009)
79. 高井敏朗：抗原の成り立ち(5)新しい形のアレルゲンワクチン. Q&Aでわかるアレルギー疾患 5(3):338-342 (2009)
80. 高井敏朗：抗原の成り立ち(4)アレルゲンはなぜアレルゲンになるのか？. Q&Aでわかるアレルギー疾患 5(2):218-221 (2009)
81. 高井敏朗：抗原の成り立ち(3)アレルゲン分子のいろいろ. Q&Aでわかるアレルギー疾患 5(1):96-99 (2009)
82. Gunawan H, Takai T, Kamijo S, Wang XL, Ikeda S, Okumura K, Ogawa H: Characterization of proteases, proteins, and eicosanoid-like substances in soluble extracts from allergenic pollen grains Int Arch Allergy Immunol 147:276-288 (2008).
83. Ogawa T, Takai T, Kato T, Kikuchi T, Niyonsaba F, Ikeda S, Okumura K, Ogawa H: Upregulation of release of granulocyte-macrophage colony-stimulating factor from keratinocytes stimulated with cysteine protease activity of recombinant major mite allergens, Der f 1 and Der p 1. Int Arch Allergy Immunol 146:27-35 (2008).
84. Gunawan H, Takai T, Ikeda S, Okumura K, Ogawa H: Protease activity of allergenic pollen of cedar, cypress, juniper, birch, and ragweed. Allergol Int 57:83-91 (2008).
85. 高井敏朗：抗原の成り立ち(2)アレルギー原因物質からアレルゲン分子へ. Q&Aでわかるアレルギー疾患 4(6): 602-605 (2008)
86. 高井敏朗：抗原の成り立ち(1)アレルゲンに対する獲得免疫：IgE抗体とTh2応答. Q&Aでわかるアレルギー疾患 4(5): 526-529 (2008)
87. 高井敏朗：アレルゲンに含有されるプロテアーゼとアレルギー反応の誘導. 臨床免疫アレルギー科50(6):625-631 (2008)
88. 高井敏朗：組換体アレルゲンワクチン. アレルギーの臨床 28(10):865-870 (2008)
89. 高井敏朗：ダニ主要アレルゲンの構造・機能と疾患誘導の関連性の解析およびワクチン設計：リコンビナントアレルゲンの応用．[第１７回アボットジャパン・アレルギー学術奨励賞]アレルギア第36号, 60-64 (2007)
90. Nakamura T, Hirasawa Y, Takai T, Mitsuishi K, Okuda M, Kato T, Okumura K, Ikeda S, Ogawa H.: Reduction of skin barrier function by proteolytic activity of a recombinant house dust mite allergen Der f 1. J Invest Dermatol 126:2719-2723(2006)
91. Kikuchi Y, Takai T, Kuhara T, Ota M, Kato T, Hatanaka H, Ichikawa S, Tokura T, Akiba H, Mitsuishi K, Ikeda S, Okumura K, Ogawa H: Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p 1 to sensitization towards IgE and IgG responses. J Immunol 177:1609-1617 (2006).
92. Kikuchi Y, Takai T, Ota M, Kato T, Takeda K, Mitsuishi K, Ikeda S, Okumura K, Ogawa H: Application of immunoreaction enhancer solutions to an enzyme-linked immunosorbent assay for antigen-specific IgE in mice immunized with recombinant major mite allergens or ovalbumin. Int Arch Allergy Immunol 141:322-330 (2006).
93. Takai T, Mizuuchi E, Kikuchi Y, Nagamune T, Okumura K, Ogawa H: Glycosylation of recombinant proforms of major house dust mite allergens Der p 1 and Der f 1 decelerates the speed of maturation Int Arch Allergy Immunol 139:181-187 (2006).
94. 高井敏朗：プロテアーゼとしてのダニ抗原の意義．先端医療シリーズ３８皮膚疾患の最新医療（斎田俊明、飯塚一編）、先端医療技術研究所、東京、p90-94 (2006)
95. 高井敏朗：ダニアレルゲン研究の現状. 臨床免疫・アレルギー科 46: 619-625 (2006)
96. 高井敏朗：ダニグループ１アレルゲン組換体の作製とその活性．アレルギー・免疫 19: 332-339 (2006)
97. Kato T, Takai T, Mitsuishi K, Okumura K, Ogawa H: Cystatin A inhibits IL-8 production by keratinocytes stimulated with Der p 1 and Der f 1: Biochemical skin-barrier against mite cysteine proteases. J Allergy Clin Immunol, 116:169-76 (2005).
98. Takai T, Kato T, Ota M, Yasueda H, Kuhara T, Okumura K, Ogawa H: Recombinant Der p 1 and Der f 1 with in vitro enzymatic activity to cleave human CD23, CD25, and alpha1-antitrypsin, and in vivo IgE-eliciting activity in mice. Int Arch Allergy Immunol, 137:194-200 (2005).
99. Takai T, Kato T, Yasueda H, Okumura K, Ogawa H. Analysis of the structure and allergenicity of recombinant pro- and mature Der p 1 and Der f 1: Major conformational IgE-epitopes blocked by prodomains. J Allergy Clin Immunol 115:555-563 (2005).
100. Nakazawa T, Takai T, Hatanaka H, Mizuuchi E, Nagamune T, Okumura K, Ogawa H: Multiple-mutation at a potential ligand-binding region decreased allergenicity of a mite allergen Der f 2 without disrupting global structure. FEBS Lett 579:1988-1994 (2005).
101. Takai T, Kato T, Sakata Y, Yasueda H, Izuhara K, Okumura K, Ogawa H: Recombinant Der p 1 and Der f 1 exhibit cysteine protease activity but no serine protease activity. Biochem Biophys Res Commun 328:944-52 (2005).
102. Takai T, Takaoka M, Yasueda H, Okumura K, Ogawa H: Dilution-method to refold bacterially expressed recombinant Der f 2 and Der p 2 to exhibit the secondary structure and histamine-releasing activity of natural allergens. Int Arch Allergy Immunol 137:1-8 (2005).
103. Ichikawa S, Takai T, Inoue T, Yuuki T, Okumura Y, Ogura K, Inagaki F, Hatanaka H: NMR study on the major mite allergen Der f 2: Its refined tertiary structure, epitopes for monoclonal antibodies and characteristics shared by ML protein group members. J Biochem 137:255-263 (2005).
104. 高井敏朗：IgE産生とイエチリダニ由来プロテアーゼ．臨床免疫 44:541-545 (2005)
105. 高井敏朗：ダニ主要アレルゲンの構造と機能．臨床検査 49:739-747 (2005)
106. Sakata Y, Arima K, Takeshita K, Takai T, Aoki S, Ogawa H, Sugihara H, Fujimoto K, Izuhara K. Characterization of novel squamous cell carcinoma antigen-related molecules in mice. Biochem Biophys Res Commun 324:1340-1345 (2004).
107. Sakata Y, Arima K, Takai T, Sakurai W, Masumoto K, Yuyama N, Suminami Y, Kishi F, Yamashita T, Kato T, Ogawa H, Fujimoto K, Matsuo Y, Sugita Y, Izuhara K. The squamous cell carcinoma antigen 2 inhibits the cysteine proteinase activity of a major mite allergen, Der p 1. J Biol Chem. 279:5081-7 (2004).
108. 高井敏朗: アレルゲンの構造と機能：アレルギー発症との関連と、治療への新しいアプローチ. 喘息 17: 15-20 (2004)
109. Roeber D, Achari A, Takai T, Okumura Y, Scott DL. Crystallization and preliminary X-ray analysis of Der f 2, a potent allergen derived from the house dust mite (Dermatophagoides farinae). Acta Cryst D59:1046-1048 (2003).
110. Takai T, Mineki R, Nakazawa T, Takaoka M, Yasueda H, Murayama K, Okumura K, Ogawa H. Maturation of recombinant mite allergen Der p 1 and Der f 1, and its implication in the blockade of proteplytic activity. FEBS Lett 531:265-272 (2002)
111. 高井敏朗: アレルゲンエンジニアリング —アレルゲンの構造・機能とアレルギー・ネットワークの理解を基盤とした次世代の治療戦略—. 臨床免疫 38:298-305 (2002)
112. 高井敏朗: ダニ抗原のエンジニアリング—. アレルギー科 14:285-291
113. 羅智靖、高橋恭子、西山千春、高井敏朗、柳原行義　高親和性IgEレセプター(FceRI)を標的としたマスト細胞活性化制御の展望アレルギー科14,73-82 (2002)
114. Yasuhara T, Takai T, Yuuki T, Okudaira H, Okumura Y. Biologically active recombinant forms of a major house dust mite group 1 allergen Der f 1 with full activities of both cysteine protease and IgE binding. Clin Exp Allergy 31:116-124 (2001).
115. Takahashi K, Takai T, Yasuhara T, Yokota T, Okumura Y. Effects of site-directed mutagenesis in the cysteine residues and the N-glycosylation motif in recombinant Der f 1 on secretion and protease activity. Int Arch Allergy Immunol 124:454-460 (2001).
116. Takai T, Okumura K, Ra C, Yokota T, Okumura Y. Expression of humanized Fab fragments that recognize the IgE-binding domain of human FcεRI α chain. J Biochem 129:5-12 (2001).
117. Takai T, Hatanaka H, Ichikawa S, Yokota T, Inagaki F, Okumura Y. Effects of double-mutation at two distant IgE-binding sites in the three dimensional structure of the major house dust mite allergen Der f 2 on IgE-binding and histamine releasing activity. Biosc Biotechnol Biochem 65:1601-1609 (2001).
118. Takai T, Akagawa-Chihara M, Yokota T, Okumura Y. 2001. Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting. Biosc Biotechnol Biochem 65:694-697 (2001).
119. Yasuhara T, Takai T, Yuuki T, Okudaira H, Okumura Y. 2001. Cloning and expression of cDNA encoding the complete prepro-form of an isoform of Der f 1, the major group 1 allergen from house dust mite Dermatophagoides farinae. Biosc Biotechnol Biochem 65:563-569 (2001).
120. Takai T, Takahashi K, Akagawa-Chihara M, Fukada M, Yuuki T, Shibuya I, Okumura K, Ra C, Yokota T, Okumura Y. Production of humanized antibody against human high-affinity IgE receptor in a serum free culture of CHO cells and purification of the Fab fragments. Biosc Biotechnol Biochem 65:1082-1089 (2001).
121. Takai T, Okumura K, Ra C. Direct expression of the extracellular portion of human FcεRI α chain in Escherichia coli. Biosc Biotechnol Biochem 65:79-85 (2001).
122. Takai T, Ichikawa S, Yokota T, Hatanaka H, Inagaki F, Okumura Y. Unlocking the allergenic structure of the major house dust mite allergen Der f 2 by elimination of key intramolecular interactions. FEBS Letters 482:102-107 (2000)
123. Takai T, Ichikawa S, Hatanaka H, Inagaki F, Okumura Y. Effects of proline mutations in the major house dust mite allergen Der f 2 on IgE-binding and histamine-releasing activity. European Journal of Biochemistry 267:6650-6656 (2000)
124. Takai T, Yuuki T, Ra C. Inhibition of IgE-dependent histamine release from human peripheral blood basophils by humanized Fab fragments that recognize the membrane proximal domain of the human FcεRI α chain. International Archives of Allergy and Immunology 123:308-318 (2000)
125. Takahashi K, Takai T, Yasuhara T, Yuuki T, Ohtake Y, Yokota T, Okumura Y. Production of enzymatically and immunologically active Der f 1 in Escherichia coli. International Archives of Allergy and Immunology 122:108-114 (2000)
126. Takahashi K, Yuuki T, Takai T, Ra C, Yokota T, Okumura Y. Production of humanized Fab fragment against human high affinity IgE receptor in Pichia pastoris. Bioscience, Biotechnology, Biochemistry 64:2138-2144 (2000)
127. Takai T, Yuuki T, Iwamoto-Yasue N, Okumura K, Ra C. 2000. Epitope analysis and primary structures of variable regions of anti-human FcεRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Bioscience, Biotechnology, and Biochemistry 64:1856-1867 (2000)
128. 高井敏朗: 減感作療法への応用を目的とした“アレルゲン・エンジニアリング”—遺伝子工学的に改変したダニ主要アレルゲンDer f 2におけるIgEエピトープの破壊とT細胞エピトープの温存—．アレルギー科10:173-178 (2000)
129. 高井敏朗: アレルゲンエンジニアリング”—ダニ主要アレルゲンDer f 2の改変—．医学のあゆみ192:983-988 (2000)
130. Takai T, Mori A, Yuuki T, Okudaira H, Okumura Y. Non-anaphylactic combination of partially deleted fragments of the major house dust mite allergen Der f 2 for allergen-specific immunotherapy. Molecular Immunology 36:1055-1065 (1999)
131. Yasue M, Yokota T, Fukada M, Takai T, Suko M, Okudaira H, Okumura Y. 1998. Hyposensitization to allergic reaction in rDer f 2-sensitized mice by the intranasal administration of a mutant of rDer f 2, C8/119S. Clinical and Experimental Immunology 113:1-9 (1998)
132. 安江正明、高井敏朗、中村伸、羅智靖．ヒト型抗FcεRI抗体によるアレルギーの制御．アレルギー科6:388-394 (1998)
133. 高井敏朗、羅智靖: 抗IgE, 抗IgE受容体療法．現代医療 30:847-852 (1998)
134. Takai T, Yokota T, Yasue M, Nishiyama C, Yuuki T, Mori A, Okudaira H, Okumura Y. 1997. Engineering of the major house dust mite allergen Der f 2 for allergen-specific immunotherapy. Nature Biotechnology 15:754-758.
135. Takai T, Yuuki T, Okumura Y, Mori A, Okudaira H. 1997. Determination of the N- and C-terminal sequences required to bind human IgE of the major house dust mite allergen Der f 2 and epitope mapping for monoclonal antibodies. Molecular Immunology 34:255-261.
136. Takai T, Yuuki T, Okumura K, Ra C. 1997. An approach to control allergic reaction by blocking the binding of IgE to the high-affinity IgE receptor (FcεRI): Fab fragments of humanized anti-human FcεRIα antibody prevent degranulation of human basophils. Japanese Journal of Inflammation 17:557-562.
137. 羅智靖、高井敏朗．1997. IgE-FcεRI-マスト細胞枢軸を標的にしたアレルギーの新しい治療戦略．モレキュラーメディシン34:1534-1541 (1997)
138. 高井敏朗、羅智靖．ヒト型化抗体．アレルギー科4:478-484 (1997)
139. 高井敏朗、羅智靖．IgE結合阻害薬．内科 76:760-766 (1995)
140. 高井敏朗、羅智靖．高親和性IgE受容体．小児科臨床 57(Suppl.):27-32 (1994)
141. Nishiyama C, Yuuki T, Takai T, Okumura Y, Okudaira H. 1993. Determination of three disulfide bonds in a major house dust mite allergen, Der f II. International Archives of Allergy and Immunology 101:159-166.
142. 奥平博一、須甲松伸、結城敏文、高井敏朗、岩本奈美子、奥村康、安藤徹、猪好孝、山川洋志、岩城正廣．ダニアレルゲンの分子遺伝学的解析．Medical Immunology 23:125-133 (1992)
143. Nakano H, Takai T, Kawakami Y, Nishimura H, Iwakura M 1991. Secretion of a cytoplasmic protein using Escherichia coli α-hemolysin membrane transport system. Kagaku Kogaku Ronbunshu 17:687-693.

研究者

教育活動

ホームページURL

【学校法人順天堂】研究者情報データベース

研究業績詳細

高井 敏朗（タカイ トシロウ）

高井敏朗（タカイトシロウ）