Juntendo University, Tokyo, established in 1838.

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Laboratory of Molecular and Biochemical Research

Introduction

The purpose of our laboratory (Kyodo-ken) is to assist scientific researchers in Juntendo University in their biochemical and molecular biological studies. Kyodo-ken also helps those who want to start their career as a researcher. For these reasons, Kyodo-ken consists of two facilities; training facility and research facility.

Training Facility:

Users can learn the following basic techniques that are required for biochemical and molecular biological studies.
  • RNA preparation
  • Reverse transcription reaction
  • Conventional PCR
  • Real-time PCR
  • Microarray
  • DNA sequencing
  • Cloning
  • Cell culture
  • Biosafety training (How to use P2 level facility)
  • ELISA・Cytotoxicity measurement
  • Protein assay
  • Microelectrode array (MEA)
  • Ca2+ imaging
  • Other basic techniques for gene manipulation (for example, agarose electrophoresis)

Research Facility:

In addition to general experimental room, we have 3 special experimental rooms (P1A level room, cell culture room, and P2 level cell culture room). Users can use laboratory benches, and experimental instruments, equipments and apparatus. Main equipments are as follows.
  • DNA sequencer
  • Real-time PCR system
  • PCR apparatus
  • Fluorescent microscope
  • Microarray system
  • Electroporator
  • Microplate reader
  • Luminometer
  • MEA system
  • Ca2+ imaging system

How to Use the Facility

Qualification of Users: Users must be students, researchers, and technicians who belong to Juntendo University.
Registration; Users must register before use. Registration must be renewed annually.
Reservation; Most of equipments, including analytical instruments and laboratory benches, need reservation.
Instruction; Before use of any instruments, users must learn how to use from staff members.

For details, please feel free to ask staff members.

Staff Members

Chief:
Hiroshi Koide (Room 5S31) e-mail: h-koide@juntendo.ac.jp
Hiroshi Koide
Staffs:
Tomomi Ikeda (Room 5S31)
Takako Ikegami (Room 5S31)
Ryota Hashimoto (Room 5S31)
Mutsuko Hara (Atopy Research Center)

Contact Us

2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan
5th Floor (5S31), Building A.
TEL:03-5802-1110 (Direct line) 
   03-3813-3111 ext. 3611
e-mail: kyodoken@juntendo.ac.jp