Laboratory of Molecular and Biochemical Research
Laboratory
Laboratory Map
Location:Building A, 5th Floor
Training Area
Here, you can learn the following techniques from our staff.- RNA preparation
- Reverse transcription reaction
- Conventional PCR
- Real-time PCR
- Microarray
- DNA sequencing
- Cell culture
- Biosafety training (How to use P2 level facility)
- ELISA
- Cytotoxicity measurement
- Microelectrode array (MEA)
- Ca2+ imaging
- Other basic techniques for gene manipulation
P2 level cell culture room(#5S05)
Here, you can perform experiments using viruses, including adenovirus and retrovirus.
Instruments available
- Safety Cabinet(2) (MHE-130AJ, Panasonic/NSE-1200ⅡB2, Dalton)
- CO2 Incubator (Model F370, Thermo / 9200E, 9300E, Waken)
- Low Speed Refrigerated Centrifuge (Himac CF7D2, Hitachi)
- High Speed Refrigerated Microcentrifuge (Himac CT13R, Hitachi)
- Fluorescent microscope (BZ-9000, Keyence)
- Microscope (TS-100, Nikon)
- Autoclave (SX-500, Tomy)
- Deep Freezer (-80℃) (MDE-U32V, Panasonic)
Cell culture room (#5S11)
Here, you can culture primary cells and established cell lines.
Instruments available
- Clean benches (3) (Panasonic, JUJIFIELD, Showa kagaku)
- Safety Cabinet(2) (1345, Thermo /MHE-S901A2-PJ, Panasonic)
- CO2 Incubators (11) ( Model F370, Thermo / 9100Ex,9200Ex,Waken)
- Low Speed Refrigerated Centrifuge (Himac CF7D2, Hitachi)
- Fluorescent microscope (BZ-X800, Keyence)
- Microscopes (2) (Axiovert40, Primovert, Zeiss)
- Electroporator (3) (Nucleofactor II, Lonza / Neon, Thermo / NEPA Porator System 2, NEPAGENE)
- Microelectrode array system (MEA 2100-Systems, Bio Research Center)
General BSL2 level experiment room(#5S)
Here, you can perform biochemical and molecular biological experiments.
Instruments available
- Safety Cabinet (AC2-3N7,ESCO)
- Microplate reader (SpectraMax 340PC384, Molecular Devices)
- Luminometer (2) (Lumat LB9507, Berthold for single test tube / SpectraMaxL, Molecular Device for 96-well plate)
- Thermal cycler (PCR apparatus) (2) (T-100, Bio Rad)
- Fluorescent microscope (2) (BZ-X700, Keyence)
- Trans Illuminator (Printgraph 2M, ATTO)
- UV-Vis Spectrophotometer (NanoDrop, Thermo)
- Automated electrophoresis system (Experion)
P1A level room(#5S39)
English Here, you can sacrifice animals, such as mice and rats.
Equipment room (#5S33)
Here, you can use the following analysis instruments.- Real-time PCR system (4) (7500 Fast Real time PCR systems, QuantStudio 3, Thermo)
- DNA sequencer(2) (3500/3730 Genetic Analyzer, Thermo)
- MicroArray system (GeneChip system, Thermo)
Main Instruments Click here to view all instruments
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Real-time PCR system (Thermo Fisher Scientific) QuantStudio 3 Real-time PCR System, 7500 Fast Real-Time PCR System |
DNA sequencer (Thermo Fisher Scientific) 3500 Genetic Analyzer, 3730 Genetic Analyzer |
Microarray (Thermo Fisher Scientific) GeneChip system |
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Automated electrophoresis system (Bio Rad) Experion |
Microplate Reader (Molecular Devices) SpectraMax 340 PC384 |
Luminometer (Molecular Devices) SpectraMax L |
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Microelectrode array system (Bio Research Center) MEA 2100-Systems |
Ca2+ imaging system
(Hamamatsu Photonics) AquaCosmos imaging system |
Fluorescent microscope (Keyence) BZ-X800, BZ-X700, |
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Electroporator | ||
(NEPAGENE) NEPA Porator System2 |
(Thermo Fisher Scientific) Neon Transfection System |
(Lonza) Nucleofector Ⅱ Device |